Preparation method of cell chip and application of cell chip in tumour screening field

Abstract

The invention relates to the technical field of cell chips and in particular relates to a preparation method of a cell chip and an application of the cell chip in the tumour screening field. The preparation method of the cell chip comprises the following steps: (1) taking preserving fluids containing different sample cells, respectively adding the preserving fluids into corresponding centrifugal pipes added with separating mediums, and centrifuging, so that sediments containing different sample cells are obtained; (2) respectively adding a base fluid into the sediments containing different sample cells, and uniformly mixing, so that suspension liquids containing different sample cells are obtained; and (3) taking a glass slide, drawing separated regions in a working range of the glass slide and numbering, respectively adding the suspension liquids containing different sample cells into corresponding separated regions, and carrying out natural air cooling, so that the cell chip is obtained. The prepared cell chip can be applied to the tumour screening field, and the cell chip can detect two to dozens of samples on one glass slide, staining examination cost can be reduced by multiplied times or dozens of times, and economic burden of the crowd participating in tumour screening can be reduced.

Description

technical field [0001] The invention relates to the technical field of cell chips, in particular to a method for preparing a cell chip and its application in the field of tumor screening. Background technique [0002] Cytological screening and diagnosis of neoplastic lesions can detect some malignant tumors early, and the now popular liquid-based cytological examination of the cervix has effectively reduced the incidence and mortality of cervical cancer. However, liquid-based cytology based on Papanicolaou staining has its natural defects: (1) In cervical biopsy, there are still some lesions that rely on the detection of P16 and Ki67 to distinguish whether they are high-grade lesions (HSIL) or the response to inflammation. In this part of the cases, the Pap staining cytology is powerless; (2) Improper collection of materials, unqualified, too few diseased cells in the sample sent for inspection; (3) The gene level of some cases has changed and has caused P16 to increase, an...

AI Technical Summary

Problems solved by technology

However, liquid-based cytology based on Papanicolaou staining has its natural defects: (1) In cervical biopsy, there are still some lesions that rely on the detection of P16 and Ki67 to distinguish whether they are high-grade lesions (HSIL) or the response to inflammation. In this part of the cases, the Pap staining cytology is powerless; (2) Improper collection of materials, unqualified, too few diseased cells in the sample sent for inspection; (3) The gene level of some cases has changed and has caused P16 to increase, and the cells The cycle is out of control, but the morphology has not changed; (4) The doctor has not received strict long-term training, and the number of film readings has not reached the minimum requirement. There are many diseased cells under the microscope, but the doctor dare not confirm. HSIL has never been issued before; ⑸, the workload of doctors is too heavy, and the fatigue reading for a long time leads to an increase in the missed diagnosis rate; ⑥, the production technology is not qualified, and the shape of cells is artificially changed, and the shape of diseased cells is distorted and unrecognizable

[0009] Cell chip technology is to detect dozens or hundreds of patient samples on a glass slide. Combining with various biological detection technologies, it can detect multiple disease indicators at low cost and high efficiency. Due to non-specific interference and other reasons, it has not been used in clinical practice. Effective application in pathological diagnosis