Method for keeping toxicity of beauveria bassiana to host insects

Abstract

The invention discloses a method for keeping the toxicity of beauveria bassiana to host insects. The method comprises the steps of adding ground host insects into a beauveria bassiana PDA culture medium, and inoculating beauveria bassiana spores after sterilizing; then, culturing in an illumination incubator at a proper temperature; after mycelia grow to generate spores, scraping the spores by using a scraper in an aseptic operating platform; and carrying out liquid fermentation culture or preparing a suspension with a certain concentration to be directly used for preventing and controlling pests. Compared with the prior art, the method has the advantages that the toxicity of the beauveria bassiana to the host insects can be kept for a long term, and the beauveria bassiana can be domesticated according to different host insects to prepare a special insecticide for certain pests, so that more effective preventing and controlling effects can be achieved. In addition, strains domesticated according to different host insects can be mixed to apply according to the kinds of the prevented and controlled pests, so that the effect is better.

Description

technical field [0001] The invention relates to a method for maintaining the toxicity of Beauveria bassiana to host insects. Background technique [0002] Beauveria bassiana (Verticillium lecanii) is an entomopathogenic fungus belonging to the subclass Rhizocycetes Verticillium creeping group, and its host range includes Coleoptera, Lepidoptera, Hemiptera, Thysanoptera order, Orthoptera, Hymenoptera, Diptera, Collembola, gall mites, nematodes, and even parasitic plant pathogens. It has good genetic stability and is harmless to humans, livestock, poultry and plants. It is a kind of Biocontrol fungi with broad application prospects, but in the application process, after several generations of continuous inoculation and culture on artificial medium, their toxicity to pests will be significantly reduced or even lost, which seriously restricts the further development of Beauveria bassiana resources. develop and use. Therefore, it is urgent to establish a method that is suitable...

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Problems solved by technology

[0002] Beauveria bassiana (Verticillium lecanii) is an entomopathogenic fungus belonging to the subclass Rhizocycetes Verticillium creeping group, and its host range includes Coleoptera, Lepidoptera, Hemiptera, Thysanoptera order, Orthoptera, Hymenoptera, Diptera, Collembola, gall mites, nematodes, and even parasitic plant pathogens. It has good genetic stability and is harmless to humans, livestock, poultry and plants. It is a kind of Biocontrol fungi with broad application prospects, but in the application process, after several generations of continuous inoculation and culture on artificial medium, their toxicity to pests will be significantly reduced or even lost, which seriously restricts the further development of Beauveria bassiana resources. develop and use

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