21 results about "Glandularia" patented technology

Provided is a PagHB7a gene related to the salt tolerance and drought tolerance of poplar. The gene is used to create new germplasms of salt-tolerant and drought-tolerant 84K poplar (Populus alba×Populus glandulosa). Being a candidate gene related to the salt tolerance and drought resistance of poplar, the PagHB7a gene provides an important gene resource for breeding new varieties of salt-tolerant and drought-resistant poplar, and can be directly used for molecular-assisted breeding of stress-resistant poplar. In addition, a primer pair for amplifying the candidate gene and a transgenic modification method for poplar are provided, which can evaluate the stress resistance of poplar on the molecular level; on this basis, varieties with remarkable stress resistance can be accurately and efficiently screened, thus effectively shortening the breeding period of poplar.

The application provides a method for observing glandular hairs of Ainsliaea sinensis by using a scanning electron microscope. The method comprises the following steps: (1) selecting a region to be measured in a leaf blade, cutting a sample, and immersing the sample in an aldehyde fixing solution for fixation and immersion; (2) performing gradient dehydration by using an ethanol solution, performing replacement by using isopentyl acetate, and then performing drying by using a critical point drying instrument; (3) removing non-glandular hairs by using adhesive tape; and (4) then spraying gold and observing under a scanning electron microscope. By using the method, the observation and distribution density statistics of the glandular hairs of Ainsliaea sinensis can be simply, efficiently and accurately completed. By using the method, the problem of uneven leaf blade is well solved, and the problem of non-glandular hairs shielding and interfering with the visual field is completely solved.

The invention relates to an NtWUN gene as well as an expression product, an expression vector and application of the NtWUN gene in regulation and control of plant glandular hair development, and aims to solve the technical problems that the development mechanism of different types of epidermal hairs in plants (such as tobacco) is unknown and effective molecular regulation and control measures and utilization ways are lacked at present. According to the invention, an NtWUN gene for negatively regulating epidermal hair development is identified, a gene editing vector and an overexpression vector are constructed, and a gene editing homozygous strain and an overexpression homozygous strain are obtained through screening; tissue expression, MeJA response mode analysis, subcellular localization, plant phenotypic characteristic identification and the like show that the NtWUN can negatively regulate the occurrence of epidermal hair and influence the change of the total density of the epidermal hair, and the NtWUN has important application value in regulating the development process of the plant epidermal hair.

The present invention discloses a method and apparatus for electrostatic separation of trichomes from plant biomass, addressing the limitations of conventional separation techniques. The method relies on differences in electrical properties of trichomes and plant material, utilizing an external electric field. A multi-component electrostatic separation assembly may prepare and separate one or more samples, thereby incorporating a dispensing component, a pipeline component, and a separation chamber comprising at least one electrode assembly. Moreover, the present invention may also provide for electrode assemblies that feature self-cleaning mechanisms.

The present invention relates to polynucleotide comprising a lncRNA gene according to SEQ ID No: 2, or according to a sequence having at least 50% sequence identity to SEQ ID No: 2, and an AP2c gene according to SEQ ID No: 3, or according to a sequence having at least 50% sequence identity to SEQ ID No: 3, which polynucleotide confers an increased density of type IV trichomes. The invention further relates to a plant comprising said polynucleotide and a SlASAT4 gene according to SEQ ID No. 21, or comprising a homologous sequence having at least 99.6% sequence identity to SEQ ID No. 21 wherein the presence of the polynucleotide leads to increased density of type IV trichomes and wherein the presence of said SlASAT4 gene leads to an increased ratio of S4:17 / S3:15 acyl sugars produced in glandular trichomes present on said plant when compared to a control plant lacking the polynucleotide and the SlASAT4 gene. Such a plant also was found to be resistant to Bemisia tabaci. The invention also discloses markers for the identification of the polynucleotide and the SlASAT4 gene and their use for the identification and development of Solanum lycopersicum plants. Also methods for identifying, producing and selecting of plants and seed are disclosed.

Glandular trichomes are epidermal outgrowths in plants that are the site of metabolic compound synthesis and storage. Modulating the secretion of metabolite compounds in tobacco and tea leaves can alter the taste profile in products made from these plants. The present disclosure relates to methods for modulating secretion from glandular trichomes and compositions comprising altered trichome secretion of metabolites.

Glandular trichomes are epidermal outgrowths in plants that are the site of metabolic compound synthesis and storage. Modulating the secretion of metabolite compounds in tobacco and tea leaves can alter the taste profile in products made from these plants. The present disclosure relates to methods for modulating secretion from glandular trichomes and compositions comprising altered trichome secretion of metabolites.

The present invention relates to a method, device and storage medium for identifying the leaf-fuzz ratio grade of moxibustion materials, which are applied to the field of traditional Chinese medicine technology, and include: obtaining the cellulose content of moxa floss and the length of non-glandular hairs of moxa floss. Through pre-experiment calculation, it can be known that the length and cellulose content of moxa floss have the highest contribution rate to various combustion indexes. That is to say, the heat release performance of moxa floss combustion is closely related to the length of non-glandular hairs and the cellulose content of moxa floss. And according to the moxa floss with known leaf-fuzz ratio grades, the length of non-glandular hairs and the cellulose content of different leaf-fuzz ratios can be known, that is, the standard value range of the length of preset non-glandular hairs and the standard value range of cellulose in this application. According to which leaf-fuzz ratio grade's standard value range the length of non-glandular hairs and the cellulose content of the moxa floss with unknown leaf-fuzz ratio are in, the leaf-fuzz ratio of the moxa floss can be obtained, that is, the quality of the moxa floss with unknown leaf-fuzz ratio is identified.

The invention discloses pseudomonas fluorescens SMP5 which is derived from rhizosphere soil of salvia miltiorrhiza. The invention further provides application of the pseudomonas fluorescens SMP5 in growth promotion and quality improvement of the artemisia annua, the pseudomonas fluorescens SMP5 obtained through screening and identification can achieve efficient colonization on the surfaces of leaves of the artemisia annua, and plant growth and quality improvement are remarkably promoted in a foliage spraying mode. Compared with an existing traditional method depending on chemical fertilizer or exogenous hormone, the strain can effectively promote leaf yield and biomass accumulation (the yield and the biomass are increased by 41.04% and 44.92% respectively), the secretory glandular hair density is remarkably increased by 4.4 times, and more structural bases are provided for artemisinin synthesis. According to the method, a complete regulation and control chain of beneficial bacterium-leaf surface-glandular hair-artemisinin synthesis is established, double improvement of quality and yield is achieved, and the method has the advantages of being environmentally friendly, safe and capable of being popularized.

The invention discloses an application of plant epidermal hair instability in increasing the iron content of plant leaves. Belongs to the technical field of plant nutrient enrichment. The invention provides a method for increasing the iron content in a plant leaf, and the iron content in the plant is increased by destabilizing epidermal hair. The method is suitable for plants with non-glandular hair distributed on leaves such as arabidopsis thaliana and Chinese cabbage, hunger response of iron ions in the leaves is caused through methods such as leaf epidermal hair touch treatment, then the iron content in the plant leaves is increased, and the method has the advantages of being short in time, simple and easy to operate.

The application relates to the technical field of plant molecular biology, and particularly discloses a tomato gland-type epidermal hair regulating gene SlWRKY42 and application thereof, wherein the ORF sequence of the gene SlWRKY42 is shown as SEQ ID NO. 1. By regulating the overexpression of the SlWRKY42 gene in a tomato plant, the density of tomato gland hairs is significantly increased, the transcription and expression of an eneyterpene synthase gene (SlTPS12) and a precursor synthesis pathway transgene are significantly up-regulated, and the content of eneyterpene substances is significantly increased. The gene SlWRKY42 provided by the application can regulate the growth and development of tomato gland-type epidermal hairs, and simultaneously regulate the synthesis of eneyterpene substances.

