Method for application of artificially-cultured vaginal flora transplantation in treating gynecological inflammation

Abstract

The invention discloses a method for application of artificially-cultured vaginal flora transplantation in treating gynecological inflammation. According to the method, feminine dysbacteriosis is recovered by means of the opsonization of normal feminine vagina probiotics florae on feminine vaginal diseases, so that the feminine urogenital tract health is maintained. The method is characterized in that bacteria are used as an antibacterial agent, and the micro-ecology treatment method free of drug resistance and side effects is realized by maintaining the stability of feminine lower genital tract florae; the adopted preparation is a vaginal embolism which is easy to make, convenient to use and quite low in cost.

Description

technical field

[0001] The invention relates to an application method of artificially cultured vaginal flora transplantation in gynecological inflammation, and realizes the treatment and protection of female lower reproductive tract diseases through the method of treating bacteria with bacteria. Background technique

[0002] Many microbial flora often co-exist in the cavity between the human body surface and the outside world, and they play an important physiological role beneficial to the host. The normal microbiota, host and environment are always in a state of dynamic balance, forming an interdependent and mutually restrictive system. Once this balance is disrupted, it may cause disease.

[0003] The female lower reproductive tract is an important microecological zone of the human body. In the vaginal discharge of healthy women, the ratio of anaerobic bacteria to aerobic bacteria is 5:1, and the number of viable bacteria is 10 2 ~10 9 / ml, the number of bacteria can re...

Examples

Embodiment Construction

[0015] The application method of the present invention comprises the following steps:

[0016] 1. Separation of vaginal probiotics: collect excrement samples from the lower genital tract of women in the healthy group, and continue the culture system through N 2 To maintain anaerobic conditions, the fermenter was placed in a 37°C water bath, the pH in the tank was controlled online to 6.5, the operating volume was 500mL, and the dilution rate was pumped in and out at a rate of 35mL / h. After the chemostat stabilized, inoculate the fecal samples. Count viable bacteria every 3 days, take fresh culture solution every day, and store at -80°C. Separation of probiotics using plate separation technology;

[0017] The probiotics are: Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium animalis, Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium longum, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus crispatus, Lactobacillus delbrueckii, ...

technical field

[0001] The invention relates to an application method of artificially cultured vaginal flora transplantation in gynecological inflammation, and realizes the treatment and protection of female lower reproductive tract diseases through the method of treating bacteria with bacteria. Background technique

[0002] Many microbial flora often co-exist in the cavity between the human body surface and the outside world, and they play an important physiological role beneficial to the host. The normal microbiota, host and environment are always in a state of dynamic balance, forming an interdependent and mutually restrictive system. Once this balance is disrupted, it may cause disease.

[0003] The female lower reproductive tract is an important microecological zone of the human body. In the vaginal discharge of healthy women, the ratio of anaerobic bacteria to aerobic bacteria is 5:1, and the number of viable bacteria is 10 2 ~10 9 / ml, the number of bacteria can re...

Examples

Embodiment Construction

[0015] The application method of the present invention comprises the following steps:

[0016] 1. Separation of vaginal probiotics: collect excrement samples from the lower genital tract of women in the healthy group, and continue the culture system through N 2 To maintain anaerobic conditions, the fermenter was placed in a 37°C water bath, the pH in the tank was controlled online to 6.5, the operating volume was 500mL, and the dilution rate was pumped in and out at a rate of 35mL / h. After the chemostat stabilized, inoculate the fecal samples. Count viable bacteria every 3 days, take fresh culture solution every day, and store at -80°C. Separation of probiotics using plate separation technology;

[0017] The probiotics are: Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium animalis, Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium longum, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus crispatus, Lactobacillus delbrueckii, ...