High-efficiency rapid propagation method of good Rhus chinensis strains

Abstract

The invention discloses a high-efficiency rapid propagation method of good Rhus chinensis strains. The high-efficiency rapid propagation method comprises the following steps: performing root-pressing propagation on the good field perennial Rhus chinensis earth surface root and culturing for 25-40 days to grow a complete plant; carrying out surface sterilization on leaves of the seedling of the complete plant, taking leaf vein and inoculating on an induction medium, performing illumination culture for 28-35 days to enable the two ends of the leaf vein to grow adventitious buds; inoculating the adventitious buds on a multiple-shoot proliferation and extension medium for bud proliferation and extension, transferring to a rooting medium for rooting after buds grow for 2-3cm, thereby obtaining the strong Rhus chinensis test-tube plantlet. The high-efficiency rapid propagation method has the advantages that root-pressing propagation is adopted to strength the seedling, the problem of pollution and browning severity caused by a situation that field materials are directly drawn as the explants is solved, and the method is convenient for realizing regeneration and obtaining tissue culture seedlings.

Description

technical field [0001] The invention relates to a method for high-efficiency and rapid propagation, especially a method for high-efficiency and rapid propagation of excellent strains of A. chinensis. Background technique [0002] Saltwood is a small deciduous tree belonging to the family Anacardiaceae. It has strong adaptability and wide distribution. Its seeds contain a large amount of unsaturated fatty acids, and its oil has high edible value. It is also a potential tree species for bio-energy raw material oil. Oil, ornamental and raw materials for industrial dyes, etc. Saltwood has a high medicinal value, and gallnuts formed by aphid galls parasitic on its leaves are important traditional Chinese medicinal materials and industrial raw materials, and are important forest special products in my country. With the continuous increase of the demand for the related products of Saltwort, it is of great significance to cultivate high-yield and high-quality Saltwort varieties and...

AI Technical Summary

Problems solved by technology

[0003] The commonly used seedling raising methods are seedling raising and cutting root seedling raising, but the seedling raising process is cumbersome, time-consuming and labor-intensive, and the seedling raising time is easily restricted by seasons

At the same time, for the propagation of excellent strains, due to the high heterozygosity of seeds and the large variation of seedlings in the mode of seedling breeding, the characteristics of excellent strains are often not maintained; while root cutting propagation requires a large number of roots, and the materials are limited. Therefore, this method of reproduction cannot be scaled up

With the development of tissue culture technology, researchers try to use different tissues of excellent strains of Salicola chinensis in the field as explants, and use tissue culture technology to quickly propagate the excellent strains, but because the tissues directly taken from the field contain relatively High secondary metabolites, resulting in serious browning and pollution during tissue culture, resulting in difficult regeneration

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