Quantitative detection instrument and detection method of immunochromatography test strip

Abstract

The invention relates to an immunochromatography quantitative detection instrument and a detection method of colloidal gold, latex and the like for quantitative detection of an immunochromatography test strip. A to-be-detected sample is dripped onto a transverse immunochromatography test strip which is marked in advance to coat a corresponding antigen-antibody, then the test strip is inserted into a detection tank of the immunochromatography quantitative detection instrument which is used for confirming the color chromaticity and background chromaticity of C and T line regional positions in a nitrocellulose membrane of the test strip by virtue of multi-wavelength scanning, identification computational analysis is performed on optical density values of a quality control band and a detection band according to a built-in algorithm of the instrument, then the concentration of positive markers contained in the to-be-detected sample is obtained, a report is printed, and a result value is stored. By using the immunochromatography quantitative detection instrument and the detection method, the influence of the difference among different immunochromatography test strips on detection results can be avoided, and precise and stable quantitative detection is really realized. In addition, the immunochromatography quantitative detection instrument and the detection method have the advantages of good open generality and are suitable for determining the detection results of immune test paper with different specifications and different detection types.

Description

technical field [0001] The invention belongs to the technical fields of monoclonal antibody technology, antibody labeling technology, lateral immunochromatography technology and photoelectric sensor technology, and in particular relates to a lateral immunochromatographic detection device capable of quantitative detection such as colloidal gold, latex and other particle labeling technologies And the detection method of the detection device. Background technique [0002] Immunochromatography (immunochromatography) is a rapid diagnostic technique that has risen abroad in recent years. Its principle is to fix specific antibodies on a certain zone of the nitrocellulose membrane first, and when one end of the dry nitrocellulose is immersed in the sample (urine or serum), the sample will move forward along the membrane due to capillary action, and when it moves to the area where the antibody is immobilized, the corresponding antigen in the sample will specifically bind to the antib...

AI Technical Summary

Problems solved by technology

[0004] However, most of the horizontal immunochromatographic devices such as colloidal gold and latex that can be used for automatic quantification on the market so far cannot avoid the influence of the differences between test strips such as colloidal gold and latex on the test results. , can not really achieve more accurate and stable quantitative detection, or has a relatively narrow closedness, does not have good openness and versatility; at the same time, due to the fluctuations in the production and storage process of the existing test strips, the market situation is further aggravated. The difference in the test results of the existing quantitative instruments in the world

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