38 results about "Glandularia" patented technology

The invention relates to the technical field of gene engineering, in particular to an application of GRAS11 to regulation of synthesis of plant terpenoids and / or development of glandular hairs. According to the invention, firstly, amplification is performed in Solanumlycopersicum L. to obtain a transcription factor GRAS11, and the transcription factor GRAS11 is specifically expressed in glandular hairs of leaves and stems. The overexpression of GRAS11 can significantly enhance the expression level of terpene synthase and upstream precursor synthetic pathway genes in tissues such as leaves, thereby improving the yield of terpenoids and increasing the volume of glandular hairs. The invention provides beneficial values for research and application in aspects of improving crop nutritional quality, improving crop insect resistance and disease resistance and the like in genetic engineering.

The invention relates to an artemisia apiacea translocator AaPDR3 and an application thereof. The amino acid sequence is shown as SEQ ID No.2; the artemisia apiacea translocator AaPDR3 can affect the synthesizing of sesquiterpenes in artemisia apiacea non-secreting type glandular hair; the translocator is encoded by the nucleotide sequence shown as SEQ ID No.1. The invention further provides a method for realizing transgenosis artemisia apiacea seedlings with the artemisia apiacea translocator AaPDR3.

The invention discloses a method for increasing the aroma content of tobacco leaves and the tobacco leaves with high aroma content, belongs to the technical field of agriculture, and solves the problem that a method for effectively improving aroma content of tobacco leaves by spraying a glandular hair extract and a monopotassium phosphate solution with a certain proportion concentration before tobacco leaves are baked and the problem that the tobacco leaves with high aroma content are obtained after the tobacco leaves are baked by adopting the method are lacked in the prior art. The method mainly comprises the steps: applying fermented soybeans during tobacco transplanting; before tobacco leaves are baked, spraying a glandular hair extract and a monopotassium phosphate solution. The methodis mainly used for increasing the content of aroma substances in the tobacco leaves.

The invention discloses an AaTSW1 gene promoter and a preparation method thereof. A nucleotide sequence of the promoter is shown as a first sequence in a sequence table. Meanwhile, the invention further relates to application of the AaTSW1 gene promoter in the genetic engineering breeding in which metabolic products are expressed and produced by means of glandular hair tissue of a plant. According to the AaTSW1 gene promoter and the application and preparation method thereof, the promoter can guide genes to be specifically expressed in a glandular hair of the plant, and due to the fact that the promoter which is specifically expressed in the glandular hair performs genetic manipulation on a glandular hair system of the plant, harm cannot be caused to the growth and development of the plant; accordingly, the important significance is achieved for genetic engineering breeding in which the metabolic products are expressed and produced by means of the glandular hair tissue of the plant.

The invention belongs to the field of plant gene engineering and discloses a reproductive organ and glandular hair tissue specific promoter GhS and the application of the promoter. The promoter is specifically express in arabidopsis thaliana anthers and stigmatoid tissues, petals, sepal glandular hair, leaf glandular hair, seed funiculi and epidermal hair of part of seeds. A nucleotide sequence ofthe promoter GhS is shown as SEQ ID NO. (sequence identifier number) 1. The promoter GhS has the following characteristics: A, an expression situation in an arabidopsis thaliana tissue is very clear;the expression of the promoter on plants such as cotton is not reported; B, the sequence of the promoter is from a cotton ovule; the specific expression of the promoter in the arabidopsis thaliana glandular hair implies that the promoter can specifically regulate the expression of a target gene in cotton fibers; and C, the promoter is novel in structure and has no homology with other promoters reported on a nucleic acid level; and homology sites do not contain the existing patent protected sequences and mutant sites.

The invention discloses application of a clematis terniflora isopentenyl transferase PT1 gene, an overexpressed arabidopsis strain thereof and a construction method of the overexpressed arabidopsis strain. The nucleotide sequence of the clematis terniflora isopentenyl transferase PT1 gene is as shown in SEQ ID NO. 1. A model plant arabidopsis thaliana is adopted, and a positive strain of arabidopsis thaliana trans-PT1 gene is obtained by constructing a plant PT1 gene overexpression vector and transgenosis operation. The invention proves that the plant morphology of the transgenic arabidopsis thaliana strain has the characteristics of multiple glandular hair on the leaf surface and dark leaf color. After ultraviolet stress, the O2 <-> generation rate and H2O2 content increase in the transgenic arabidopsis thaliana leaves are remarkably lower than those of wild arabidopsis thaliana, the MDA content increase in the leaves is remarkably lower than those of the wild arabidopsis thaliana leaves, and the leaf conductivity increase is remarkably lower than that of the wild arabidopsis thaliana leaves. After extreme ultraviolet stress, the survival rate of the transgenic arabidopsis thaliana is remarkably higher than that of wild arabidopsis thaliana.

The invention relates to an AaPDR2 gene promoter as well as a functional verification method and application thereof. The AaPDR2 gene promoter comprises the following cis-acting elements: ABRE, BoxI, CAAT-box, CCAAT-box, CGTCA-motif, G-Box, G-box, GA-motif and TATA-box. Further, the DNA (Deoxyribonucleic Acid) sequence of the AaPDR2 gene promoter is shown in SEQ ID NO.1. The AaPDR2 gene promoter has a function of predominantly expressing genes guided by the AaPDR2 gene promoter in glandular secretory trichome and T shape trichome of young leaves of Artemisia annua L., and the function is confirmed by a GUS reporter gene. Therefore, the AaPDR2 gene promoter disclosed by the invention has important significance for genetic engineering breeding for expressing and generating metabolites by using plant glandular trichome tissues.

The present invention relates to a modified Slmyc2 gene, which may comprise at least one modification as compared to the wild type sequence of SEQ ID No. 5, which modification leads to the reduction or absence of SlMYC2 protein activity, wherein the modified Slmyc2 gene is capable of conferring an aberrant glandular hair phenotype to a Solanum lycopersicum plant. The modification may be suitably selected from a modification that decreases the mRNA level of the Slmyc2 gene, a modification that decreases the level of the SlMYC2 protein and / or a modification that decreases the activity of the SlMYC2 protein, as compared to the wild type Slmyc2 gene of SEQ ID No. 5.

The invention discloses a method for identifying lines of lonicera japonica thunb (Damaohua and Jasminum duclouxii) based on restrictive fragment length polymorphism polymerase chain reaction (PCR-FRLP). The invention provides a kit for identifying lines of lonicera japonica thumb (Damaohua and Jasminum duclouxii) which comprises a primer pair composed of two single-stranded DNA molecules as shown in sequence 1 and sequence 2 in a sequence table as well as restriction enzyme MfeI or isoschizomer thereof. The method disclosed by the invention, by analyzing COi gene sequences related to development of glandular hairs of lines of lonicera japonica thumb (Damaohua and Jasminum duclouxii), can obtain a difference SNP site of Damaohua and Jasminum duclouxii; identification primers and corresponding restriction enzyme are designed; and accurate identification of two lines of the lonicera japonica thumb is realized through the PCR-FRLP technology.

The invention belongs to the field of plant genetic engineering, and discloses a reproductive organ and glandular trichome-specific promoter GhS and its application. The trichomes of stalks and some seeds are specifically expressed, and the nucleotide sequence of the promoter GhS is shown in SEQ ID NO.1. The GhS promoter of the present invention has the following characteristics: A, the expression situation in Arabidopsis tissue is very clear, and this promoter has not been reported on plants such as cotton; B, this promoter sequence comes from cotton ovule, and it is in Arabidopsis The specific expression in the mustard glandular hairs implies that it can specifically regulate the expression of the target gene in cotton fibers; C, the structure is new, there is no homology with other promoters that have been reported at the nucleic acid level, and the homology position The point does not contain the existing patent protection sequence and mutation site.

The invention relates to a microscopic slide preparation method for observing the characteristics of the lower epidermis of folium artemisiae argyi, which effectively solves the problem of observing the pore of the lower epidermis of folium artemisiae argyi, the vertical peripheral wall pattern of epidermis cells and the distribution of glandular hair and non-glandular hair by using an optical microscope. Rinsing with distilled water, soaking with a mixed solution of sodium hydroxide and hydrogen peroxide, dissociating mesophyll tissues, bleaching, transferring into alcohol, and removing non-glandular hairs on leaf epidermis; the preparation method comprises the following steps of: adding the raw materials into a mixed solution of chromic acid and nitric acid, standing, dissociating mesophyll tissues, rinsing by using alcohol, separating upper and lower epidermis, removing mesophyll tissues of inner epidermis, dyeing the upper and lower epidermis by using a toluidine blue O solution, and rinsing by using water to remove floating color and water seal sheets, thereby obtaining the high-purity mesophyll tissue. The preparation method is simple to operate, low in cost, small in workload, high in success rate, clear in observation and good in effect; the technical problem of observing the lower epidermis of the folium artemisiae argyi is solved, the technical requirement on operators is not high, and an ideal effect can be achieved.

The invention provides a gene promoter, belonging to the field of plant biotechnology. The gene promoter has a nucleotide sequence as shown in SEQ ID No. 1. The AaTCP15 gene promoter can regulate theexpression of a target gene in all the tissue of a plant, especially preferential expression in secretory glandular trichomes and non-secretory glandular trichomes. Meanwhile, the invention also relates to application of the AaTCP15 gene promoter to genetic engineering breeding which employs the glandular trichome tissue of plants for expression and production metabolites. Since genetic manipulation of the promoter preferentially expressed in glandular trichomes on the glandular trichome system of plants has no harm to the growth and development of the plants, the AaTCP15 gene promoter provided by the invention is of critical significance to genetic engineering breeding which employs the glandular trichome tissue of plants for expression and production metabolites.

The invention provides a transcription factor CsAPL1 and an application thereof and belongs to the technical field of plant gene engineering. According to the transcription factor CsAPL1 and the application thereof, the transcription factor CsAPL1 is separated from glandular hair of plants, i.e., cannabis for the first time and has a nucleotide sequence shown in SEQ ID NO: 1, and an encoded aminoacid sequence is shown in SEQ ID NO: 2. According to the transcription factor CsAPL1 and the application thereof, proven by functional tests, the transcription factor CsAPL1 can interact with a THCA synthetase (THCAS) gene promoter, so that the transcription factor CsAPL1 exerts an activation action in synthesis of cannabine compounds, and regulation and control actions on the synthesis of the cannabine compounds can be effectively achieved.

The invention discloses a tobacco glandular hair development regulator, a tobacco upper leaf growth promoting and secretion increasing leaf fertilizer and preparation and application of the tobacco upper leaf growth promoting and secretion increasing leaf fertilizer, and aims at solving the technical problem that in the prior art, growth and development of tobacco upper leaf glandular hair are difficult to effectively regulate and improve through cultivation technical measures. The glandular hair development regulator contains 400-600 [mu]M of methyl jasmonate and 80-120 [mu]M of gibberellin, and 1.5-2.5 mM of potassium sulfate and 0.4-0.6 mM of calcium nitrate are further added to prepare the growth promoting and secretion increasing leaf fertilizer. The components have a synergistic effect, a remarkable induction effect on growth and development of the upper tobacco leaf glandular hair is achieved, and after application, the glandular hair can become robust, the density of the glandular hair is increased, the head of the glandular hair becomes large, the content of glandular hair secretions is remarkably increased, and the plant resistance is improved. The advantages of few types of required raw materials, simple preparation, low cost, convenient application, and easy large-area popularization and application are achieved.

The present invention relates to a modified Slmyc2 gene, which may comprise at least one modification as compared to the wild type sequence of SEQ ID No. 5, which modification leads to the reduction or absence of SlMYC2 protein activity, wherein the modified Slmyc2 gene is capable of conferring an aberrant glandular hair phenotype to a Solanum lycopersicum plant. The modification may be suitably selected from a modification that decreases the mRNA level of the Slmyc2 gene, a modification that decreases the level of the SlMYC2 protein and / or a modification that decreases the activity of the SlMYC2 protein, as compared to the wild type Slmyc2 gene of SEQ ID No. 5.

The invention relates to a method for observing epidermal hair of a plant leaf by using a fluorescence microscope. The method can effectively solve the problems of clearly observing the type and distribution of the epidermal hair of the plant and ensuring the quality of medicinal materials by using the fluorescence microscope, and comprises the following steps of: cutting off mature leaves from aplant, cutting off 5-10 mm<2> leaves without thick veins on two sides of the middle section of the middle vein of each leaf, putting the cut leaves into methanol, removing chlorophyll by using ethanol, soaking in berberine, treating by using vanillin-concentrated hydrochloric acid, rinsing, sealing, observing the types and distribution of glandular hairs and non-glandular hairs by using an epifluorescence microscope, and shooting. The method is simple to operate, low in cost, small in workload, high in success rate, clear in observation and good in effect, can completely solve the problem of observation of various plants with leaf epidermis densely covered by non-glandular hairs and glandular hairs, can achieve an ideal effect by not having good requirements on an operation technology, isan innovation in plant structure observation, and has very strong practicability and remarkable economic and social benefits.

The invention relates to an AoORA gene promoter, wherein the nucleotide sequence of the promoter is shown in SEQ ID NO.1; and in addition, the invention further relates to application of the AoORA gene promoter in genetic engineering breeding of utilizing plant glandularia tissues to express and produce metabolite. The promoter provided by the invention is capable of inducing genes to be specifically expressed in plant glandularia without bringing damage to the growth and development of plants because the promoter specifically expressed in glandularia carries out genetic operation on the glandularia systems of the plants, thereby being significant in genetic engineering breeding of utilizing the plant glandularia tissues to express and produce metabolite.

The invention, which belongs to the technical field of plant genetic engineering, provides a transcription factor CsMYB1 and application thereof. A transcription factor CsMYB1 is separated from plantcannabis sativa glandular hairs for the first time, the nucleotide sequence of the transcription factor CsMYB1 is shown as SEQ ID NO: 1, and the coded amino acid sequence of the transcription factor CsMYB1 is shown as SEQ ID NO: 2. Functional tests show that the transcription factor CsMYB1 can interact with a THCA synthetase (THCAS) gene promoter, so that an inhibition effect is realized in the synthesis of cannabinoid compounds; and the regulation and control effect on the synthesis of the cannabinoid compounds can be effectively realized.

The invention provides a transcription factor CsWRKY1 and application thereof and belongs to the technical field of plant genetic engineering. According to the invention, the transcription factor CsWRKY1 is separated from glandular hairs of marihuana as a plant for the first time, a nucleotide sequence of the transcription factor CsWRKY1 is shown as SEQ ID NO:1, and an amino acid sequence coded bythe transcription factor CsWRKY1 is shown as SEQ ID NO:2. Shown through functional tests, the transcription factor CsWRKY 1 can interact with a THCA synthetase (THCAS) gene promoter, so that the inhibition effect is exerted in synthesis of cannabinoid compounds, and the control effect on synthesis of the cannabinoid compounds can be effectively realized.

The invention discloses an AaWBC1 gene promoter, which can regulate the specific expression of the AaWBC1 gene in secretory glandular hairs of Artemisia annua. The nucleotide sequence of the promoter is shown in SEQ ID NO.1. At the same time, the invention discloses an AaWBC1 gene promoter and its function verification method and application. The promoter provided by the invention can guide the specific expression of the reporter gene in the secretory glandular trichomes of plants, and is of great significance to the genetic engineering breeding using plant glandular hair tissue to express and produce metabolites.

The invention discloses a specific expression gene of plant glandular hair HD‑9 , its expression vector and its application, aiming at solving the technical problem of lack of regulation means for the development of plant gland hair head secretory cells. The present invention identifies a gene that can regulate the development of glandular hair cells HD‑9 , the overexpression vector and CRISPR / Cas9 vector of the gene were constructed, and the corresponding overexpression lines and gene knockout lines were obtained by transformation respectively. The identification and detection of phenotype and physiological characteristics showed that, HD‑9 Genes can regulate the development of glandular head cells of plant long stalk glandular hairs, which have important application value in regulating and increasing the secretion content of glandular hairs and directional improvement of glandular hair types.

The invention provides a tissue culture method of potentilla glandulosa, which is characterized by comprising the following steps: by taking tender leaves as explants, sterilizing the surfaces of the tender leaves, inoculating the tender leaves into a callus induction culture medium to induce calluses, and inoculating the induced calluses into an adventitious bud differentiation culture medium to differentiate adventitious buds, the callus induction culture medium is MS + NAA (Naphthalene Acetic Acid) 0.1-05mg / L and TDZ (Toluene Disulfide) 0.1-1.0 mg / L, and the adventitious bud differentiation culture medium is MS + NAA 0.5 mg / L and TDZ 0.1-1.0 mg / L. According to the method, the hormone combination of NAA and TDZ is applied in the callus induction stage of the tissue culture of the potentilla plant for the first time, the induction rate of the potentilla leaf callus can reach 90.00% or above through the induction method, the growth state of the callus is good, and obvious buds can be seen on the surface in the later culture stage. By using the callus differentiation medium disclosed by the invention, the differentiation rate can reach 46.67%, adventitious buds grow robustly, and a good foundation can be laid for successful tissue culture of the potentilla chinensis.

The invention relates to a flaking method for simultaneously observing and counting glandular hairs, non-glandular hairs and stomata of folium artemisiae argyi, which can effectively solve the problem that the distribution conditions of the glandular hairs, the non-glandular hairs and the stomata on the lower surface of the folium artemisiae argyi cannot be simultaneously observed. Soaking and fixing in methanol, rinsing with distilled water, transferring into a sodium hypochlorite solution to be bleached to be colorless, rinsing with distilled water, transferring into a tissue transparent agent to be transparent, taking out the material, dyeing with a sarranine solution, performing water sealing, and observing with an optical microscope until non-glandular hair stems and glandular hair are dyed into red; the method has the advantages of being easy to operate, low in cost, small in workload, high in success rate and clear in observation, the technical requirement for operators is not high, and the method is an innovation in the aspect of the film making method.

The invention provides a method for collecting living glandular hairs of plant leaves. The method comprises the following steps: selecting mature plant leaves to obtain plant monolayer epidermal tissues, cutting the living glandular hairs in the plant monolayer epidermal tissues under a laser microdissection system, and collecting and obtaining the living glandular hairs. The invention provides a method for collecting living glandular hairs of plant leaves, which comprises the following steps: separating and cutting living glandular hair structures on mature plant leaves, and completely retaining and collecting the living glandular hair of the plant leaves under the conditions of not damaging cell structures and not adding exogenous chemical reagents. Completeness and specificity of living glandular hair are reserved to a great extent; meanwhile, the method provided by the invention is suitable for various plants, and provides experimental conditions for researches such as exploration of secondary metabolites in plant glandular hairs and related synthesis paths thereof.

The invention provides transcription factor CsWRKY1 and its application, belonging to the technical field of plant genetic engineering. In the present invention, the transcription factor CsWRKY1 is isolated for the first time from the glandular trichomes of the plant Cannabis trichomes, its nucleotide sequence is shown in SEQ ID NO:1, and its encoded amino acid sequence is shown in SEQ ID NO:2. The functional test of the present invention shows that the transcription factor CsWRKY1 can interact with the THCA synthetase (THCAS) gene promoter, thereby exerting an inhibitory effect on the synthesis of cannabinoid compounds, and can effectively realize the regulatory effect on the synthesis of cannabinoid compounds .

The invention relates to a microscopic slide preparation method for observing the characteristics of the lower surface of folium artemisiae argyi, and effectively solves the problem that an optical microscope or a scanning electron microscope is easy to observe the distribution of pores, glandular hairs and non-glandular hairs on the lower surface of the folium artemisiae argyi and the vertical peripheral wall pattern of epidermal cells. Small leaves are taken and rinsed with distilled water to remove residual gum, and part of the leaves are fixed with methanol, dehydrated with anhydrous alcohol, soaked with isoamyl acetate, dried at a critical point of carbon dioxide and sprayed with metal by an ion instrument for scanning electron microscope slide preparation observation; and soaking the other part of small leaves with a mixed solution of sodium hydroxide and sodium hypochlorite, dissociating mesophyll tissues, bleaching, rinsing with clear water, placing on a glass slide, dyeing with toluidine blue O, making a sodium iodide solution transparent, and sealing with water, thereby obtaining the optical microscope lens for observing stomata and epidermal cells by using a common optical microscope or fluorescence microscope. The method is simple to operate, low in cost, small in workload, high in success rate, clear in observation and good in effect.

The invention relates to H genes and H protein for regulating formation of tomato type I glandular hairs and application of the H genes and H protein for regulating the formation of the tomato type Iglandular hairs, and belongs to the technical field of plant genetic engineering. The cDNA nucleotide sequence of the H genes for regulating the formation of the tomato type I glandular hairs providedby the invention is as shown in SEQ ID NO: 1. The H genes provided by the invention can regulate the formation of the tomato type I glandular hairs. The H genes play a significant role in regulatingthe formation of the tomato type I glandular hairs, and can be applied to the aspects of tomato insect resistance, virus resistance, stress resistance and the like.

The invention relates to a fluorescent staining slide preparation method for observing glandular hair and non-glandular hair forms of a plant leaf epidermal hair cover, which can effectively solve the problem of observing the plant epidermal hair cover by using a fluorescence microscope, and comprises the following steps of: ultrasonically cleaning leaves of a fresh and healthy plant by using distilled water, cutting into small blocks, and immersing in a mixed solution of alcohol and glacial acetic acid; the method comprises the following steps of: rinsing with distilled water, standing in a phloroglucinol solution, taking out a leaf, putting the leaf on a glass slide with one surface with epidermal hair upward, dropwise adding a drop of dilute glycerol above the leaf, respectively exciting with blue light or green light under a fluorescence microscopy, observing that glandular hair and non-glandular hair emit bright yellow fluorescence, and determining that the glandular hair and non-glandular hair emit yellow fluorescence. The fluorescent staining sheet for observing the forms of the glandular hair and the non-glandular hair of the plant leaf epidermis is obtained. The method disclosed by the invention is scientific and reasonable, simple to operate, low in cost, small in workload, high in success rate, clear in observation and good in effect, and is an innovation of a fluorescent staining slide preparation method for microscopically observing plant leaf epidermal hair glandular hair and non-glandular hair forms of folium artemisiae argyi and artemisia scoparia.

The invention provides a fluorescence microscopic slide preparation technology for observing and counting glandular hair and non-glandular hair of folium artemisiae argyi, and relates to the technical field of plant microscopic identification. According to the method, the distribution condition of glandular hairs and non-glandular hairs can be clearly observed through fluorescent staining by utilizing the autofluorescence difference of the glandular hairs and the non-glandular hairs, and the observation and counting of the glandular hairs and the non-glandular hairs are respectively completed. The counting method provided by the invention has the advantages of small technical sampling amount, reagent saving, low cost, easiness in operation and high success rate, can easily achieve a satisfactory experimental purpose, and solves the major scientific problem which puzzles researchers for a long time.