A fluorochrome probe

Abstract

A fluorochrome probe is disclosed. The structure of the fluorochrome probe is shown as a formula (I) that is MGVADLIKKFESISKEE-GGGGK-R1-GG-rRrRrRRR, wherein the MGVADLIKKFESISKEE is an amino acid sequence of an actin recognition group, the GGGGK is a first connecting group, the R1 is a fluorochrome group, the GG is a second connecting group, the rRrRrRRR is an amino acid sequence of a cell permeable peptide, the r is D-arginine, and the R is L-arginine. The fluorochrome probe can specifically label living-cell endogenic actin, and obtain super-resolution microimaging with a higher resolution after fluorescence imaging of the living-cell endogenic actin is achieved.

Description

technical field [0001] The invention belongs to the field of microscopic imaging, and more specifically relates to a fluorescent dye probe. Background technique [0002] There are currently two forms of fluorescent labeling of actin in living cells, fluorescent protein labeling and fluorescent dye labeling. Among them, fluorescent proteins are the most widely used. Actin labeled with fluorescent dyes needs to overcome the problems of specific labeling and endogenous labeling in living cells, and few labeling methods have been successfully used so far. However, fluorescent dyes generally have higher brightness, better anti-bleaching ability and photostability, and have a wider emission spectrum coverage area, which can easily meet the general fluorescence imaging of various colors. Not only that, in the field where ordinary optical imaging cannot break through the resolution limit, that is, in the field of super-resolution microscopy imaging, fluorescent dyes have more obvi...

AI Technical Summary

Problems solved by technology

[0004] At present, there is no universal fluorescent dye labeling method that can solve the technical problems of specific labeling of fluorescent dyes when labeling actin and endogenous lab

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