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Artificial insemination liquid for acipenser dabryanus and preparation method thereof

A technology of Dabry's sturgeon and semen, which is applied in the field of artificial insemination and its preparation, can solve the problems of low fertilization rate and achieve the effect of vigor and life extension

Inactive Publication Date: 2015-05-13
FISHERIES INST SICHUAN ACADEMY OF AGRI SCI
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AI Technical Summary

Problems solved by technology

[0007] In view of the above technical problems, the present invention provides an artificial insemination solution suitable for Dabry's sturgeon, which solves the problem of low fertilization rate in the artificial breeding of Dabry's sturgeon at the present stage

Method used

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Embodiment Construction

[0013] The specific implementation of the present invention will be described in conjunction with the examples.

[0014] The male and female fish of Acipenser dabryii with mature selective gland development, and the female fish use LHRH-A 2 +Cyp pituitary gland, 2 injections, 12h interval between 2 injections, 1 injection for male fish. After the injection, the sturgeons were kept in running water until their gonads matured.

[0015] Sperm collection of Acipenser dabryi: For male Acipenser dabryii with mature selective glands, dry the water around the genital pores and abdomen with a dry towel, use a hose to guide the sperm, and store the semen at 4°C after oxygenation.

[0016] Artificial egg collection of Dabry's sturgeon: after the second injection of the female fish, check the ovulation status of the female fish in the pool. For the female Dabry's sturgeon that has started to ovulate, wipe dry around the genital opening and abdomen with a dry towel, and gently squeeze the...

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PUM

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Abstract

The invention relates to artificial insemination liquid and a preparation method thereof, in particular to artificial insemination liquid for acipenser dabryanus and a preparation method thereof. The preparation method comprises the steps of dissolving NaCl, KCl, MgCl2.6H2O, CaCl2, glucose and Tris-base into deionized water to form a mixed solution, and adjusting the pH of the mixed solution by HCl to 7.8 so as to form the artificial insemination liquid for the acipenser dabryanus. Every 500 millimeters of the artificial insemination liquid contains 0.585g of NaCl, 0.0019 of KCl, 0.5083g of MgCl2.6H2O, 0.2775g of CaCl2, 5.4g of glucose and 1.2114g of Tris-base. The artificial insemination liquid for the acipenser dabryanus consists of different ions and macromolecular substances by completely aiming at substances for activating sperms of the acipenser dabryanus; the ions and the macromolecular substances can achieve a certain optimizing effect on the movement of the sperms; the activating rate of the sperms is over 90 percent, the activity of the sperms is obviously improved, and the service life of the sperms is obviously prolonged; the activity of the sperms can be improved from 103-123s to 172s-185s; the fertilization rate is increased from 13-29 percent to 54-58 percent.

Description

technical field [0001] The invention relates to an artificial insemination fluid and a preparation method thereof, in particular to an artificial insemination fluid and a preparation method thereof. Background technique [0002] Dabry's sturgeon, also known as Changjiang sturgeon and Shalazi, is a unique freshwater resident species of sturgeon in my country. It is distributed in the upper reaches of the Yangtze River and the lower reaches of the Jinsha River. In recent years, due to the impact of many human activities such as overfishing, water conservancy projects, shipping and water pollution, the wild population of Dabry's sturgeon has been decreasing day by day, and the population resources have declined seriously. The Dabry's sturgeon in the Yangtze River Basin is almost extinct. In order to protect this It is imperative to carry out artificial breeding research on Dabry's sturgeon and finally realize large-scale artificial breeding. [0003] Dabry's sturgeon grows fast ...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81A01K61/00
Inventor 陈春娜黄颖颖陈先均周波何斌李孟均陈彦伶肖宇龚全杜军刘亚李军林珏赖建生赵刚
Owner FISHERIES INST SICHUAN ACADEMY OF AGRI SCI
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