38 results about "Glandularia" patented technology

The invention relates to the technical field of gene engineering, in particular to an application of GRAS11 to regulation of synthesis of plant terpenoids and/or development of glandular hairs. According to the invention, firstly, amplification is performed in Solanumlycopersicum L. to obtain a transcription factor GRAS11, and the transcription factor GRAS11 is specifically expressed in glandular hairs of leaves and stems. The overexpression of GRAS11 can significantly enhance the expression level of terpene synthase and upstream precursor synthetic pathway genes in tissues such as leaves, thereby improving the yield of terpenoids and increasing the volume of glandular hairs. The invention provides beneficial values for research and application in aspects of improving crop nutritional quality, improving crop insect resistance and disease resistance and the like in genetic engineering.

The invention relates to an artemisia apiacea translocator AaPDR3 and an application thereof. The amino acid sequence is shown as SEQ ID No.2; the artemisia apiacea translocator AaPDR3 can affect the synthesizing of sesquiterpenes in artemisia apiacea non-secreting type glandular hair; the translocator is encoded by the nucleotide sequence shown as SEQ ID No.1. The invention further provides a method for realizing transgenosis artemisia apiacea seedlings with the artemisia apiacea translocator AaPDR3.

The invention discloses a method for increasing the aroma content of tobacco leaves and the tobacco leaves with high aroma content, belongs to the technical field of agriculture, and solves the problem that a method for effectively improving aroma content of tobacco leaves by spraying a glandular hair extract and a monopotassium phosphate solution with a certain proportion concentration before tobacco leaves are baked and the problem that the tobacco leaves with high aroma content are obtained after the tobacco leaves are baked by adopting the method are lacked in the prior art. The method mainly comprises the steps: applying fermented soybeans during tobacco transplanting; before tobacco leaves are baked, spraying a glandular hair extract and a monopotassium phosphate solution. The methodis mainly used for increasing the content of aroma substances in the tobacco leaves.

The invention discloses application of a clematis terniflora isopentenyl transferase PT1 gene, an overexpressed arabidopsis strain thereof and a construction method of the overexpressed arabidopsis strain. The nucleotide sequence of the clematis terniflora isopentenyl transferase PT1 gene is as shown in SEQ ID NO. 1. A model plant arabidopsis thaliana is adopted, and a positive strain of arabidopsis thaliana trans-PT1 gene is obtained by constructing a plant PT1 gene overexpression vector and transgenosis operation. The invention proves that the plant morphology of the transgenic arabidopsis thaliana strain has the characteristics of multiple glandular hair on the leaf surface and dark leaf color. After ultraviolet stress, the O2 <-> generation rate and H2O2 content increase in the transgenic arabidopsis thaliana leaves are remarkably lower than those of wild arabidopsis thaliana, the MDA content increase in the leaves is remarkably lower than those of the wild arabidopsis thaliana leaves, and the leaf conductivity increase is remarkably lower than that of the wild arabidopsis thaliana leaves. After extreme ultraviolet stress, the survival rate of the transgenic arabidopsis thaliana is remarkably higher than that of wild arabidopsis thaliana.

The invention relates to a microscopic slide preparation method for observing the characteristics of the lower epidermis of folium artemisiae argyi, which effectively solves the problem of observing the pore of the lower epidermis of folium artemisiae argyi, the vertical peripheral wall pattern of epidermis cells and the distribution of glandular hair and non-glandular hair by using an optical microscope. Rinsing with distilled water, soaking with a mixed solution of sodium hydroxide and hydrogen peroxide, dissociating mesophyll tissues, bleaching, transferring into alcohol, and removing non-glandular hairs on leaf epidermis; the preparation method comprises the following steps of: adding the raw materials into a mixed solution of chromic acid and nitric acid, standing, dissociating mesophyll tissues, rinsing by using alcohol, separating upper and lower epidermis, removing mesophyll tissues of inner epidermis, dyeing the upper and lower epidermis by using a toluidine blue O solution, and rinsing by using water to remove floating color and water seal sheets, thereby obtaining the high-purity mesophyll tissue. The preparation method is simple to operate, low in cost, small in workload, high in success rate, clear in observation and good in effect; the technical problem of observing the lower epidermis of the folium artemisiae argyi is solved, the technical requirement on operators is not high, and an ideal effect can be achieved.

The invention relates to a method for observing epidermal hair of a plant leaf by using a fluorescence microscope. The method can effectively solve the problems of clearly observing the type and distribution of the epidermal hair of the plant and ensuring the quality of medicinal materials by using the fluorescence microscope, and comprises the following steps of: cutting off mature leaves from aplant, cutting off 5-10 mm<2> leaves without thick veins on two sides of the middle section of the middle vein of each leaf, putting the cut leaves into methanol, removing chlorophyll by using ethanol, soaking in berberine, treating by using vanillin-concentrated hydrochloric acid, rinsing, sealing, observing the types and distribution of glandular hairs and non-glandular hairs by using an epifluorescence microscope, and shooting. The method is simple to operate, low in cost, small in workload, high in success rate, clear in observation and good in effect, can completely solve the problem of observation of various plants with leaf epidermis densely covered by non-glandular hairs and glandular hairs, can achieve an ideal effect by not having good requirements on an operation technology, isan innovation in plant structure observation, and has very strong practicability and remarkable economic and social benefits.

The invention provides a transcription factor CsWRKY1 and application thereof and belongs to the technical field of plant genetic engineering. According to the invention, the transcription factor CsWRKY1 is separated from glandular hairs of marihuana as a plant for the first time, a nucleotide sequence of the transcription factor CsWRKY1 is shown as SEQ ID NO:1, and an amino acid sequence coded bythe transcription factor CsWRKY1 is shown as SEQ ID NO:2. Shown through functional tests, the transcription factor CsWRKY 1 can interact with a THCA synthetase (THCAS) gene promoter, so that the inhibition effect is exerted in synthesis of cannabinoid compounds, and the control effect on synthesis of the cannabinoid compounds can be effectively realized.

The invention provides a tissue culture method of potentilla glandulosa, which is characterized by comprising the following steps: by taking tender leaves as explants, sterilizing the surfaces of the tender leaves, inoculating the tender leaves into a callus induction culture medium to induce calluses, and inoculating the induced calluses into an adventitious bud differentiation culture medium to differentiate adventitious buds, the callus induction culture medium is MS + NAA (Naphthalene Acetic Acid) 0.1-05mg/L and TDZ (Toluene Disulfide) 0.1-1.0 mg/L, and the adventitious bud differentiation culture medium is MS + NAA 0.5 mg/L and TDZ 0.1-1.0 mg/L. According to the method, the hormone combination of NAA and TDZ is applied in the callus induction stage of the tissue culture of the potentilla plant for the first time, the induction rate of the potentilla leaf callus can reach 90.00% or above through the induction method, the growth state of the callus is good, and obvious buds can be seen on the surface in the later culture stage. By using the callus differentiation medium disclosed by the invention, the differentiation rate can reach 46.67%, adventitious buds grow robustly, and a good foundation can be laid for successful tissue culture of the potentilla chinensis.

The invention relates to a fluorescent staining slide preparation method for observing glandular hair and non-glandular hair forms of a plant leaf epidermal hair cover, which can effectively solve the problem of observing the plant epidermal hair cover by using a fluorescence microscope, and comprises the following steps of: ultrasonically cleaning leaves of a fresh and healthy plant by using distilled water, cutting into small blocks, and immersing in a mixed solution of alcohol and glacial acetic acid; the method comprises the following steps of: rinsing with distilled water, standing in a phloroglucinol solution, taking out a leaf, putting the leaf on a glass slide with one surface with epidermal hair upward, dropwise adding a drop of dilute glycerol above the leaf, respectively exciting with blue light or green light under a fluorescence microscopy, observing that glandular hair and non-glandular hair emit bright yellow fluorescence, and determining that the glandular hair and non-glandular hair emit yellow fluorescence. The fluorescent staining sheet for observing the forms of the glandular hair and the non-glandular hair of the plant leaf epidermis is obtained. The method disclosed by the invention is scientific and reasonable, simple to operate, low in cost, small in workload, high in success rate, clear in observation and good in effect, and is an innovation of a fluorescent staining slide preparation method for microscopically observing plant leaf epidermal hair glandular hair and non-glandular hair forms of folium artemisiae argyi and artemisia scoparia.

The invention provides a fluorescence microscopic slide preparation technology for observing and counting glandular hair and non-glandular hair of folium artemisiae argyi, and relates to the technical field of plant microscopic identification. According to the method, the distribution condition of glandular hairs and non-glandular hairs can be clearly observed through fluorescent staining by utilizing the autofluorescence difference of the glandular hairs and the non-glandular hairs, and the observation and counting of the glandular hairs and the non-glandular hairs are respectively completed. The counting method provided by the invention has the advantages of small technical sampling amount, reagent saving, low cost, easiness in operation and high success rate, can easily achieve a satisfactory experimental purpose, and solves the major scientific problem which puzzles researchers for a long time.