A method for improving the preservation effect of lily embryogenic callus
A technology for embryogenic callus and lily, which is applied in the field of improving the preservation effect of lily embryogenic callus, can solve problems such as the establishment of an ultra-low temperature storage system for lily embryogenic callus, achieve a significant optimization of preservation effect, increase viscosity and Thermal conductivity, damage reduction effect
Active Publication Date: 2016-03-09
SHANGHAI JIAO TONG UNIV
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Problems solved by technology
However, there are few studies on the cryopreservation of lily embryogenic callus as material, and the cryopreservation system of lily embryogenic callus has not been established yet.
Method used
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Embodiment
[0053] 1) The lily embryogenic callus subcultured for 20 days was pre-cultured at 4° C. for 2 days on MS solid medium containing 0.7 mol / L sucrose;
[0054] 2) Transfer to the loading solution for soaking at room temperature for 60 minutes;
[0055] 3) Transfer to the vitrification solution and dehydrate at 0°C for 60 minutes;
[0056] 4) Finally, store in liquid nitrogen at ultra-low temperature.
[0057] After step 3), without removing the vitrification solution, directly place the lily embryogenic callus soaked in the vitrification solution in liquid nitrogen for cryopreservation.
[0058] According to the above steps, the embryogenic calli of lily were divided into experimental group and control group.
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The invention discloses a method for improving a preservation effect of lily embryonic callus. According to the invention, the lily embryonic callus is processed by adopting a vitrification solution which contains a carbon nanometer material so as to improve the preservation effect of the lily embryonic callus. The method specifically comprises the steps of pre-culture, contained liquid treatment, vitrification solution treatment and liquid nitrogen preservation, wherein the vitrification solution contains 0.1-0.5 g / L carbon nano-tubes. The method disclosed by the invention optimizes the preservation effect of the lily embryonic callus remarkably; as the carbon nano-tubes are added to serve as an allogenic material, the vitrification ultra-low temperature preservation of plants is facilitated.
Description
technical field [0001] The invention relates to the field of preservation of plants or parts thereof, in particular to a method for improving the preservation effect of lily embryogenic callus. Background technique [0002] Cryopreservation is a modern in vitro preservation technology for germplasm resources developed in the 1970s. Usually stored in liquid nitrogen, the substance metabolism and growth activities in the preserved material cells are almost completely stopped, and they are in a relatively stable biological state, achieving the purpose of long-term preservation of germplasm. Ultra-low temperature preservation is currently the only method that does not require continuous subculture. Medium and long-term preservation method. Vitrification cryopreservation is to place cells or tissues in a vitrification solution composed of a certain proportion of permeable and non-permeable protective agents, so that the material and its vitrification solution solidify into an am...
Claims
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IPC IPC(8): C12N5/04
Inventor 申晓辉陈冠群任丽张荻
Owner SHANGHAI JIAO TONG UNIV
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