REMAP marker for evaluating genetic diversity of cyprinus carpio population

A marker, carp technology, applied in the direction of DNA/RNA fragment, recombinant DNA technology, microbial determination/inspection, etc., can solve the problem of few fish research and so on

Active Publication Date: 2015-04-08
CHINESE ACAD OF FISHERY SCI
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, REMAP technology is only common i...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • REMAP marker for evaluating genetic diversity of cyprinus carpio population
  • REMAP marker for evaluating genetic diversity of cyprinus carpio population
  • REMAP marker for evaluating genetic diversity of cyprinus carpio population

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] 1. Acquisition of carp genome sequence

[0019] The carp genome was sequenced using a 454 high-throughput sequencer to obtain 0.01X coverage and a sequencing depth of 1.

[0020] 2. Identification process of carp LTR sequence

[0021] Identification of transposon taxa from fragment sequences in the carp genome:

[0022] Use the RepeatModeler (http: / / www.repeatmasker.org / RepeatModeler.html) of the Repeat masker software to perform denovo repeat sequence identification;

[0023] Identify sequences containing retrotransposons:

[0024] Use blastn to confirm the presence of transport-related proteins in TE: blastx alignment of these sequences with the amino acid sequences of zebrafish retrotransposons (http: / / www.repeatmasker.org / RepeatProteinMask.html#database). Sort all translocation-related encoded proteins by length;

[0025] Identification of full-length retrotransposon sequences:

[0026] Use the software LTR FINDER to scan candidate sequences, and select sequenc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a cyprinus carpio retrotransposon marker. A high-polymorphism retrotransposon REMAP marker of cyprinus carpio is developed by predicting and identifying a cyprinus carpio retrotransposon sequence, screening a cyprinus carpio polymorphism REMAP marker and performing genetic polymorphism detection on a cyprinus carpio retrotransposon locus. The cyprinus carpio retrotransposon marker disclosed by the invention can be applied to the research on the population genetic structure, genetic variation level and the like of the cyprinus carpio population and the germplasm identification of the geographical population of cyprinus carpio.

Description

technical field [0001] The invention relates to a molecular marker technology, in particular to a REMAP polymorphic marker for assessing genetic diversity and germplasm identification in carp geographic populations. Background technique [0002] Carp (Cyprinus Carpio) is one of the most important freshwater aquaculture species in my country. According to the FAO2010 report, the total output of carp accounts for 10% of the world's fish meat output. Because carp is widely distributed in major water systems in my country, and due to artificial selection and breeding, genetic diversity of some fine carp species has experienced a bottleneck effect, and the genetic diversity has declined sharply. Therefore, it is very necessary to protect and identify carp germplasm resources. [0003] REMAP technology is a polymorphic molecular marker newly developed in recent years. It is widely used because of its wide distribution in the genome, high polymorphism, and high identification effic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/11C12Q1/68
Inventor 张研徐鹏李晓敏孙效文
Owner CHINESE ACAD OF FISHERY SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap