Efficient crucifer club root biocontrol starch bacillus amyloliquefaciens Bam22

A technology for dissolving starch spores and cruciferous families, applied in the directions of bacteria, microorganisms, microorganism-based methods, etc., can solve the problems of poor control effect of biocontrol strains, unfavorable soil flora balance, unsatisfactory control effect, etc. Inhibitory effect, effect of low administration concentration, low dose administration

Active Publication Date: 2016-01-06
INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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Problems solved by technology

[0005] However, although some progress has been made in the research of biocontrol strains, there are still at least three problems: (1) The control effect of biocontrol strains alone is not good, and they often need to be used in combination with chemical agents, which limits The further application of biocontrol strains in the prevention and treatment of cruciferous clubroot; (2) The large application dose is not conducive to the balance of soil flora. If it is used for a lon

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  • Efficient crucifer club root biocontrol starch bacillus amyloliquefaciens Bam22
  • Efficient crucifer club root biocontrol starch bacillus amyloliquefaciens Bam22
  • Efficient crucifer club root biocontrol starch bacillus amyloliquefaciens Bam22

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[0016] Example 1

[0017] Experiment on the control effect of Bacillus amyloliquefaciens Bam22 on cruciferous root disease

[0018] Experimental objects: rape, cabbage, broccoli.

[0019] After cultivating Bacillus amyloliquefaciens Bam22 for 48 hours, dilute it by 200 times, 500 times, 800 times, 1000 times, and use root watering method to water 100ml of each crop during transplanting, watering every 7 days, and watering again every 14 days . Note: The concentration of the undiluted bacterial solution is about 6-8×10 9 cfu / ml.

[0020] Condition classification:

[0021] Grade 0: There is no tumor in the root, and the root system is normal;

[0022] Grade 1: 1-30% of the roots have a little tumor and small nodular masses, which are not connected to each other;

[0023] Grade 2: 31-60% of the roots have tumors with large nodular or spherical masses connected to each other;

[0024] Grade 3: 61-100% of the roots have tumors, which swell to form spindle-shaped tumors. Large tumors extend to...

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Abstract

The invention provides efficient crucifer club root biocontrol starch bacillus amyloliquefaciens Bam22. The efficient crucifer club root biocontrol starch bacillus amyloliquefaciens Bam22 is preserved in China General Microbiological Culture Collection Center on September 6, 2015, and a preservation number of the bacillus amyloliquefaciens is CGMCC No.11329. The strain has an efficient disease prevention effect on crucifer club root, has low concentration of applied bacterium liquid and less dosage, and has a very great actual application prospect.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to a high-efficiency cruciferous clubroot biocontrol bacillus amyloliquefaciens Bam22. Background technique [0002] Brassicaceae clubroot is a worldwide disease caused by Plasmodium brassicae infection. It is spreading in Europe, the United States, Canada, Japan and other countries and regions, seriously threatening the production of Brassicaceae crops. [0003] Cruciferous clubroot in China mainly occurs in East China and South China. Since the 1990s, cruciferous clubroot has spread to Sichuan. Today, the economic losses caused by the clubroot disease in Chengdu only in cruciferous crops have reached hundreds of millions of RMB per year. In addition, rape, as an important oil crop, is facing a particularly serious threat. Therefore, it is necessary to find effective methods to prevent and control clubroot in cruciferous plants (especially rapeseed). ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/07
Inventor 黄小琴刘勇张蕾伍文宪周西全刘红雨
Owner INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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