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Preparation method of acquired immune deficiency syndrome antigen detection chip

An antigen detection and AIDS technology, applied in the field of AIDS antigen detection chip preparation, can solve the problems of limited detection sensitivity, difficult to achieve high-throughput rapid detection of patient groups, and large consumption of reagents, achieving high sensitivity and rapid detection, and saving raw materials. And manpower, material resources and time, improve the effect of detection signal and detection sensitivity

Inactive Publication Date: 2015-10-14
WORMHOLE BEIJING HEALTH TECH CO LTD
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Problems solved by technology

These methods can realize the detection of HIV p24 antigen to a certain extent, but there are obvious shortcomings: one is that the detection sensitivity is limited because the markers used are traditional markers; Therefore, high-throughput quantification cannot be achieved, and it is difficult to achieve high-throughput rapid detection of a large number of patient populations, such as HIV screening, monitoring, and blood donation screening for patient populations in areas with severe epidemics. , and consumes a lot of reagents

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  • Preparation method of acquired immune deficiency syndrome antigen detection chip

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Embodiment Construction

[0017] The present invention will be further described below:

[0018] Such as figure 1 Shown, the present invention comprises the following steps:

[0019] Step 1. Synthesize novel core-shell silica fluorescent nanoparticles. The synthetic raw materials include surfactant, co-surfactant, cyclohexane and silver nitrate aqueous solution. The synthetic raw materials are mixed into a microemulsion, adding a reducing agent, and the Ag + It is reduced to elemental Ag, and then added tetraethyl orthosilicate and ammonia water to cause a polymerization reaction, and a layer of SiO is coated on the surface of nano-silver. 2 , adding a reagent with a chelating group ligand, separating the nanoparticles after the reaction, and then dispersing the nanoparticles into the Eu 3+ or Eu 3+ with Tb 3+ Fully react in aqueous solution, centrifuge, wash and dry the nanoparticles to obtain the final required nanoparticles. The core of the nanoparticles is Ag core and the shell is SiO 2 , the ...

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Abstract

The invention discloses a preparation method of an acquired immune deficiency syndrome antigen detection chip. The preparation method comprises the following steps: step one, synthesizing novel core-shell silicon dioxide fluorescence nanoparticles; step two, marking streptavidin; and step three, preparing a protein chip based on HIV p24 antigen detection. According to the preparation method, according to the antigen-antibody high specific immunity reaction, the detection signal and the detection sensitivity are improved by adopting rare earth fluorescence nanoparticles as markers, and a micro-array protein chip is prepared on the basis, so that few samples are needed, a great number of patients can be detected in a high throughout, high sensitivity and rapidness manner within an extremely short time, raw materials, manpower, material resources and time aregreatly saved.

Description

technical field [0001] The invention relates to a preparation method, in particular to a preparation method of an AIDS antigen detection chip. Background technique [0002] Currently, there are two types of AIDS diagnostic methods: virus detection and antibody detection. Virus detection is an important method for diagnosing AIDS. Because HIV-infected people first appear in the body is HIV, and only after a period of time will they produce antibodies in the body. Therefore, virus detection is an effective method for early diagnosis of HIV. Virus detection includes cell culture (virus isolation), antigen detection and viral nucleic acid detection. Among them, the method of cell culture has strong specificity, but because a certain number of infected cells must exist to culture and isolate the virus, the sensitivity is poor, and the operation time is long and complicated, so it is not suitable for rapid clinical detection. The nucleic acid sequence amplification reaction can ...

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/54346
Inventor 戴天岩
Owner WORMHOLE BEIJING HEALTH TECH CO LTD
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