Wild abortive type sterility gene PCR (polymerase chain reaction) detecting primer and application thereof

Abstract

The invention discloses a wild abortive type sterility gene PCR (polymerase chain reaction) detecting primer WA7 and a combined primer WA7 / RED4. The combined primer WA7 / RED4 comprises a WA7 positive primer 5'-TTTGCCTTATTTTGACCTGTTTGA-3', a WA7 negative primer 5'-TACTTTCAGGAGGGGAGGCA-3', a RED4 positive primer 5'-TTACAGGGGAGCAGAAACA-3' and a RED4 negative primer 5'-TCTTCTCCTCTCTTTCAGCAC-3'. The combined primer WA7 / RED4 can be adopted to effectively complete identification on hybrid rice, weedy rice and conventional rice by once PCR, and can be used for simply, quickly, accurately and easily realizing batch detection.

Description

technical field [0001] The invention relates to a PCR detection primer for wild abortive sterility gene and combined primers for one-time detection of hybrid rice, weedy rice and conventional rice. Background technique [0002] Asian cultivated rice ( Oryza sativa ) is one of the important food crops in my country. In terms of planting area, conventional rice ( Oryza sativa ), that is, rice varieties that can be kept and the offspring are not separated, accounting for about half; three-line hybrid rice ( Oryza sativa ) Planting also accounted for about half. Among the three-line hybrid rice, wild-type three-line hybrid rice is currently the most important planting type. [0003] In recent years, another Oryza plant—weedy rice ( Oryza sativa ), which is often associated with cultivated rice and is widely distributed, almost existing in all rice production areas in the world. In some rice-growing countries in the temperate zone of the world, the damage has be...

AI Technical Summary

Problems solved by technology

Therefore, when we try to screen hybrid rice, weedy rice, and conventional rice with these primers, we cannot use them at all.

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