The present invention relates to a method of producing a plant with an increased density of glandular trichomes and / or with an increased production of metabolite(s) comprising a step of introducing at least one genetic alteration that increases activity of PRODERMAL FACTOR2 (PDF2) of said plant, wherein PDF2 preferably has an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 60, SEQ ID NO: 62, SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89 and SEQ ID NO: 90.

The invention discloses a mint McHD-Zip1 gene promoter and application thereof, and relates to the technical field of gene engineering. According to the mint McHD-Zip1 gene promoter disclosed by the invention, the nucleotide sequence of the mint McHD-Zip1 gene promoter is as shown in SEQ ID NO. 1. When the promoter is used for constructing a plant expression vector pGC-GUS-McHD-Zip1pro, the result of the embodiment shows that the promoter can drive a target gene to be predominantly expressed in stem tips, leaf axils and secretory glandular hairs of a plant instead of being widely expressed in various tissues and organs of the whole plant, so that the adverse effect on normal growth and development of the plant can be reduced, and the plant expression vector pGC-GUS-McHD-Zip1pro can be used for preparing the plant expression vector pGC-GUS-McHD-Zip1pro. The metabolism or development process of the specific part of the plant can be more accurately regulated and controlled; compared with a constitutive promoter, the specific promoter avoids interference of whole plant expression on plant metabolic balance, and provides a precise tool for studying plant glandular hair development and metabolic engineering.

An electrostatic separation apparatus and method are provided for isolating glandular trichomes from trichome-bearing plant biomass such as cannabis, hemp, or hops. The system may include a vertically oriented particle transport assembly configured to convey a sample through a linear free-fall pathway, a separation chamber with opposing electrode assemblies generating a uniform electrostatic field, and discharge electrodes positioned below the chamber to neutralize residual charge. Airflow regulation may be achieved through laminar diffuser plates and periodic vibration of critical components, including the hopper and diffuser, to prevent clogging and maintain uniform flow. The hopper may further include a circular discharge spout and rounded internal fillets to prevent bridging and ensure consistent powder recirculation. The apparatus achieves high-purity trichome separation through controlled field exposure, aerodynamic stabilization, and charge-neutralized particle discharge, enabling continuous, high-throughput operation with improved yield, reduced maintenance, and enhanced reproducibility across successive processing cycles.

The present invention discloses a plant glandular hair head gene GHF1 The present invention identifies a gene that can negatively regulate the development of plant glandular hair heads. GHF1 , constructed its overexpression vector and gene editing vector, and carried out tobacco transformation respectively to obtain the corresponding overexpression strains and gene knockout plants; the epidermal hair morphology identification and leaf chemical composition analysis showed that GHF1 The gene can negatively regulate the occurrence and development of the glandular hair head of plants, and has important application value in the regulation of glandular hair development and the synthesis of glandular hair-specific substances.

The invention discloses an application of a cucumber CsLSGT gene as a target spot in regulating formation of a silicification layer on the surface of a fruit gland hair. The formation of the silicification layer on the surface of the cucumber gland hair is reduced by reducing the expression quantity of the CsLSGT gene. It is found through screening that the cucumber CsLSGT gene is related to formation of a silicification layer on the cucumber fruit gland hair surface. In a cucumber mutant, the silicon element content of fruit glandular hair is obviously reduced compared with that of a wild type '649'. Meanwhile, a grafting experiment shows that the function of the CsLSGT gene depends on the overground part, which indicates that the reduction of the silicon element on the surface of the glandular hair is mediated by an overground part factor. The gene can be used for screening cucumber fruits with few silicon elements and improving the commodity quality traits of the cucumber fruits.

