Application of schisandrin B in preparation of diabetes treatment drug

A technology of schisandra B and a diabetes drug, applied in the field of medicine, can solve the problems such as no report on the anti-diabetic effect, and achieve the effect of reducing the blood sugar level

Inactive Publication Date: 2015-12-23
ZHEJIANG UNIV
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Schisandra chinensis is contained in many traditional Chinese medicines for treating diabetes. Some studies have shown that the extract of schisandra chinen

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of schisandrin B in preparation of diabetes treatment drug
  • Application of schisandrin B in preparation of diabetes treatment drug
  • Application of schisandrin B in preparation of diabetes treatment drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Study on Hypoglycemic Activity of Schizandrin B on Insulin Resistant Hepatocytes

[0025] Mouse embryonic liver cells BNLCL.2 were cultured in low-glucose DMEM containing 10% fetal calf serum at 37°C and 5% CO 2 Incubate in a cell culture incubator and replace with fresh medium every other day. When the cells grow to 80-90%, discard the culture medium, add 3 mL of PBS to rinse once, add 1 mL of 0.05% trypsin-EDTA solution to digest for about 2 minutes, add three times the amount of culture liquid to stop digestion, and incubate the cell solution at 900 r / min Centrifuge for 5 minutes, discard the supernatant, add a certain amount of culture medium, blow off the cells, take 10 μL for counting, inoculate BNLCL.2 cells in a 96-well plate with 20,000 cells per well, and set a cell-free blank control well. After culturing for 24 hours, the original medium was discarded, washed once with phosphate buffered saline (PBS), replaced with low-sugar DMEM culture medium without seru...

Embodiment 2

[0030] Study on Hypoglycemic Activity of Schizandrin B on Insulin Resistant Hepatocytes

[0031] Mouse embryonic liver cells BNLCL.2 were cultured in low-glucose DMEM containing 10% fetal calf serum at 37°C and 5% CO 2 Incubate in a cell culture incubator and replace with fresh medium every other day. When the cells grow to 80-90%, discard the culture medium, add 3 mL of PBS to rinse once, add 1 mL of 0.05% trypsin-EDTA solution to digest for about 2 minutes, add three times the amount of culture liquid to stop digestion, and incubate the cell solution at 900 r / min Centrifuge for 5 minutes, discard the supernatant, add a certain amount of culture medium, blow off the cells, take 10 μL for counting, inoculate BNLCL.2 cells in a 96-well plate with 20,000 cells per well, and set a cell-free blank control well. After culturing for 24 hours, the original medium was discarded, washed once with phosphate buffered saline (PBS), replaced with low-sugar DMEM culture medium without seru...

Embodiment 3

[0035] Study on Hypoglycemic Activity of Schizandrin B on Insulin Resistant Hepatocytes

[0036] Mouse embryonic liver cells BNLCL.2 were cultured in low-glucose DMEM containing 10% fetal calf serum at 37°C and 5% CO 2 Incubate in a cell culture incubator and replace with fresh medium every other day. When the cells grow to 80-90%, discard the culture medium, add 3 mL of PBS to rinse once, add 1 mL of 0.05% trypsin-EDTA solution to digest for about 2 minutes, add three times the amount of culture liquid to stop digestion, and incubate the cell solution at 900 r / min Centrifuge for 5 minutes, discard the supernatant, add a certain amount of culture medium, blow off the cells, take 10 μL for counting, inoculate BNLCL.2 cells in a 96-well plate with 20,000 cells per well, and set a cell-free blank control well. After culturing for 24 hours, the original medium was discarded, washed once with phosphate buffered saline (PBS), replaced with low-sugar DMEM culture medium without seru...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an application of schisandrin B in preparation of a diabetes treatment drug and provides pharmaceutical composition taking the schisandrin B as the effective component. The pharmaceutical composition can effectively reduce the blood glucose level of a diabetic patient, and the effective action concentration is far lower than metformin which is a first-line drug in the market.

Description

technical field [0001] The invention belongs to the technical field of medicine, in particular relates to a medicine for preventing and treating diabetes, in particular to the application of Schizandrin B in the preparation of medicine for treating diabetes. Background technique [0002] According to the statistics of the International Diabetes Federation (International Diabetes Federation, IDF), there were 387 million diabetics in the world in 2014, and the global incidence rate was 8.3%. It is estimated that by 2035, the number of diabetics in the world will reach 592 million. The Diabetes Branch of the Chinese Medical Association conducted a diabetes epidemiological survey in some areas of my country from 2007 to 2008, showing that the prevalence of diabetes among people over 20 years old in my country was 9.7%, and the proportion of prediabetes was 15.5%. Diabetes is a chronic disease. With the prolongation of the disease course, various metabolic disorders can lead to c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K31/36A61P3/10
Inventor 程翼宇王毅范骁辉
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap