Dietary supplement with bacteriostatic action and preparation method and application thereof

A dietary supplement and a technology of antibacterial effect, applied in the field of dietary supplements and their preparation, can solve the problems of unclear antibacterial effect, complicated preparation process, and lack of natural sources, and achieve high food safety, simple process, and application promising effect

Active Publication Date: 2015-07-08
BRIGHT DAIRY & FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Therefore, in order to solve the problems that current dietary supplements with bacteriostatic properties lack natural sources, and the preparation process of such dietary supplements is...

Method used

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  • Dietary supplement with bacteriostatic action and preparation method and application thereof
  • Dietary supplement with bacteriostatic action and preparation method and application thereof
  • Dietary supplement with bacteriostatic action and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of embodiment 1 dietary supplement and the detection of antibacterial effect

[0026] 1. Materials and methods

[0027] Preparation of seeds (fermentation strain): Paenibacillus sp. (Paenibacillus sp.) (the preservation number of Paenibacillus is CGMCC No.8333, and the strain is named DSM28815. For the source of the strain, please refer to the publication number CN 103740618A) of the lyophilized powder was dissolved in a small amount of sterile distilled water, and a loop was used to draw a line on the TYC solid medium (purchased from OXOID Co., UK) with an inoculation loop. Pick a single colony and put it into 10mL TYC liquid medium (purchased from OXOID Co., UK), use a vortex shaker to evenly disperse the colony in the liquid medium, culture it at 30°C and 180rpm for 24 hours, and take it out with 2% (volume Percentage) inoculum amount was inoculated in TYC liquid medium (purchased from OXOID Co., UK), 30°C, 180rpm shaking culture for 24h, the culture...

Embodiment 2

[0040] The preparation of embodiment 2 dietary supplements and the detection of antibacterial effect

[0041] 1. Materials and methods:

[0042] Preparation of tomato juice medium: ripe tomatoes were washed, peeled, squeezed by a juicer, filtered through 100-mesh gauze to extract the juice, boiled for 10 minutes, centrifuged at 4000g for 10 minutes to get the supernatant, adjusted to pH 8.0, sterilized at 135°C for 10 minutes, cooled to room temperature , to obtain sterile tomato juice medium.

[0043] Other materials and methods are the same as in Example 1.

[0044] 2. Preparation of dietary supplements

[0045] Paenibacillus sp. was inoculated in a tomato juice medium with pH 8.0 at an inoculum of 1% (volume percentage), cultured with shaking at 37°C and 100 rpm for 72 hours to obtain a fermented liquid, and the fermented liquid was centrifuged at 10,000 g for 8 min to obtain The supernatant was freeze-dried to obtain dietary supplement B.

[0046] 3. Determination of a...

Embodiment 3

[0051] Preparation of embodiment 3 dietary supplement and detection of antibacterial effect

[0052] 1. Materials and methods are the same as in Example 1.

[0053] Preparation of tomato juice medium: ripe tomatoes were washed, peeled, squeezed by a juicer, filtered through 100-mesh gauze to extract the juice, boiled for 5 minutes, centrifuged at 8000g for 10 minutes to get the supernatant, adjusted to pH 7.0, sterilized at 121°C for 20 minutes, cooled to room temperature , to obtain sterile tomato juice medium.

[0054] Other materials and methods are the same as in Example 1.

[0055] 2. Preparation of dietary supplements

[0056] Paenibacillus sp. was inoculated into a tomato juice medium with pH 7.0 at an inoculum size of 2% (volume percentage), cultured at 30°C and 180rpm with shaking for 48 hours to obtain a fermented liquid, and the fermented liquid was centrifuged at 12,000 g for 5 min to obtain The supernatant was freeze-dried to obtain dietary supplement C.

[00...

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Abstract

The invention discloses a dietary supplement with a bacteriostatic action and a preparation method and application thereof. The method for preparing the dietary supplement with the bacteriostatic action comprises the following steps: (1) inoculating Paenibacillus (Paenibacillus sp.) in a tomato juice culture medium, shaking and performing fermentation culturing, thereby obtaining the fermentation liquor; and (2) centrifuging the fermentation liquor obtained in the step (1), taking the supernatant, and performing freeze drying, thereby obtaining the product. According to the dietary supplement with the bacteriostatic action disclosed by the invention, the adopted raw material refer to tomato juice during preparation, and the source is natural, so the dietary supplement has high food safety. Moreover, the preparation method of the dietary supplement is simple in process, and only three steps, namely fermenting, centrifuging and freeze drying are needed so as to finish the method, and the possible pollution problem brought by the traditional complicated process is greatly reduced. The obtained dietary supplement is obvious in bacteriostatic effect and stable in bacteriostatic action.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a dietary supplement with antibacterial effect and its preparation method and application. Background technique [0002] A dietary supplement is a daily nutritional supplement that can improve the health of the body and reduce the risk of disease through the supplement of vitamins, minerals, amino acids and other nutrients. However, the existing dietary supplements are basically prepared through the combination of various nutritional ingredients, and the complex formula components greatly increase the risk of contamination of the dietary supplements during the preparation process, which in turn poses a threat to the health of consumers. potential threat. In addition, since the probiotic effect of microbial metabolites on the body has been widely recognized, it is bound to complement each other when used in conjunction with dietary supplements. However, there are currentl...

Claims

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Application Information

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IPC IPC(8): A23L1/29A23L33/00
Inventor 韩瑨郭本恒刘振民鄢明辉徐晓芬
Owner BRIGHT DAIRY & FOOD
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