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A composition for detecting lung cancer hotspot mutation gene and its application method

A mutated gene and composition technology, which is applied in the field of compositions for detecting hot spot mutations in lung cancer driver genes, can solve the problems of poor detection specificity, inability to meet the needs of lung cancer detection, and low sensitivity.

Active Publication Date: 2016-08-17
JIANGSU MICRODIAG BIOMEDICINE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] However, the sensitivity of these technologies is not high, the specificity of detection is poor, and the misdiagnosis rate is high, which cannot meet the needs of lung cancer detection.

Method used

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  • A composition for detecting lung cancer hotspot mutation gene and its application method
  • A composition for detecting lung cancer hotspot mutation gene and its application method
  • A composition for detecting lung cancer hotspot mutation gene and its application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Example 1. Screening of hot spot gene mutations in lung cancer patients

[0075] 1. Experimental materials

[0076] 1. Collect 30 tissue samples and matching plasma samples from patients with lung cancer diagnosed by pathology. After the plasma sample is collected, it is immediately stored in a -80 degree refrigerator.

[0077] 2. The purity of all primers should be up to electrophoresis level (PAGE) or HPLC level, free of impurities. Provide the quality inspection certificate of the synthesized product issued by the synthesis organization, such as the PAGE electrophoresis result or HPLC analysis pattern, which proves that there should be an obvious single-peak PAGE or HPLC purification pattern after PAGE or HPLC purification, and the concentration is 10ng / μl for use.

[0078] 3. Purchase all reagents from regular manufacturers: DNA polymerase (Roche), 10×PCR Buffer (Roche), MgCl 2 (Roche), dNTP (TaKaRa), purified water.

[0079] 2. Main instruments: Roche Light CyclerTM 480, n...

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Abstract

The invention discloses a composition for detecting a hot-spot mutation gene of a lung cancer. The composition comprises primer sequences in SEQ NO:1 to SEQ NO:53, and block sequences in SEQ NO:54 to SEQ NO:63. The detection method for lung cancer-related mutation has the advantages of strong specificity, high sensitivity, small pollution, simplicity and rapidity of operation, high safety and the like, the detection result has good accuracy and repeatability, and the detection method is especially suitable for detection of hot-spot mutation of a lung cancer driver gene from body fluid such as plasma, is capable of noninvasively carrying out diagnosis, recurrence monitoring and therapeutic effect evaluation on a patient with the lung cancer in real time, and has an important value.

Description

Technical field [0001] The present invention relates to the field of gene detection, in particular to a composition for detecting hot spot mutations in lung cancer driving genes. Background technique [0002] Lung cancer is a kind of malignant tumor with high incidence and high mortality. In the past 10 years, the incidence and mortality of lung cancer in my country have increased rapidly. The location of lung cancer is different. According to the differentiation and morphological characteristics of each type of lung cancer, lung cancer is currently divided into two categories, namely small cell lung cancer and non-small cell lung cancer, the latter including squamous cell carcinoma, adenocarcinoma and large cell carcinoma . [0003] Studies have found that the occurrence of lung cancer is closely related to the mutations of EGFR, KRAS, PIK3CA hotspot genes. [0004] EGFR gene is expressed on the surface of normal epithelial cells, and it is often overexpressed in some tumor cells...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6848C12Q1/6858C12Q2531/113C12Q2527/107C12Q2549/126
Inventor 王弢李宗飞张利清张丽乐飚徐维琛
Owner JIANGSU MICRODIAG BIOMEDICINE TECH CO LTD
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