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Method for constructing acetamiprid detection photoelectrochemical sensor and detecting method

A photoelectrochemical and construction method technology, which is applied in the direction of analyzing materials through chemical reactions, can solve problems such as low sensitivity, general selectivity, and complicated methods, and achieve the effects of improving sensitivity, simple equipment, and improving selectivity

Active Publication Date: 2015-10-28
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, each method has one or more of the following disadvantages: expensive equipment, complicated method, poor stability, time-consuming, low sensitivity, general selectivity, etc.
However, there is no report on the photoelectrochemical detection method of acetamiprid in the prior art, and the photoelectrochemical detection method of acetamiprid is still a blank in the current technical level.

Method used

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  • Method for constructing acetamiprid detection photoelectrochemical sensor and detecting method
  • Method for constructing acetamiprid detection photoelectrochemical sensor and detecting method
  • Method for constructing acetamiprid detection photoelectrochemical sensor and detecting method

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Effect test

Embodiment 1

[0034] Embodiment 1: a kind of construction method for the photoelectrochemical sensor that acetamiprid detects, comprises the steps:

[0035] (1) First mix 50ml 1mol·L -1 NaOH solution was added dropwise to 100ml of Co 2+ Zn(Ac) with a molar ratio of 1% 2 2H 2 O and Co(Ac) 2 4H 2 O solution, stirred at room temperature for 30 minutes; then transferred the mixture to an autoclave and continued to heat at 120°C for 2 hours, then cooled to room temperature; finally centrifuged to collect the precipitate, washed with absolute ethanol and pure water The precipitate was cross-washed 4 times, and the washed precipitate was dried at 120°C to obtain cobalt-doped zinc oxide nanoparticles;

[0036] (2) Cut the ITO conductive glass into a size of 1cm×4cm, and clean it with a mixed solution of ammonia water, hydrogen peroxide and water with a volume ratio of 1:1:50, then rinse it with water and dry it in the air for preparation and use .

[0037] (3) 20μL 1mg·ml -1 The cobalt-dope...

Embodiment 2

[0051] Embodiment 2: a kind of construction method for the photoelectrochemical sensor that acetamiprid detects, comprises the steps:

[0052] (1) First mix 50ml 1mol·L -1 NaOH solution was added dropwise to 100ml of Co 2+ Zn(Ac) with a molar ratio of 0.5% 2 2H 2 O and Co(Ac) 2 4H 2 O solution, stirred at room temperature for 40 minutes; then transferred the mixture to an autoclave and continued to heat at 110°C for 2 hours, then cooled to room temperature; finally centrifuged to collect the precipitate, washed with absolute ethanol and pure water The precipitate was cross-washed 3 times, and the washed precipitate was prepared at 110°C to obtain cobalt-doped zinc oxide nanoparticles;

[0053] (2) Cut the ITO conductive glass into a size of 1cm×4cm, and clean it with a mixed solution of ammonia water, hydrogen peroxide and water with a volume ratio of 2:2:50, then rinse it with water and dry it in the air for preparation and use .

[0054] (3) 20μL 1mg·ml -1 The cobalt...

Embodiment 3

[0062] Embodiment 3: a kind of construction method of the photoelectrochemical sensor that is used for acetamiprid detection comprises the following steps:

[0063] (1) First mix 50ml 1mol·L -1 NaOH solution was added dropwise to 100ml of Co 2+ Zn(Ac) with a molar ratio of 4% 2 2H 2 O and Co(Ac) 2 4H 2 O solution, magnetically stirred at room temperature for 60 minutes; then the mixture was transferred to an autoclave and continued to heat at 130°C for 2 hours, and cooled to room temperature; finally, the precipitate was collected by centrifugation, cross-linked with absolute ethanol and pure water washing the precipitate 5 times, and drying the washed precipitate at 130° C. to obtain cobalt-doped zinc oxide nanoparticles;

[0064] (2) Cut the ITO conductive glass into a size of 1cm×4cm, and clean it with a mixed solution of ammonia water, hydrogen peroxide and water with a volume ratio of 3:3:50, then rinse it with water and dry it in the air for preparation and use . ...

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Abstract

The invention discloses a method for constructing an acetamiprid detection photoelectrochemical sensor and a detection method, which comprises the steps of (1) regulating the molar ratio of Zn(Ac)22H2O to Co(Ac)24H2O in ethanol, and preparing Co-doped ZnO nanoparticles through the hydrothermal synthesis process; (2) cleaning and washing the ITO conductive glass, and airing the ITO conductive glass at the room temperature; (3) adding a Co-doped ZnO suspension liquid drop by drop onto the surface of an ITO electrode to prepare a Co-doped nano-ZnO film; (4) covering an acetamiprid aptamer onto the surface of the Co-doped nano-ZnO film to prepare an aptamer-based Co-doped ZnO photoelectrochemical sensor. According to the technical scheme of the invention, the acetamiprid and the aptamer are specifically bound together so as to cause the reduction of the photoelectric current. Based on the above principle, the quantitative detection is conducted. The invention has the advantages of simple equipment, simple and convenient method, good stability, high sensitivity and good selectivity.

Description

technical field [0001] The invention relates to a construction method and a detection method of a photoelectrochemical sensor for acetamiprid detection, belonging to the field of chemical analysis and testing. Background technique [0002] At present, the problem of pesticide residues has aroused widespread concern, because it has endangered human health and caused environmental pollution. As a neonicotinoid insecticide, acetamiprid has become a substitute for organophosphorus pesticides or other common pesticides, but its widespread use is still one of the potential hazards to human health. The current detection methods for acetamiprid include: gas, liquid chromatography, colorimetric aptasensor, enzyme-linked immunosorbent assay and electrochemical method. However, each method has one or more disadvantages as follows: expensive equipment, complicated method, poor stability, time-consuming, low sensitivity, general selectivity, etc. Therefore, seeking a convenient, simple...

Claims

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Application Information

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IPC IPC(8): G01N21/75
Inventor 方海林李红波李静王伟
Owner YANCHENG INST OF TECH
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