The invention discloses a high-manganese nitrogen-free high-strength and high-toughness anti-hydrogen embrittlement austenitic stainless steel and a preparation method thereof. The austenitic stainless steel comprises the following chemical components of less than 0.05% of C, less than 0.50% of Si, 6%-10% of Mn, 12%-16% of Cr, 4.5%-6.5% of Ni, 0.5% -3% of Mo, less than 0.01% of S, less than 0.03%of P, and the balance Fe and trace added Nb, Ti, V, Al, Cu and inevitable impurity elements. The manufacturing method comprises the steps of smelting (casting), forging (hot rolling), rolling, tempering and the like. The high-strength and high-toughness stainless steel prepared by the preparation method disclosed by the invention is characterized in that the high-strength and high-toughness stainless steel is based on 13Cr-5Ni-2Mo / 15Cr-6Ni-2Mo super martensite stainless steel, the austenitic stainless steel is subjected to austenitizing by adding cheap Mn elements, the high strength is achieved through the residual martensite back stress strengthening and the fine crystal strengthening of an austenite matrix, the high toughness is obtained through phase change, and the H2S (hydrogen embrittlement) stress corrosion resistance of the material is improved through the austenitizing. The prepared high-strength steel has extremely high toughness and anti-hydrogen embrittlement performancewhile maintaining good corrosion resistance. The method disclosed by the invention is simple in process, easy to realize industrialization, and capable of being used for an oil pipe containing H2S oil(gas) and an environment with high corrosion resistance and anti-hydrogen embrittlement requirements.