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Method for preparation of deer blood heme by ultrasound and enzymolysis technologies

A technology of ultrasonic wave and heme, applied in the field of food biochemistry, can solve problems such as low yield

Inactive Publication Date: 2014-10-15
HEILONGJIANG PROV WILD ANIMAL INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzymatic extraction of heme is the most environmentally friendly extraction method because it does not use organic solvents, but the yield is low

Method used

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  • Method for preparation of deer blood heme by ultrasound and enzymolysis technologies
  • Method for preparation of deer blood heme by ultrasound and enzymolysis technologies
  • Method for preparation of deer blood heme by ultrasound and enzymolysis technologies

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0021] Specific embodiment 1: This embodiment prepares deer blood heme according to the following method

[0022] (1) Add 1% anticoagulant trisodium citrate solution to fresh deer blood, stir evenly to obtain fresh anticoagulated deer blood; take 100mL of anticoagulated blood in a centrifuge tube, centrifuge at 4500r·min-1 for 25min After decanting the supernatant (serum) to collect the red blood cells, wash with saline three times to obtain clean red blood cells. Add deionized water twice the volume of the red blood cells, stir with an electric mixer for 30 minutes, the red blood cells will absorb water and swell, the hemoglobin will be separated from the red blood cells, the fibers will be filtered out, and the filtrate will be used for later use.

[0023] (2) The spare V red blood cells and V water are configured at a concentration of 1:1 to 1:9, and crushed in an ultrasonic device with a power of 100 to 300w, and the crushing time is 0 to 30min;

[0024] (3) Add alkaline proteas...

specific Embodiment approach 2

[0026] Specific embodiment two: This embodiment is different from the specific embodiment one in that this embodiment prepares deer hemoglobin according to the following method:

[0027] (1) Add 1% anticoagulant trisodium citrate solution to fresh deer blood, stir evenly to obtain fresh anticoagulated deer blood; take 100mL of anticoagulated blood in a centrifuge tube, centrifuge at 4500r·min-1 for 25min After decanting the supernatant (serum) to collect the red blood cells, wash with saline three times to obtain clean red blood cells. ·Add deionized water twice the volume of red blood cells, stir with an electric mixer for 30 minutes, the red blood cells will absorb water and swell and crack, the hemoglobin will be separated from the red blood cells, the fibers will be filtered out, and the filtrate will be used for later use.

[0028] (2) The spare V red blood cells and V water are configured according to the concentration of 1:5, and crushed in a 200w power ultrasonic equipment,...

specific Embodiment approach 3

[0031] Specific embodiment three: this embodiment is different from specific embodiment one in that this embodiment prepares deer hemoglobin according to the following method:

[0032] (1) Add 1% anticoagulant trisodium citrate solution to fresh deer blood, stir evenly to obtain fresh anticoagulated deer blood; take 100mL of anticoagulated blood in a centrifuge tube, centrifuge at 4500r·min-1 for 25min After decanting the supernatant (serum) to collect the red blood cells, wash with saline three times to obtain clean red blood cells. Add deionized water twice the volume of the red blood cells, stir with an electric mixer for 30 minutes, the red blood cells will absorb water and swell, the hemoglobin will be separated from the red blood cells, the fibers will be filtered out, and the filtrate will be used for later use.

[0033] (2) The spare V hemoglobin and V water are configured at a concentration of 1:5, and crushed in an ultrasonic device with a power of 185w for 9 minutes;

[0...

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Abstract

Belonging to the field of food biochemistry, the invention relates to a method for preparation of deer blood heme by ultrasound and enzymolysis technologies. The preparation method includes the steps of: (1) adding a trisodium citrate solution into fresh deer blood, stirring the substances evenly to obtain fresh anticoagulant deer blood, taking 100mL of the anticoagulant blood into a centrifugal tube, conducting centrifugation for 25min, pouring out the supernatant and collecting red blood cells, and performing washing by saline 3 times to obtain clean red blood cells, adding deionized water, conducting stirring by an electric mixer, letting the red blood cells absorb water and burst so as to separate hemoglobin from the red blood cells, filtering out fiber, and leaving the filtrate for standby use; (2) adding certain amount of water into the red blood cells in (1), and conducting crushing in ultrasonic equipment for some time; (3) adding alkaline protease of certain substrate concentration into the solution in (2), adjusting the temperature and pH, and carrying out enzymolysis; and (4) performing separation and freeze drying. Experiments prove that preparation of deer blood heme by ultrasound and enzymolysis technologies can reach an extraction rate of 72%, and the purity of a crude product can reach 31.2%.

Description

Technical field [0001] The invention belongs to the field of food biochemistry, and specifically relates to a method for preparing deer heme by using ultrasonic waves and enzymatic hydrolysis. Background technique [0002] Heme is an excellent iron enhancer and anti-anemia drug. It has been confirmed by pharmacological and clinical researches that it can be directly absorbed by the human body as a blood tonic. It has a significant effect of increasing white blood cells and platelets, with significant curative effect and few side effects; Heme is also a raw material for preparing cancer drugs. Extracting heme from red blood cells of deer can not only prevent and improve iron-deficiency anemia, but also work together or synergistically with the rich active nutrients in deer blood to have great health benefits. Due to the important value of heme, in recent years, researchers have attracted widespread attention and explored many new extraction methods. Enzymatic extraction of heme ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D487/22
CPCC07D487/22
Inventor 王帅刘维斌
Owner HEILONGJIANG PROV WILD ANIMAL INST
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