N.braziliensis selective medium for pleural effusion sample

A technology for selecting medium and Nocardia, which is applied in the field of Nocardia brasiliensis selective medium, can solve the problems of slow growth, low positive rate, and lesion transfer, etc., and achieves the effect of high separation rate and reliable detection.

Inactive Publication Date: 2015-08-12
辛本勤
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The primary culture of Nocardia brasiliensis grows slowly. Pleural effusion specimens are easily covered by Candida albicans, Staphylococcus aureus and other fungi and miscellaneous bacteria on blood agar medium. Nocardia brasiliensis cannot grow, and the positive rate is low. , 60% of Nocardia brasiliensis was missed by using the traditional method of laboratory culture for 48 hours, and it took about 1 week to make a more accurate identification after more than 96 hours of culture, and the biochemical identification required 4 to 6 weeks. Delayed time, causing multiple lesions to metastasize and increasing the fatality rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. The formula for preparing 1000ml medium contains: beef liver powder 15.0g; malt extract powder 2.0g; yeast extract powder 5.0g; sodium chloride 5.0g; agar 15.0g; ferrous lactate 0.01g; zinc gluconate Calcium 0.05g; Nalidixic acid 0.05g; Trichodone 0.001g; Asparagine 0.1g; Aseptic defibrinated sheep blood 100ml; Distilled water is added to 1000ml. Preparation method Weigh beef liver powder; malt extract powder; yeast extract powder; sodium chloride; agar; ferrous lactate; zinc calcium gluconate; trichodone; asparagine and dissolve in distilled water, 280°C, sterilize for 15 minutes, Cool to 45°C, add sterilized nalidixic acid; mix the sterile defiberized sheep blood, dilute the sterilized distilled water to 1000ml, and dispense.

Embodiment 2

[0029] Example 2. The formula for preparing 1000ml culture medium contains: beef liver powder 10.0g; malt extract powder 5.0g; yeast extract powder 8.0g; sodium chloride 5.0g; agar 15.0g; ferrous lactate 0.01g; zinc gluconate Calcium 0.03g; Nalidixic acid 0.02g; Trichodone 0.003g; Asparagine 0.01g; Complex protein phosphopeptide 0.1g; Sterile defibrinated sheep blood 80ml; Distilled water is added to 1000ml. Preparation method Weigh bovine liver powder; malt extract powder; yeast extract powder; sodium chloride; agar; ferrous lactate; calcium zinc gluconate; trichodone; asparagine mixed, dissolved in distilled water, 280℃, sterilized for 15min , Cool to 45°C, add sterile nalidixic acid; sterile complex protein phosphopeptide; sterile defibrinated sheep blood, mix well, dilute sterilized distilled water to 1000ml, and then dispense.

Embodiment 3

[0030] Example 3, the formula for preparing 1000ml medium contains: beef liver powder 15.0g; malt extract powder 3.0g; yeast extract powder 10.0g; sodium chloride 5.0g; agar 20.0g; ferrous lactate 0.02g; zinc gluconate Calcium 0.05g; Nalidixic acid 0.05; Trichodone 0.005g; Asparagine 0.05g; Vitamin K 1 0.01g; 90ml sterile defibrinated sheep blood; add distilled water to 1000ml. Preparation method Weigh beef liver powder; malt extract powder; yeast extract powder; sodium chloride; agar; ferrous lactate; zinc calcium gluconate; trichodone; asparagine mixed, dissolved in distilled water, 280°C, sterilized for 15 minutes , Cool to 45℃, add sterilized nalidixic acid; sterilized vitamin K 1 ; Sterile defibrated sheep blood is mixed, and the volume of sterilized distilled water is adjusted to 1000ml and then aliquoted.

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Abstract

The invention discloses an N.braziliensis selective medium for a pleural effusion sample and belongs to the field of testing microculture. The N.braziliensis selective medium is characterized in that 1000ml N.braziliensis selective medium is prepared from beef liver powder, malt extract powder, yeast extract powder, sodium chloride, agar, iron lactate, calcium and zinc gluconate, nalidixic acid, argutone, asparagine and sterile de-fibering sheep blood by adding distilled water to 1000ml. Compared with the prior art, the N.braziliensis selective medium has the advantages of testing reliability and high N.braziliensis isolation rate.

Description

Technical field [0001] The invention relates to the field of testing microorganism cultivation, in particular to a selection medium of Nocardia brasiliensis for pleural effusion specimens. Background technique [0002] Microbial culture is a technology that uses artificial methods to grow and reproduce microorganisms, and selective media is a medium designed to promote or inhibit certain types of organisms (such as cells or bacteria). Separate the required microorganisms from the mixed microorganisms. Nocardia braziliensis (N. braziliensis) is an aerobic soil saprophytic bacteria belonging to the genus Nocardia. It belongs to the phylum Actinomycota. The bacteria are widely found in air, dust, soil and livestock surfaces. Nocardia braziliensis is toxic Strong and pathogenic, it can cause outbreaks, mostly through the respiratory system or primary infections, causing chronic purulent infections in subcutaneous tissues, lungs, and brain. Nocardia brasiliensis invades the subcutan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
Inventor 辛本勤
Owner 辛本勤
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