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AaTSW1 gene promoter and application and preparation method thereof

A promoter and gene technology, applied in the field of genetic engineering, can solve problems such as harm, normal growth burden of plants, etc.

Active Publication Date: 2015-12-02
上海阿提密斯生物科技发展有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This promoter can drive the expression of foreign genes in all tissues and organs, so it is likely to cause a certain burden and harm to the normal growth of plants

Method used

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  • AaTSW1 gene promoter and application and preparation method thereof
  • AaTSW1 gene promoter and application and preparation method thereof
  • AaTSW1 gene promoter and application and preparation method thereof

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Embodiment Construction

[0031] The specific embodiments provided by the present invention will be described in detail below in conjunction with the accompanying drawings.

[0032] The experimental method that does not indicate specific conditions in the following examples usually follows conventional conditions, such as molecular cloning such as Sambrook: the laboratory manual sees the conditions described in the 1989 edition of NewYork:Cold Spring Harbor Laboratory Press, or according to the conditions suggested by the manufacturer.

[0033] The Agrobacterium tumefaciens EHA105 involved in this embodiment has been described in "Huang Yali, Jiang Xiliang, Tian Yunlong, Guo Ping, Zhu Changxiong; Research on the genetic transformation of Trichoderma harzianum mediated by Agrobacterium tumefaciens, Chinese Journal of Biotechnology, 2008, 28( 3): 38-43" published in the literature. The plasmid pCAMBIA1391Z of Agrobacterium tumefaciens EHA105 can be obtained through public commercial channels, for example...

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Abstract

The invention discloses an AaTSW1 gene promoter and a preparation method thereof. A nucleotide sequence of the promoter is shown as a first sequence in a sequence table. Meanwhile, the invention further relates to application of the AaTSW1 gene promoter in the genetic engineering breeding in which metabolic products are expressed and produced by means of glandular hair tissue of a plant. According to the AaTSW1 gene promoter and the application and preparation method thereof, the promoter can guide genes to be specifically expressed in a glandular hair of the plant, and due to the fact that the promoter which is specifically expressed in the glandular hair performs genetic manipulation on a glandular hair system of the plant, harm cannot be caused to the growth and development of the plant; accordingly, the important significance is achieved for genetic engineering breeding in which the metabolic products are expressed and produced by means of the glandular hair tissue of the plant.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a gene promoter and its application and preparation method. Background technique [0002] Artemisia annua L. is an annual herb belonging to the family Asteraceae. Artemisinin is a sesquiterpene lactone compound containing a peroxide bridge structure isolated from its aerial part. It is currently recognized as the most effective drug for treating malaria in the world, especially for cerebral malaria and chloroquine-resistant malaria. efficient. Currently, the most effective treatment for malaria recommended by the World Health Organization is artemisinin combination therapy (ACTs). At present, the main source of artemisinin is extracted from the aerial parts of Artemisia annua plants. However, the content of artemisinin in Artemisia annua is very low (0.01%-1%), which limits the large-scale commercial production of this drug. [0003] AaTSW1 is a WRKY transcription ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/84C12N1/21C12N15/10A01H5/00C12R1/01
Inventor 唐克轩陈明慧颜廷祥付雪晴石璞马亚男郝小龙孙小芬
Owner 上海阿提密斯生物科技发展有限公司
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