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Rapid molecular identification method for cinnamon

A cinnamon, rapid technology, applied in the field of identification of cinnamon with specific SNP, can solve the problem of mixed use, and achieve the effect of accurate identification and wide applicability

Inactive Publication Date: 2015-05-13
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cinnamon, a plant in the Lauraceae family, is the only source of cinnamon medicinal materials in the Pharmacopoeia. In recent years, it has often appeared in the market that C. Similar, easy to cause confusion

Method used

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  • Rapid molecular identification method for cinnamon
  • Rapid molecular identification method for cinnamon
  • Rapid molecular identification method for cinnamon

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1) 27 samples of cinnamon and 37 samples of other species were collected from different origins, and 35 mg of medicinal materials were taken respectively, ground on a MM400 ball mill (Retsch, Germany), and extracted with a plant genomic DNA extraction kit (Tiangen Biotech Co., China) total DNA.

[0018] Table 1 Samples of cinnamon and its relatives and counterfeit products

[0019]

[0020]

[0021]

[0022] 2) PCR amplification

[0023] The primer sequence is psbA: 5′-GTTATGCATGAACGTAATGCTC-3′, trnH: 5′-CGCGCATGGTGGATTCACAATCC-3′ and was synthesized by Sangon Bioengineering Co., Ltd. (Beijing). Primers were dissolved in sterile deionized and diluted to 2 μmol / μL.

[0024] 25μL reaction system: PCR Buffer (10×) 2.5μL, Mg 2+ 2 μL (25 mmol / L), 2 μL (2.5 mmol / L) of dNTPs mixture, 1.0 μL (2.5 μmol / L) of each primer, 1 μL (~30ng) of template DNA, 1.0 U of Taq DNA polymerase, add sterilized double distilled water to 25 μL for PCR amplification.

[0025] PCR reac...

Embodiment 2

[0034] Identification of embodiment 2 cinnamon decoction pieces

[0035] Basically the same as Example 1, the difference is that cinnamon decoction pieces are used as samples, DNA is extracted and identified, and the result can also specifically detect the above-mentioned 1 SNP site, and sequence comparison shows that the psbA-trnH of cinnamon decoction pieces DNA The 80th position of the intergenic region sequence (SEQ ID No.1) is T.

Embodiment 3

[0036] The identification of embodiment 3 cinnamon powder

[0037] Basically the same as Example 1, the difference is that cinnamon powder is used as a sample, DNA is extracted and identified, and the result can also specifically detect the above-mentioned 1 SNP site, and sequence comparison shows that psbA-trnH of cinnamon powder DNA The 80th position of the intergenic region sequence (SEQ ID No.1) is T.

[0038]

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PUM

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Abstract

The invention discloses a rapid cinnamon identification method which comprises the following steps: (1) by taking to-be-detected sample DNA as a template, amplifying a sequence shown as SEQ ID No.1; (2) sequencing the amplification product, analyzing the sequence shown as SEQ ID No.1, identifying the product to be cinnamon if the 80th site from the 5' end of the sequence shown as SEQ ID No.1 is T, and identifying the product to be not cinnamon if the 80th site from the 5' end of the sequence shown as SEQ ID No.1 is C. According to the method disclosed by the invention, the original cinnamon plants and sibling species thereof, medicinal materials, raw medicinal slices, powder and adulterants can be rapidly and accurately identified.

Description

technical field [0001] The invention belongs to the technical field of identification of source varieties of Chinese medicinal materials, and in particular relates to a method for identifying cassia cinnamon with specific SNPs. Background technique [0002] Cinnamon Cinnamomi Cortex is the dry bark of Cinnamomum cassia Presl. The twigs of cinnamon (Cinnamon twigs) are also used for medicinal purposes, and are recorded in the 2010 edition of the Pharmacopoeia of the People's Republic of China. Cinnamon has been listed as the top grade in herbal medicines of all dynasties, and it is believed that it has the functions of nourishing fire and yang, drawing fire back to its source, dispelling cold and relieving pain, promoting blood circulation and stimulating menstruation. In 2002, the Ministry of Health of the People's Republic of China announced the "Notice on Further Regulating the Management of Health Food Raw Materials", which made specific regulations on medicine and food ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 姚辉杨培韩建萍陈士林宋经元
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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