Method for breaking paeonia suffruticosa seed dormancy

A peony seed and pathway technology, applied in seed and rhizome treatment, application, agriculture, etc., can solve the problems of high production cost, restricting the booming peony economy, and low production efficiency, and achieve the effect of shortening the time span

Inactive Publication Date: 2015-03-25
王阿慧
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Under normal circumstances, it takes two years for peony seeds to break dormancy due to overwintering and low temperature, and it takes about 10 years to blo

Method used

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Embodiment Construction

[0017] The present invention will be further elaborated below in conjunction with specific embodiment:

[0018] 1. Plant material: Fengdan seeds, from 2010 to 2012, collected in the peony breeding nursery of Shanghai Botanical Garden Research Center every September, removed pods after harvest, dried in a cool place, packed in plastic bags, 4°C or -20°C Store in the refrigerator for later use.

[0019] 2. Material treatment: select mature and plump peony seeds, wash them with tap water for 2-4 hours, and blot them dry with filter paper; then soak them in 70% alcohol for 60 seconds, rinse them with sterile water or distilled water for 3 times, blot them dry with filter paper, and store them at 4°C or -20°C. ℃ refrigerator for future use.

[0020] Mother liquor preparation: 1000 mg / L gibberellin (GA 3 ) solution, dissolved with analytical pure alcohol, ready to use; 1 mg / L BA, dissolved with 1 mol / L HCl, stored in refrigerator at 4°C; 5 mg / L Ca(NO 3 ) 2 , directly soluble in ...

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Abstract

The invention provides a method for breaking paeonia suffruticosa seed dormancy. The invention establishes a complete set of technical system for breaking paeonia suffruticosa seed dormancy. The system is divided into two ways: seedling and in vitro embryos. The seedling way operation steps include: conducting soaking with 1000mg/L GA3 for 24h; performing storage in wet sand with 100mg/L GA3+1mg/L BA+3mg/L Ca(NO3)2 at 4DEG C; carrying out sowing in a matrix of peat, vegetable garden soil and perlite (3:6:1); and conducting foliage spraying with 10mg/L GA3+1mg/L BA+3mg/L Ca(NO3)2. The in vitro embryo way operation steps include: performing soaking with 1000mg/L GA3 for 24h; removing hulls and conducting soaking with 100mg/L GA3+1mg/L BA+3mg/L Ca(NO3)2 for 24h; and adding the seeds into a WPM medium containing 5mg/L GA3+1mg/L BA+3mg/L Ca(NO3)2 for 4 weeks. The method provided by the invention can simply, rapidly and efficiently break paeonia suffruticosa seed dormancy, greatly shortens the time span of paeonia suffruticosa peony seedling formation and flowering, and provides a brand new technology platform for follow-up development and utilization of paeonia suffruticosa, Chinese herbaceous peony and other species with dormancy characteristics.

Description

technical field [0001] The invention relates to the field of biology, in particular to a method for releasing peony seeds from dormancy. Background technique [0002] Peony (Paeonia suffruticosa), also known as rich flower, hundred taels of gold, wood peony, rat aunt, deer leek, Luoyang flower, is a woody plant of Paeoniaceae Paeoniae, a deciduous shrub, known as "national beauty and heavenly fragrance", "the best flower among flowers". The reputation of "King" is the first choice for my country's national flower. Peony not only has high ornamental value, but also has good medicinal value. As early as the Han Dynasty, there was a record of peonies being domesticated and used as medicine in the ancient book of medicinal plants "Shen Nong's Materia Medica". With the increase of the market value of peony in landscaping and medicinal material storage, the economic market of peony has been born. The whole market has formed a complete system from seedlings, potted flowers, cut fl...

Claims

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Application Information

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IPC IPC(8): A01C1/00
Inventor 王阿慧
Owner 王阿慧
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