Composite microbial bacterial agent and its preparation method and application
A composite microbial inoculant and microorganism technology, which is applied in drilling compositions, chemical instruments and methods, and earth-moving drilling, etc. It can prevent oil chromatography wax, remove wax blockage, and improve fluidity.
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Embodiment 1
[0042] The preparation of embodiment 1 composite microbial bacterial agent
[0043] (1) Fermenting and culturing the thermophilic and halotolerant facultative anaerobic bacillus with the microorganism preservation number: CGMCC NO.6570 and the thermophilic urea bacillus with the microorganism preservation number: CGMCC NO.5818, respectively preparing bacterial liquids, namely Obtain thermophilic halotolerant facultative anaerobic bacillus bacterial agent and thermophilic urea bacillus bacterial agent;
[0044] In terms of mass percentage, each component in the medium for fermentation and cultivation of the thermophilic halotolerant facultative anaerobic bacillus and thermophilic urea bacillus is: liquid paraffin 2%, KH 2 PO 4 0.05%, Na 2 HPO 4 0.1%, (NH 4 ) 2 SO 4 0.2%, MgSO 4 ·7H 2 O 0.02%, CaCl 2 2H 2 O 0.001%, FeCl 3 0.01%, corn steep liquor dry powder 0.3%, trace element solution 0.2%, multivitamin solution 0.2%, and the balance is water; among them, trace eleme...
Embodiment 2
[0049] The preparation of embodiment 2 composite microbial bacterial agents
[0050] According to the method of Example 1, the composite microbial bacterial agent is prepared, the difference is: according to the volume ratio, thermophilic salt-tolerant facultative anaerobic bacillus bacterial agent: thermophilic urea bacterial agent: biosurfactant = 1:1:0.01 , that is.
Embodiment 3
[0051] The preparation of embodiment 3 composite microbial bacterial agents
[0052] According to the method of Example 1, the composite microbial bacterial agent is prepared, the difference is: according to the volume ratio, thermophilic salt-tolerant facultative anaerobic bacillus bacterial agent: thermophilic urea bacterial bacterial agent: biosurfactant = 3: 3: 0.5 , that is.
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