8 results about "Glandularia" patented technology

The application provides a method for observing glandular hairs of Ainsliaea sinensis by using a scanning electron microscope. The method comprises the following steps: (1) selecting a region to be measured in a leaf blade, cutting a sample, and immersing the sample in an aldehyde fixing solution for fixation and immersion; (2) performing gradient dehydration by using an ethanol solution, performing replacement by using isopentyl acetate, and then performing drying by using a critical point drying instrument; (3) removing non-glandular hairs by using adhesive tape; and (4) then spraying gold and observing under a scanning electron microscope. By using the method, the observation and distribution density statistics of the glandular hairs of Ainsliaea sinensis can be simply, efficiently and accurately completed. By using the method, the problem of uneven leaf blade is well solved, and the problem of non-glandular hairs shielding and interfering with the visual field is completely solved.

The present invention relates to polynucleotide comprising a lncRNA gene according to SEQ ID No: 2, or according to a sequence having at least 50% sequence identity to SEQ ID No: 2, and an AP2c gene according to SEQ ID No: 3, or according to a sequence having at least 50% sequence identity to SEQ ID No: 3, which polynucleotide confers an increased density of type IV trichomes. The invention further relates to a plant comprising said polynucleotide and a SlASAT4 gene according to SEQ ID No. 21, or comprising a homologous sequence having at least 99.6% sequence identity to SEQ ID No. 21 wherein the presence of the polynucleotide leads to increased density of type IV trichomes and wherein the presence of said SlASAT4 gene leads to an increased ratio of S4:17 / S3:15 acyl sugars produced in glandular trichomes present on said plant when compared to a control plant lacking the polynucleotide and the SlASAT4 gene. Such a plant also was found to be resistant to Bemisia tabaci. The invention also discloses markers for the identification of the polynucleotide and the SlASAT4 gene and their use for the identification and development of Solanum lycopersicum plants. Also methods for identifying, producing and selecting of plants and seed are disclosed.

Glandular trichomes are epidermal outgrowths in plants that are the site of metabolic compound synthesis and storage. Modulating the secretion of metabolite compounds in tobacco and tea leaves can alter the taste profile in products made from these plants. The present disclosure relates to methods for modulating secretion from glandular trichomes and compositions comprising altered trichome secretion of metabolites.

The application relates to the technical field of plant molecular biology, and particularly discloses a tomato gland-type epidermal hair regulating gene SlWRKY42 and application thereof, wherein the ORF sequence of the gene SlWRKY42 is shown as SEQ ID NO. 1. By regulating the overexpression of the SlWRKY42 gene in a tomato plant, the density of tomato gland hairs is significantly increased, the transcription and expression of an eneyterpene synthase gene (SlTPS12) and a precursor synthesis pathway transgene are significantly up-regulated, and the content of eneyterpene substances is significantly increased. The gene SlWRKY42 provided by the application can regulate the growth and development of tomato gland-type epidermal hairs, and simultaneously regulate the synthesis of eneyterpene substances.

An electrostatic separation apparatus and method are provided for isolating glandular trichomes from trichome-bearing plant biomass such as cannabis, hemp, or hops. The system may include a vertically oriented particle transport assembly configured to convey a sample through a linear free-fall pathway, a separation chamber with opposing electrode assemblies generating a uniform electrostatic field, and discharge electrodes positioned below the chamber to neutralize residual charge. Airflow regulation may be achieved through laminar diffuser plates and periodic vibration of critical components, including the hopper and diffuser, to prevent clogging and maintain uniform flow. The hopper may further include a circular discharge spout and rounded internal fillets to prevent bridging and ensure consistent powder recirculation. The apparatus achieves high-purity trichome separation through controlled field exposure, aerodynamic stabilization, and charge-neutralized particle discharge, enabling continuous, high-throughput operation with improved yield, reduced maintenance, and enhanced reproducibility across successive processing cycles.

This invention discloses a gene regulating plant glandular trichome development. NtJAZ11, This application aims to address the current technical problem of unclear developmental mechanisms and lack of effective regulatory methods and utilization pathways for glandular trichomes and protective trichomes in plants (such as tobacco). The gene encoding this gene, its expression vector, and its applications are described. NtJAZ11 Overexpression and gene-editing vectors were constructed, and corresponding overexpression and gene-editing lines were obtained through transformation. Subcellular localization, tissue expression, MeJA response pattern analysis, and plant phenotypic identification showed that... NtJAZ11‑2 It can negatively regulate the development of tobacco glandular hairs, and has important application value in regulating the development of plant epidermal hairs.

This invention application discloses a gene regulating plant glandular trichomes. NtMIXTA This application relates to genes, their encoded proteins, biological materials, and applications, aiming to address the technical problem of low efficiency in increasing plant glandular trichome density using traditional breeding techniques. This application clones a gene from tobacco... NtMIXTA Genes, and constructed separately NtMIXTA Gene knockout and overexpression lines were found NtMIXTA Gene knockout reduced leaf glandular hair density, while overexpression lines showed a significant increase in leaf glandular hair density, indicating that... NtMIXTA Genes positively regulate the occurrence and development of glandular trichomes in tobacco. This application is of great significance for studying the molecular regulatory network of plant glandular trichome formation.

The invention particularly discloses a method for inducing transdifferentiation of paving cells in mature leaves of nicotiana benthamiana into glandular hair cells, which comprises the following steps: preparing an agrobacterium infection solution, and agrobacterium is selected from one or more of a GV3101 strain, a C58C1 strain or a K599 strain; carrying out infection treatment on completely expanded mature leaves of the nicotiana benthamiana by using an agrobacterium infection solution; and after infection, the nicotiana benthamiana is cultured, and the paving cells of the leaves are induced to be transdifferentiated into glandular hair cells. The method is easy to operate and good in repeatability, stable genetic transformation and strict sterile conditions are not needed, the paving cells of the Bensi tobacco leaves can be induced to be transdifferentiated into the glandular hair cells, and increase of the number of the glandular hair cells is promoted.