Combined culture medium for in vitro regeneration of cucumber ovary
An in vitro regeneration and rooting medium technology, applied in the field of plant in vitro regeneration, can solve the problems of low regeneration frequency, large genotype constraints, and heavy workload of regenerated plants, so as to save seed usage, reduce cultivation steps, and differentiate powerful effect
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Examples
Embodiment 1
[0054] Embodiment 1 The preparation of the combination culture medium of the present invention
[0055] Combined medium includes callus induction medium, callus proliferation and differentiation medium, adventitious shoot elongation medium, rooting medium,
[0056] Callus induction medium: take NB medium as the basic medium, add sucrose at a final concentration of 30g / L, agar at 7g / L, TDZ at 1.5mg / L, and NAA at 0.2mg / L;
[0057] Callus proliferation and differentiation medium: use MS medium as the basic medium, add sucrose at a final concentration of 30g / L, agar at 7g / L, 6-BA at 2.5mg / L, and NAA at 0.01mg / L;
[0058] Adventitious shoot elongation medium: use MS medium as the basic medium, add sucrose at a final concentration of 30g / L, agar at 7g / L, and 6-BA at 0.5mg / L;
[0059] The rooting medium is: taking MS medium as basic medium, adding sucrose with a final concentration of 30g / L, agar with 7g / L, and IBA with 0.05mg / L.
[0060] The preparation method is as follows:
[0...
Embodiment 2
[0088] Embodiment 2 The preparation of the combination culture medium of the present invention
[0089] Combined medium includes callus induction medium, callus proliferation and differentiation medium, adventitious shoot elongation medium, rooting medium,
[0090] Callus induction medium: take NB medium as the basic medium, add sucrose at a final concentration of 30g / L, agar at 5g / L, TDZ at 1.2mg / L, and NAA at 0.5mg / L;
[0091] Callus proliferation and differentiation medium: use MS medium as the basic medium, add sucrose at a final concentration of 30g / L, agar at 5g / L, 6-BA at 1.5mg / L, and NAA at 0.01mg / L;
[0092] Adventitious shoot elongation medium: use MS medium as the basic medium, add sucrose at a final concentration of 30g / L, agar at 5g / L, and 6-BA at 0.3mg / L;
[0093] The rooting medium is: taking MS medium as basic medium, adding sucrose with a final concentration of 30g / L, agar with 7g / L, and IBA with 0.05mg / L.
[0094] The preparation method is as follows:
[009...
Embodiment 3
[0109] Embodiment 3 The preparation of the combination culture medium of the present invention
[0110] Combined medium includes callus induction medium, callus proliferation and differentiation medium, adventitious shoot elongation medium, rooting medium,
[0111] Callus induction medium: take NB medium as the basic medium, add sucrose at a final concentration of 40g / L, agar at 4g / L, TDZ at 2.0mg / L, and NAA at 0.5mg / L;
[0112] Callus proliferation and differentiation medium: use MS medium as the basic medium, add sucrose at a final concentration of 30g / L, agar at 4g / L, 6-BA at 3.0mg / L, and NAA at 0.1mg / L;
[0113] Adventitious shoot elongation medium: use MS medium as the basic medium, add sucrose at a final concentration of 30g / L, agar at 5g / L, and 6-BA at 0.1mg / L;
[0114] The rooting medium is: taking MS medium as basic medium, adding sucrose with a final concentration of 30g / L, agar with 7g / L, and IBA with 0.05mg / L.
[0115] The preparation method is as follows:
[01...
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Description
Claims
Application Information
- IPC
- C12N5/04; A01H4/00
- Inventors
- 杨宏; 李跃建