Preparation method of acquired immune deficiency syndrome antigen detection chip

Abstract

The invention discloses a preparation method of an acquired immune deficiency syndrome antigen detection chip. The preparation method comprises the following steps: step one, synthesizing novel core-shell silicon dioxide fluorescence nanoparticles; step two, marking streptavidin; and step three, preparing a protein chip based on HIV p24 antigen detection. According to the preparation method, according to the antigen-antibody high specific immunity reaction, the detection signal and the detection sensitivity are improved by adopting rare earth fluorescence nanoparticles as markers, and a micro-array protein chip is prepared on the basis, so that few samples are needed, a great number of patients can be detected in a high throughout, high sensitivity and rapidness manner within an extremely short time, raw materials, manpower, material resources and time aregreatly saved.

Description

technical field

[0001] The invention relates to a preparation method, in particular to a preparation method of an AIDS antigen detection chip. Background technique

[0002] Currently, there are two types of AIDS diagnostic methods: virus detection and antibody detection. Virus detection is an important method for diagnosing AIDS. Because HIV-infected people first appear in the body is HIV, and only after a period of time will they produce antibodies in the body. Therefore, virus detection is an effective method for early diagnosis of HIV. Virus detection includes cell culture (virus isolation), antigen detection and viral nucleic acid detection. Among them, the method of cell culture has strong specificity, but because a certain number of infected cells must exist to culture and isolate the virus, the sensitivity is poor, and the operation time is long and complicated, so it is not suitable for rapid clinical detection. The nucleic acid sequence amplification reaction can ...

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