LED light source based anthurium andraeanum tissue culture method

An LED light source and tissue culture technology, applied in the field of plant tissue culture, can solve the problems of long culture period and low reproduction coefficient, and achieve the effects of strong environmental adaptability, high survival rate and simple operation

Inactive Publication Date: 2015-04-22
NANJING QISEGUANG AGRI SCI & TECH CO LTD
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  • Abstract
  • Description
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Problems solved by technology

Although the application of tissue culture has greatly reduced the production cost of anthurium seedlings, there are still outstanding problems such as low reproduction coefficient and long training period in the tissue culture and rapid propagation of anthurium.

Method used

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  • LED light source based anthurium andraeanum tissue culture method

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Embodiment Construction

[0013] The present invention will be specifically introduced below in conjunction with specific embodiments, and the methods used are conventional methods unless otherwise specified.

[0014] An anthurium tissue culture method based on LED light source:

[0015] (1) Selection and disinfection of explants: select the fully expanded young leaves of the anthurium (preserving the midrib), first put them in 75% ethanol for 30s, wash 5 times with sterile water, and then sterilize them in 5% sodium hypochlorite for 15 minutes. Wash the bacteria with water for 5 times and absorb the water with sterile absorbent paper;

[0016] (2) Induction of callus: take the sterilized leaves and cut them into 1 cm 2 Square, inoculate in callus induction medium, inoculate 4 leaf explants in each petri dish, seal with Parafilm, and cultivate in the dark;

[0017] (3) Adventitious bud differentiation of callus: inoculate the callus induced by leaves in a petri dish, and inoculate about 0.3g callus per bottle,...

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Abstract

Anthurium andraeanum is a perennial evergreen herb plant, belongs to the Anthurium genus, the Anthurieae tribe, and ranks the second only to tropical orchids in the global tropical flowers. The present invention relates to a LED light source based anthurium andraeanum callus adventitious bud differentiation method which belongs to the technical field of plant tissue culture. The tissue culture method uses LED light sources with different light qualities to treat the Anthurium andraeanum callus during the periods of adventitious bud differentiation, radication and strong seedlings cultivation, and acclimatization and transplantation in order to shorten the rapid propagation time of the anthurium andraeanum tissue culture and obtain high-quality anthurium andraeanum seedlings.

Description

Technical field [0001] The invention relates to an anthurium tissue culture method based on an LED light source, and belongs to the technical field of plant tissue culture. Background technique [0002] Anthurium, also known as anthurium, is a perennial evergreen plant of the genus Anthurium of the Araceae family. The anthurium has gorgeous colors, tall stems, and beautiful green leaves. It is one of the world-famous cut flowers and potted flowers. The propagation methods of anthurium include cuttings, ramets, artificial seeds and tissue culture. However, because it is difficult to expand production by ramet propagation, sowing propagation requires artificial pollination to obtain seeds, so tissue culture is currently the main and effective method for the production of anthurium seedlings. Although the application of tissue culture greatly reduces the production cost of anthurium seedlings, there are still outstanding problems in the process of tissue culture and rapid propaga...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 崔瑾章锐
Owner NANJING QISEGUANG AGRI SCI & TECH CO LTD
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