Multiplex Ligation Probe Amplification Detection Kit, Primers and Probes for Simultaneous Detection of Five Bovine Disease Viruses

Abstract

The invention discloses a multiple-connection probe amplification detection kit, primer and probe for simultaneously detecting the bluetongue viruses, the infectious bovine rhinotracheitis viruses, the bovine viral diarrhea viruses, the enzootic bovine leucosis viruses and the foot and mouth disease viruses. The multiple-connection probe is shown in sequence tables from SEQ ID NO:1 to SEQ ID NO:10. The primer is shown in sequence tables from SEQ ID NO:11 to SEQ ID NO:12. The primer, the probe and / or the multiple-connection probe amplification detection kit including the primer and the probe can detect the five vital cow disease pathogenies including the bluetongue viruses, the infectious bovine rhinotracheitis viruses, the bovine viral diarrhea viruses, the enzootic bovine leucosis viruses and the foot and mouth disease viruses at the same time, the detection time and cost are saved, and epidemic diseases can be diagnosed in time.

Description

technical field [0001] The invention provides a multiple connection probe amplification detection kit and primers and probes for detecting bluetongue, bovine viral diarrhea, bovine endemic leukemia, bovine infectious rhinotracheitis, and foot-and-mouth disease viruses. The purpose of realizing one sampling, one analysis and simultaneous detection of five kinds of cattle diseases belongs to the field of inspection and quarantine. Background technique [0002] Bluetongue virus (BTV), bovine viral diarrhea virus (BVDV), bovine enzootic leukemia virus (EBLV), infectious bovine rhinotracheitis virus (IBRV), foot-and-mouth disease virus (FMDV) The main common pathogens of disorders and digestive tract diseases are the most serious pathogens that harm the cattle industry. They are defined as cross-border pathogens by the World Organization for Animal Health (OIE) and are also important quarantine objects in the international trade of animals and animal products in my country. . [...

AI Technical Summary

Problems solved by technology

However, most of the currently established methods are single-target detection for one pathogen. In the actual detection, it needs to be repeated many times to complete the purpose of detecting different pathogens. The detection workload is large and the time is long, which cannot meet the rapid clearance of large-scale animal quarantine. the actual needs of

And it is difficult to realize the differential diagnosis of mixed infections and diseases with similar symptoms in actual samples

While establishing single-target pathogen detection technology, veterinary institutions in various countries have also successively developed multiplex PCR and multiplex fluorescent PCR that can simultaneously detect common cattle viruses. Fluorescence PCR is due to the mutual interference of the fluorescence emitted by the fluorescent groups used by different probes, and the limitation of fluorescence PCR instrument to the fluorescence resolution of different wavelengths also limits the development of fluorescence PCR multiple detection technology

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