GS microbial inoculum for disposing life wastewater and application of GS microbial inoculum
A domestic sewage and bacterial agent technology, applied in biological water/sewage treatment, water/sludge/sewage treatment, bacteria, etc.
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Embodiment 1
[0046] Prepare culture media according to the culture media of Bacillus, lactic acid bacteria and nitrifying bacteria, and sterilize them at 121°C for 20 minutes. For Bacillus, Lactobacillus casei, Lactobacillus brucei-like and nitrifying bacteria, inoculate the inoculated liquid culture medium except for the nitrifying bacteria at 40°C, and cultivate other bacteria in a shaking table at 37°C for 30-36h, and the rotating speed of the shaking table is 120r / min, so that the strain is in the late logarithmic growth period, collect the bacterial liquid, centrifuge at 5000r / min for 10 minutes, discard the supernatant, take the precipitate, add 20% sterile glycerol, mix well and freeze-dry.
[0047] Methylotrophic Bacillus, Bacillus subtilis, and Bacillus amyloliquefaciens were mixed at a ratio of 1:1:1 as Bacillus, and Lactobacillus casei and Lactobacillus brucei were mixed at a ratio of 1:1 as lactic acid bacteria. Take 24 parts of nitrifying bacteria, 9 parts of Bacillus, and 24...
Embodiment 2
[0049] Prepare culture media according to the culture media of Bacillus, lactic acid bacteria and nitrifying bacteria, and sterilize them at 121°C for 20 minutes. For Bacillus, Lactobacillus casei, Lactobacillus brucei-like and nitrifying bacteria, inoculate the inoculated liquid culture medium except for the nitrifying bacteria at 40°C, and cultivate other bacteria in a shaking table at 37°C for 30-36h, and the rotating speed of the shaking table is 120r / min, so that the strain is in the late logarithmic growth period, collect the bacterial liquid, centrifuge at 5000r / min for 10 minutes, discard the supernatant, take the precipitate, add 20% sterile glycerol, mix well and freeze-dry.
[0050] Methylotrophic Bacillus, Bacillus subtilis, and Bacillus amyloliquefaciens were mixed at a ratio of 1:1:1 as Bacillus, and Lactobacillus casei and Lactobacillus brucei were mixed at a ratio of 1:1 as lactic acid bacteria. Take 24 parts of nitrifying bacteria, 6 parts of bacillus, and 36...
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