DNA chip and detection method and production method thereof
A technology of DNA chip and manufacturing method, which is applied in the field of DNA chip and can solve problems such as the absence of gene chip
Active Publication Date: 2015-11-04
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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However, there is no gene chip designed using SERS technology.
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[0023] In order to make the objectives, technical solutions and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with implementation examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0024] Firstly, it is stated that there are three types of DNA in the present invention: probe DNA, target DNA, and reporter DNA. The probe DNA is used to capture the target DNA, and the reporter DNA is used to send out detection signals.
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The present invention discloses a DNA chip and a detection method and a production method thereof. The substrate of the DNA chip is a SERS substrate taking a noble metal nano array as a substrate; the noble metal nano array is divided into a plurality of areas, the surface of each area is modified and connected with a plurality of different DNA probes, so that the DNA chip contains different probe DNA, the probe DNA is used to detect a target DNA to produce a SERS signal, and based on the frequency change and magnitude difference of the SERS signal, the identification and quantitative detection of the target DNA can be achieved. The DNA chip is designed by use of the noble metal nano array and surface enhanced raman scattering for achievement of detection and analysis of the target DNA and genes. The present invention also discloses a detection method and a production method of the DNA chip.
Description
technical field [0001] The invention relates to the fields of biological detection, biotechnology and nanotechnology, in particular to a DNA chip, a detection method of the DNA chip, and a manufacturing method of the DNA chip. Background technique [0002] DNA chip, also known as gene chip (genechip), is a kind of biochip, which refers to immobilizing a large number of probe molecules on a support, and hybridizing with labeled sample molecules according to the principle of complementary base pairing, and detecting the strength of the hybridization signal and distribution to obtain the sequence and quantity information of the target molecule. Supports for DNA chips include glass slides, silicon wafers, NC membranes, Nylon membranes, etc. DNA chip technology can be widely used in many fields such as disease diagnosis and treatment, optimal breeding of crops, judicial identification, food hygiene supervision, and environmental testing. The current DNA chip detection technolog...
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IPC IPC(8): C40B40/06C40B50/06C12Q1/68G01N21/65
Inventor 黄青孙克喜孟国文
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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