This invention discloses a gene regulating plant glandular trichome development. NtJAZ11, This application aims to address the current technical problem of unclear developmental mechanisms and lack of effective regulatory methods and utilization pathways for glandular trichomes and protective trichomes in plants (such as tobacco). The gene encoding this gene, its expression vector, and its applications are described. NtJAZ11 Overexpression and gene-editing vectors were constructed, and corresponding overexpression and gene-editing lines were obtained through transformation. Subcellular localization, tissue expression, MeJA response pattern analysis, and plant phenotypic identification showed that... NtJAZ11‑2 It can negatively regulate the development of tobacco glandular hairs, and has important application value in regulating the development of plant epidermal hairs.

This invention application discloses a gene regulating plant glandular trichomes. NtMIXTA This application relates to genes, their encoded proteins, biological materials, and applications, aiming to address the technical problem of low efficiency in increasing plant glandular trichome density using traditional breeding techniques. This application clones a gene from tobacco... NtMIXTA Genes, and constructed separately NtMIXTA Gene knockout and overexpression lines were found NtMIXTA Gene knockout reduced leaf glandular hair density, while overexpression lines showed a significant increase in leaf glandular hair density, indicating that... NtMIXTA Genes positively regulate the occurrence and development of glandular trichomes in tobacco. This application is of great significance for studying the molecular regulatory network of plant glandular trichome formation.

The invention discloses application of an NtHCH5 gene in regulation and control of plant glandular hair development, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the gene for regulating and controlling plant glandular hair development provided by the invention is as shown in SEQ ID NO. 1. An RNAi carrier of the NtHCH5 gene is constructed through an RNAi technology, a tobacco plant silenced by the NtHCH5 gene is cultivated, and it is found that compared with a normal tobacco plant, the density of glandular hairs in tobacco leaves after the NtHCH5 gene is silenced is remarkably reduced, and the glandular hairs are shortened. An economic plant tobacco is used as a research object, and experiments prove that the NtHCH5 gene participates in regulation and control of plant glandular hair development, a new target gene is provided for regulation and control of plant glandular hair development, and a gene regulation and control network of plant in-vivo epidermal hair is enriched. The gene function of the plant NtHCH5 gene is also enriched, and the gene has important significance for analyzing the diversity of the plant NtHCH5 gene.

The invention particularly discloses a method for inducing transdifferentiation of paving cells in mature leaves of nicotiana benthamiana into glandular hair cells, which comprises the following steps: preparing an agrobacterium infection solution, and agrobacterium is selected from one or more of a GV3101 strain, a C58C1 strain or a K599 strain; carrying out infection treatment on completely expanded mature leaves of the nicotiana benthamiana by using an agrobacterium infection solution; and after infection, the nicotiana benthamiana is cultured, and the paving cells of the leaves are induced to be transdifferentiated into glandular hair cells. The method is easy to operate and good in repeatability, stable genetic transformation and strict sterile conditions are not needed, the paving cells of the Bensi tobacco leaves can be induced to be transdifferentiated into the glandular hair cells, and increase of the number of the glandular hair cells is promoted.

The invention discloses a mint transcription factor gene McHD-Zip1 as well as an expression protein and application thereof, and relates to the technical field of gene engineering. The full-length coding sequence of the mint transcription factor gene McHD-Zip1 disclosed by the invention is as shown in SEQ ID NO.1, and the amino acid sequence of the expression protein of the mint transcription factor gene McHD-Zip1 is as shown in SEQ ID NO.2. An overexpression vector of the mint transcription factor gene McHD-Zip1 is constructed and transformed into peppermint, and the result of the embodiment shows that the McHD-Zip1 can regulate and control the development of glandular hair of mint leaves, and overexpression of the gene can remarkably increase the glandular hair density of the leaves and regulate and control the content of total volatile oil, menthone and limonene in mint bodies. The invention provides a new gene resource for research on regulation and control of plant glandular hair development, and also provides theoretical support and gene resources for improvement of mint germplasm and improvement of the yield and quality of mint essential oil.