Immunohistochemical staining method and applications of immunohistochemical staining method in cervical tumorous lesion screening

A technology of immunocytochemistry and staining method, which is applied in the field of immunocytochemical staining method and its application in the screening of cervical neoplastic lesions, can solve the problems of false negatives, reduce the per capita cost of tumor screening, etc., and achieve simple steps, Solve the problem of background and non-specific coloring, with good specificity

Inactive Publication Date: 2016-01-13
黄小军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Another object of the present invention is to provide a method for tumor screening using immunocytochemical staining, which can use different targets to screen and detect all cytological specimens of the human body, providing pathologists with an effective It solves the problem of false negative and false positive in cytological screening of tumor lesions, significantly reduces the workload and burden of pathologists, and solves the background and non-specific coloring problems of immunochemical and in situ hybridization staining in cell smears , Significantly reduce the per capita cost of cancer screening

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] An immunocytochemical staining method, comprising the steps of:

[0090] A. When detecting a single target of a single sample:

[0091] A1. Take the preservation solution of the sample cells and add it to the centrifuge tube added with the separation solution, centrifuge, discard the upper waste liquid, and obtain the precipitate containing the sample cells;

[0092] A2. Add the precipitate containing the sample cells prepared in step A1 into the base solution and mix well to obtain a suspension containing the sample cells;

[0093] A3. Take the glass slide, add the suspension containing the sample cells prepared in step A2 to the working area of ​​the glass slide, and dry to obtain a cell smear;

[0094] A4. Take the cell smear prepared in step A3, fix it with fixative, wash the cell smear with gradient alcohol, then add repair solution for antigen retrieval, add blocking solution to block the background, and then perform immunocytochemical staining.

[0095] In the ...

Embodiment 2

[0111] An immunocytochemical staining method, comprising the steps of:

[0112] B. When detecting a single target of multiple samples:

[0113] B1. Take at least two parts of the preservation solution containing different sample cells and add them to the corresponding centrifuge tubes added with the separation solution, centrifuge, discard the upper waste liquid, and obtain at least two parts of the sediment containing different sample cells;

[0114] B2. Add at least two parts of the precipitates containing different sample cells prepared in step B1 to the base solution and mix well to obtain at least two parts of suspensions containing different sample cells;

[0115] B3. Take the slide, draw at least two separate areas on the working area of ​​the slide and number them, add at least two suspensions containing different sample cells prepared in step 2 to the corresponding separate areas, and dry to obtain cell chips;

[0116]B4. Take the cell chip prepared in step B3, fix ...

Embodiment 3

[0133] An immunocytochemical staining method, comprising the steps of:

[0134] C. When detecting multiple targets in a single sample:

[0135] C1. Take the preservation solution of the sample cells and add it to the centrifuge tube added with the separation solution, centrifuge, discard the upper waste liquid, and obtain the precipitate containing the sample cells;

[0136] C2. Add the precipitate containing the sample cells prepared in step C1 into the base solution and mix evenly to obtain a suspension containing the sample cells;

[0137] C3. Take the glass slide, draw and number at least two separate areas on the working area of ​​the glass slide, add the suspension containing the sample cells prepared in step C2 to each separate area, and dry to obtain a cell chip;

[0138] C4. Take the cell chip prepared in step C3, fix it with fixative, wash the cell chip with gradient alcohol, then add repair solution for antigen repair, add antibody or probe, add blocking solution t...

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Abstract

The present invention relates to the technical field of cytologic screening of tumorous lesions, particularly to an immunohistochemical staining method and applications of the immunohistochemical staining method in cervical tumorous lesion screening. The method comprises: obtaining a precipitate containing sample cells; obtaining a suspension containing sample cells; obtaining a cell smear or cell chip; and taking the cell smear or cell chip, immobilizing with an immobilize liquid, carrying out water washing on the cell chip with gradient alcohol, adding a repair liquid to carry out antigen repair, adding antibody or probe, adding a blocking solution to block the background, and carrying out immunohistochemical staining. According to the present invention, the false negative and false positive problem of the tumorous lesion cytologic screening can be solved, the workload and the burden of the pathologists can be significantly reduced, the immunochemistry and in situ hybridization staining background and non-specific coloring problem in the cell smear can be solved, and the per capita cost of the tumor screening can be significantly reduced.

Description

technical field [0001] The invention relates to the technical field of cytological screening of neoplastic lesions, in particular to an immunocytochemical staining method and its application in screening of cervical neoplastic lesions. Background technique [0002] Cytological screening and diagnosis of neoplastic lesions can detect some malignant tumors early, and the now popular liquid-based cytological examination of the cervix has effectively reduced the incidence and mortality of cervical cancer. However, the liquid-based cytology based on Papanicolaou staining has its natural defects: (1) In cervical biopsy, there are still some lesions that rely on the detection of P16 and Ki67 to distinguish whether they are high-grade lesions (HSILE) or the response to inflammation. In this part of the cases, Pap staining cytology is helpless; (2) Improper collection of materials, unqualified, and too few diseased cells in the specimens sent for inspection; (3) Gene levels have chan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N33/574
Inventor 黄小军史健
Owner 黄小军
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