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A fluorochrome probe

A fluorescent dye and probe technology, applied in the field of fluorescent dye probes, can solve the problem of no fluorescent dye labeling method, and achieve excellent super-resolution microscopy imaging capability, high resolution, excellent activation-free and anti-bleaching effects.

Active Publication Date: 2015-04-01
SINGULPROBE NANJING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no universal fluorescent dye labeling method that can solve the technical problems of specific labeling of fluorescent dyes when labeling actin and endogenous labeling of living cells. Fluorescence imaging of proteins, including ordinary fluorescence imaging and super-resolution microscopy imaging to further improve the resolution of optical imaging

Method used

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Examples

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Effect test

Embodiment 1

[0039] A fluorescent dye probe has the following structure:

[0040] MGVADLIKKFESISKEE-GGGGK-R 1 -GG-rRrRrRRR

[0041] Among them, MGVADLIKKFESISKEE is the amino acid sequence of the actin recognition group; GGGGK is the first linking group, and the fluorescent dye group is Rhodamine B, R 1 structured as GG is the second linking group; rRrRrRRR is the amino acid sequence of the cell penetrating peptide, r is D-arginine, and R is L-arginine.

[0042] The fluorescent dye probe is synthesized by Shanghai Aobo Biotechnology Co., Ltd., and its synthesis method adopts the solid-phase peptide synthesis method.

Embodiment 2

[0044] A fluorescent dye probe has the following structure:

[0045] MGVADLIKKFESISKEE-GGGGK-R 1 -GG-rRrRrRRR

[0046] Among them, MGVADLIKKFESISKEE is the amino acid sequence of the actin recognition group; GGGGK is the first linking group, the fluorescent dye group is fluorescein isothiocyanate, R 1 structured as GG is the second linking group; rRrRrRRR is the amino acid sequence of the cell penetrating peptide, r is D-arginine, and R is L-arginine.

[0047] The fluorescent dye probe is synthesized by Shanghai Aobo Biotechnology Co., Ltd., and its synthesis method adopts the solid-phase peptide synthesis method.

Embodiment 3

[0049] A fluorescent dye probe has the following structure:

[0050] MGVADLIKKFESISKEE-GGGGK-R 1 -GG-rRrRrRRR

[0051] Among them, MGVADLIKKFESISKEE is the amino acid sequence of the actin recognition group; GGGGK is the first linking group, and the fluorescent dye group is the photoswitch Rhodamine B, R 1 structured as GG is the second linking group; rRrRrRRR is the amino acid sequence of the cell penetrating peptide, r is D-arginine, and R is L-arginine.

[0052] The fluorescent dye probe is synthesized by Shanghai Aobo Biotechnology Co., Ltd., and its synthesis method adopts the solid-phase peptide synthesis method.

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Abstract

A fluorochrome probe is disclosed. The structure of the fluorochrome probe is shown as a formula (I) that is MGVADLIKKFESISKEE-GGGGK-R1-GG-rRrRrRRR, wherein the MGVADLIKKFESISKEE is an amino acid sequence of an actin recognition group, the GGGGK is a first connecting group, the R1 is a fluorochrome group, the GG is a second connecting group, the rRrRrRRR is an amino acid sequence of a cell permeable peptide, the r is D-arginine, and the R is L-arginine. The fluorochrome probe can specifically label living-cell endogenic actin, and obtain super-resolution microimaging with a higher resolution after fluorescence imaging of the living-cell endogenic actin is achieved.

Description

technical field [0001] The invention belongs to the field of microscopic imaging, and more specifically relates to a fluorescent dye probe. Background technique [0002] There are currently two forms of fluorescent labeling of actin in living cells, fluorescent protein labeling and fluorescent dye labeling. Among them, fluorescent proteins are the most widely used. Actin labeled with fluorescent dyes needs to overcome the problems of specific labeling and endogenous labeling in living cells, and few labeling methods have been successfully used so far. However, fluorescent dyes generally have higher brightness, better anti-bleaching ability and photostability, and have a wider emission spectrum coverage area, which can easily meet the general fluorescence imaging of various colors. Not only that, in the field where ordinary optical imaging cannot break through the resolution limit, that is, in the field of super-resolution microscopy imaging, fluorescent dyes have more obvi...

Claims

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Application Information

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IPC IPC(8): C09K11/06C07K19/00G01N21/64
Inventor 张玉慧骆清铭潘登樊锋凯
Owner SINGULPROBE NANJING BIOTECH CO LTD
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