Check patentability & draft patents in minutes with Patsnap Eureka AI!

System for on-site environment monitoring

An environment, monitoring environment technology, applied in data processing applications, ICT adaptation, healthcare resources or facilities, etc., can solve problems such as high false positive rate

Inactive Publication Date: 2015-11-04
六品科技公司
View PDF7 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Additionally, there is a need for a system capable of distinguishing living from dead cells, since amplification methods based on modern molecules (such as nucleic acids) often do not distinguish between living or dead cells, which creates relatively high false positive rate

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • System for on-site environment monitoring
  • System for on-site environment monitoring
  • System for on-site environment monitoring

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0182] Example 1: A system for monitoring the environment

[0183] Identify thirteen testing sites on the floor plan of the food production facility. Test points include historical hotspots. The marked floor plan is converted to a png file and imported into software that identifies and tracks test locations within the floor plan. The marked test points are: quick freezing room (drainage system 1), quick freezing room (drainage system 2), quick freezing room (drainage system 3), quick freezing room (drainage system 4), cold storage (drainage system 5), quick freezing room (drainage system System 6), quick-freezing room (drainage system 7), quick-freezing room (balance), cold storage (guide flushing drainage system), quick-freezing room (oil drip tray 1), quick-freezing room (water receiver 2), quick-freezing room (forklift) and quick freezer (drainage close to the door). The test sites were divided into three groups for weekly testing.

[0184] Historical test data for this...

example 2

[0451] Example 2: Detection of bacteria with engineered phages

[0452] In this example, a relatively low number of E. coli NEB-10β cells was associated with 3xl0 8 PFU / mL of the engineered antibiotic T3::0.7 luc was mixed and luciferase production was measured every ten minutes by removing 20 μL of the lysate and combining it with 100 μL of the luciferin-containing detection reagent. ( Figure 11 . ) to prepare three different concentrations of cells in triplicate. Using 10-fold serial dilutions (from 10 0 to 10 -8 10-fold dilution of ) the number of bacteria present in each sample was determined after the experiment. Using this method, test samples were determined to have concentrations of E. coli of 6 (±4.6), 60 (±45.8) and 600± (458.0) cells. For the two higher concentrations (60 and 600), maximum light production in the sample was observed 40 min after the addition of the phage. However, a detectable signal was measured above the lower limit of detection at 20 minu...

example 3

[0455] Example 3: Detection of bacteria with engineered phages

[0456] The experiment was designed and performed to determine the minimum time of detection in the T3::0.7 luc / E. coli pairing.

[0457] Logarithmic phase culture of E. coli (2.5xl0 7 CFU / mL) by 3xl0 8 Infect with PFU / mL of T3::0.7 luc and aliquot 20 μL in microtubes. Every ten minutes, triple reads were obtained by measuring light production from triplicate aliquots by adding 100 μL of Promega luciferase assay reagent. exist Figure 12 The results are shown in .

[0458] We found that the first time we measured cultures at 10 min post-infection, there was already a substantial signal for detection (51,393 ± 28,152 RLU). As in previous experiments, the time to maximize the generated signal was 40 minutes.

[0459] Extrapolated backward from the 10 minute time point, these data imply detection at 5 minutes.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Methods and systems are provided herein for monitoring pathogens in various environments and on various items, wherein data from monitoring is trackable, analyzable and comparable versus various standards or thresholds. The methods and systems disclosed herein also include a platform for managing the detection and reporting of pathogens across a number of locations within a number of environments, and using such detection for a wide variety of purposes.

Description

Background technique [0001] Results from various environmental sampling and detection methods are typically presented to the user in a manner that is not always real-time or actionable. Additionally, results are not always delivered in a manner that makes them trackable, analyzable, or comparable against various standards or thresholds. Therefore, there is a need for an on-line system for real-time or near real-time monitoring and analysis. Such a system can be used in conjunction with a rapid, reliable in-situ sampling method to provide an on-site environmental monitoring platform. [0002] Methods and systems exist for the detection of bacteria such as Listeria spp. that pose a health hazard in food, food preparation / food service environments, and other environments including hospitals, universities, any manufacturing facility where environmental microorganisms are controlled, and related facilities. ), Salmonella (Salmonella) and Escherichia coli ( E. coli ) such microor...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G06F17/18G06Q10/06
CPCG06Q10/06G06F19/327G16H40/20Y02A90/10
Inventor M.J.罗森布卢姆E.塔基安M.S.克里斯T.K.卢
Owner 六品科技公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com