Multicolor high-risk HPV (human papilloma virus) fluorescence detection kit

A technology of fluorescence detection and kit, which is applied in the field of multi-color high-risk HPV fluorescence detection kits, can solve the problems of low amplification sensitivity, inability to specifically classify high-risk HPV types, and difficult to meet, and achieve high detection throughput and reduce screening. Check the effect of cost

Active Publication Date: 2014-12-10
GUANGZHOU HEAS BIOTECH CO LTD
View PDF4 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The disadvantages of the existing techniques for genotyping detection of high-risk human papillomaviruses by using fluorescent PCR method are mainly low amplification sensitivity and long time-consuming
In the early stage, it is generally necessary to go through te

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Multicolor high-risk HPV (human papilloma virus) fluorescence detection kit
  • Multicolor high-risk HPV (human papilloma virus) fluorescence detection kit
  • Multicolor high-risk HPV (human papilloma virus) fluorescence detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0057] Below in conjunction with embodiment, further illustrate the present invention.

[0058] Three-color high-risk HPV fluorescent detection kit, including DNA extraction solution, hot start Taq enzyme system, universal primers, fluorescent probes, negative control and positive control, fluorescent probes are HPV-specific MGB probes, HPV-specific MGB probes The sequence is as follows:

[0059] Probe 1 TTGGCATAATCAATTATTT (SEQ ID NO: 1)

[0060] Probe 2 TGGGGCAATCAGTTRTTTGT (SEQ ID NO: 2)

[0061] Probe 3 CTGATTGTTCCARCARATG (SEQ ID NO: 3)

[0062] Probe 4 AATCAGTTGTTTGTTACTGT (SEQ ID NO: 4)

[0063] Probe 5TTTRTTACCTGTGTTGATAC (SEQ ID NO: 5)

[0064] Probe 6 TAACTGTTGTGGATACCAC (SEQ ID NO: 6)

[0065] Probe 7 TATATGTTGGCACAATCAA (SEQ ID NO: 7)

[0066] Probe 8 TGGTTACCCCAACAAATG (SEQ ID NO: 8)

[0067] Probe 9 TAATTGATTATGCCAGCAAACA (SEQ ID NO: 9)

[0068] Probe 10 TTACCTGTGTTGATACYAC (SEQ ID NO: 10)

[0069] Probe 11 CTGATTGCCCCAACAAAT (SEQ ID NO: 11)

[0070] Prob...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a multicolor high-risk HPV (human papilloma virus) fluorescence detection kit which comprises a warm-boot Taq enzyme system, universal primers, fluorescent probes, a negative control and a positive control, wherein the fluorescent probes are HPV-specific MGB probes. The kit is suitable for qualitatively detecting 18 high-risk HPV genotypes capable of causing cervical carcinoma in cervix exfoliated cell and genitourinary tract secretion samples. The kit uses the multiplex fluorescence PCR (polymerase chain reaction) technique to implement detection of 18 HPV genotypes in one tube amplification system. The kit can simultaneously detect 15 common high-risk HPV genotypes (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 and 82) and can also detect 3 rare high-risk HPV genotypes (26, 53 and 73). The detection kit has the advantage of high detection flux and greatly lowers the detection cost.

Description

technical field [0001] The invention relates to a HPV fluorescence detection kit, in particular to a multi-color high-risk HPV fluorescence detection kit. Background technique [0002] Cervical cancer is the most common gynecological malignancy. The high-incidence age of carcinoma in situ is 30-35 years old, and that of invasive carcinoma is 45-55 years old. In recent years, the onset tends to be younger. Early detection and treatment of cervical cancer is the key to effective treatment of cervical cancer. [0003] Existing studies have shown that the main cause of cervical cancer is persistent or recurrent infection of high-risk human papillomavirus (HPV). More than 90% of cervical cancers are associated with high-risk HPV infection, and persistent high-risk HPV infection is the main risk factor for cervical cancer. Therefore, HPV DNA testing has become an important part of cervical cancer screening and health management in many countries around the world. There are man...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68
CPCC12Q1/686C12Q1/70C12Q1/708C12Q2531/113C12Q2537/143C12Q2563/107
Inventor 陈华云肖湘文丁渭刘淑园陈嘉昌方凤银赵丽
Owner GUANGZHOU HEAS BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap