Culture method of tumor specific TIL cells

A culture method and cell technology, applied in the biological field, can solve the problems of allergies, spread of infectious diseases, complex preparation technology of TIL cells, etc., and achieve the effect of improving tumoricidal effect and simple operation.

Inactive Publication Date: 2015-11-25
SHANGHAI XINCHEN MEDICAL TECH CO LTD
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] However, the preparation technology of TIL cells is complicated, and it is usually necessary to screen hundreds or even hundreds of T lymphocyte clones to obtain tumor-specific TIL cells. The disadvantage of animal serum is the risk of potential transmission of infectious diseases, and the occurrence of allergies due to foreign proteins, while the supply of human AB serum is limited, and there is also a risk of transmission of infectious diseases
These factors severely limit its application and effectiveness

Method used

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  • Culture method of tumor specific TIL cells
  • Culture method of tumor specific TIL cells

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Embodiment Construction

[0024] Unless otherwise indicated or defined, all terms used have their ordinary meanings in the art, which will be understood by those skilled in the art.

[0025] Referring to, for example, standard manuals, the practitioner is referred to standard texts and commentaries on cell biology, histology and embryology for further details on conventional techniques employed in the practice of the present invention. 包括Teratocarcinomasandembryonicstemcell:Apracticalapproach[E.J.Robertson编,IRL出版有限公司,1987];GuidetotechniquesinMouseDevelopment[P.M.Wasserman等编,学术出版社,1993];EmbryonicStemCellDifferentiationinVitro[M.V.Wiles,Meth.Enzymol.225:900,1993];PropertiesandusesofEmbryonicStemCells:ProspectsforApplicationtoHumanBiologyandGeneTherapy [P.D. Rathjen et al., Reprod. Fertil. Dev. 10:31, 1998].

[0026] Cell biology, protein chemistry, and antibody technology can be found in "Current Protocols in Protein Science" [J.E. Colligan et al., Wiley & Sons], "Current Protocols in Cell Biology" [J.S. ...

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Abstract

The invention belongs to the field of biomedical detection, and in particular relates to a culture method of tumor specific TIL cells. According to the culture method of the tumor specific TIL cells disclosed by the invention, the tumor specific TIL cells can be acquired by screening specific magnetic beads twice. The method disclosed by the invention is simple in operation, and is capable of efficiently obtaining the tumor specific TIL cells through multiplication culture and greatly enhancing the tumor-cytotoxicity effect of the TIL cells; and meanwhile, through serum-free culture, the method can solve the potential risks of transmission of viruses and other dangers in the prior art, so as to provide a foundation for extensive application in the future.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for culturing tumor-specific TIL cells. Background technique [0002] Adoptive immunotherapy (ACI) refers to infusing immune cells with anti-tumor activity into tumor patients to directly kill or stimulate the body's immune response to kill tumor cells, so as to achieve the purpose of treating tumors. It includes non-specifically activated effector cells and specifically activated effector cells. The former uses non-specific stimulatory factors (IL2, interferon) to stimulate precursor effector cells to activate them into effector cells with anti-tumor activity, such as LAK cells, tumor infiltration Cytokine-induced kill cells (CIK), etc.; specifically activated effector cells refer to anti-tumor effector cells induced by using tumor antigens as stimulants, such as dendritic cells (DC) , cytotoxic T lymphocytes (CD8+ cells), etc. The application of ACI in the tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0783
Inventor 钱兆与
Owner SHANGHAI XINCHEN MEDICAL TECH CO LTD
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