A fault diagnosis method for penicillin fermentation process based on nuclear partial least squares reconstruction

Abstract

The invention provides a penicillin fermentation process fault diagnosis method based on kernel partial least squares reconstruction, comprising: collecting offline historical normal data of penicillin fermentation process; Standardize and standardize variable offline historical normal data sets; use the improved nuclear partial least squares method to establish a fault monitoring model for the fermentation process of penicillin; monitor the faults of the penicillin fermentation process online; Correlation direction model for fermentation process faults; fault diagnosis for penicillin fermentation process. The invention divides the input space into: the pivot space directly related to the output, the pivot space irrelevant to the output and the residual space irrelevant to the output. Compared with the traditional method, it not only monitors the input variables related to the output, but also accurately monitors the variables related to the input.

Description

technical field [0001] The invention belongs to the technical field of fault monitoring and diagnosis, and in particular relates to a method for fault diagnosis of penicillin fermentation process based on nuclear partial least squares reconstruction. Background technique [0002] Penicillin is a kind of antibiotic and a secondary metabolite. The schematic diagram of its fermentation process is shown in figure 1 As shown, the entire production cycle includes 4 physiological periods: the reaction lag period, the rapid growth period of the bacteria, the penicillin synthesis period and the death (autolysis) period of the bacteria; 2 physical sub-periods: the cell culture period (also known as the batch operation stage, corresponding to the first two physiological periods, lasting about 45h) and the penicillin fed-feeding fermentation stage (also known as the intermittent feeding operation stage, corresponding to the last two physiological periods, lasting about 355h). During th...

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Problems solved by technology

When controlling foam, it is also necessary to add acid and alkali to adjust the pH value of fermentation. Too much or too little acid and alkali will affect the pH value of fermentation, resulting in the failure of penicillin fermentation

In addition, in the cell culture stage, the concentration of bacteria is low, and the medium is rich in nutrients, so it is easy to form bacterial contamination.

In the early stage of penicillin fermentation, miscellaneous bacteria are easy to reproduce after infection, and compete with the production bacteria for nutrients and oxygen, which seriously interferes with the growth and reproduction of production bacteria and the production of products, disturbs the production order, and disrupts the production plan; the contamination in the middle stage of fermentation will seriously interfere with production The metabolism of bacteria affects the production of products. At this time, the method of "inverting" is generally adopted, which will cause a lot of waste of raw materials and increase of operating costs.

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