Method and system for preparing lipid metabolism type obese gene individualized intervention drink
A technology of lipid metabolism and obesity gene, which is applied in the fields of biochemical equipment and methods, food preparation, determination/inspection of microorganisms, etc.
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Embodiment 1
[0073] Example 1 Establishment of combined genotype risk level one
[0074] The polymorphic site detected in this example is: rs2230806 ( ABCA1 ), rs2241766( ADIPOQ ) and rs320 ( LPL ), label them as a, b, and c, respectively, and their subtype risks are shown in Table 8 below:
[0075] Table 8
[0076] genetic polymorphism genotype Risk (OR) a 1(AA) 1
[0077] The risk level calculated according to the single gene risk level listed in Table 8 is shown in Table 4 above.
Embodiment 2
[0078] Example 2 Establishment of combined genotype risk level II
[0079] The polymorphic site detected in this example is: rs2230806 ( ABCA1 ), rs2241766( ADIPOQ ), rs320( LPL ) and rs429358 ( APOE ), mark them as a, b, c, and d, respectively, and their subtype risks are shown in Table 9 below:
[0080] Table 9
[0081] genetic polymorphism genotype Risk (OR) a 1(AA) 1
2(AG) 1
3(GG) 4.12 b 1(GG) 3.85
2(GT) 1
3(TT) 1 c 1(GG) 1
2(GT) 1
3(TT) 3.22 d 1(CC) 2.69
2(CT) 2.69
3(TT) 1
[0082] The risk level calculated according to the single gene risk level listed in the above table is shown in Table 10 below:
[0083] Table 10
[0084]
Embodiment 3
[0085] Example 3 Individual gene detection one
[0086] The three polymorphic sites in Example 1 were used for individual gene detection.
[0087] Individual oral epithelial cell samples were collected, and the genomic DNA was extracted by silica gel adsorption. After verification by electrophoresis, fluorescence quantitative PCR was performed.
[0088] Individual samples were put into 3 reaction wells, and 3 sites were detected at the same time, namely rs2230806 ( ABCA1 ), rs2241766( ADIPOQ ), rs320( LPL ), in addition, according to the needs of the experiment, an NTC blank control hole without DNA template was added;
[0089] Reagents were added to each reaction well as a fluorescent quantitative PCR reaction system, with a total volume of 10 μL, that is, 2 μL of DNA template with a concentration of 20 ng / μL, 10× fluorescent quantitative PCR reaction buffer ABITaqman ? 1 μL of MGB, 25 mM dNTPs for DNA synthesis of four deoxynucleotide substrates 0.1 μL, 25 mM MgCl 2 ...
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