Telecentric optical structure-based transmission-type digital holographic microscopic imaging device

A digital holographic microscope and optical structure technology, applied in the direction of instruments, can solve the problems of high system stability requirements, phase distortion cannot be completely eliminated, and difficult to determine and adjust the position of the microscope objective lens, so as to reduce complexity and avoid Aberration and distortion, the effect of improving the speed of imaging

Inactive Publication Date: 2016-01-13
NANJING UNIV OF SCI & TECH
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Problems solved by technology

However, the two-step exposure method needs to record two holograms, which requires high system stability
The M.K.Kim research group in the United States proposed a method of physical compensation ([4] M.K.Kim. "Applications of Digital Holography in Biomedical Microscopy". J.Opt.Soc.Korea.2010,14(2):77~89), this method does not Instead of using a tube lens in the light path, another identical microscope objective lens was added to t

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  • Telecentric optical structure-based transmission-type digital holographic microscopic imaging device
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  • Telecentric optical structure-based transmission-type digital holographic microscopic imaging device

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[0010] 1(a), the present invention is based on a telecentric optical structure transmission digital holographic microscopic imaging device, including a laser 1, a first beam splitter 2, a first collecting mirror 3, and a first concentrating mirror pinhole diaphragm 4 , the first condenser lens 5, the first plane mirror 6, the microscope objective lens 8, the lens tube lens 9, the second condenser lens 10, the second condenser mirror pinhole diaphragm 11, the second condenser lens 12, the attenuation sheet 13, the second plane mirror 14 , the second beam splitter 15 and the camera 16, wherein the first beam splitter 2 divides the laser light emitted by the laser 1 into two paths, wherein the laser light of one path converges to the first condenser pinhole diaphragm 4 through the first collector mirror 3, and the light After being diverged by the pinhole diaphragm 4 of the first condenser lens, it is collected by the first condenser lens 5 and becomes parallel light, which is ref...

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Abstract

The invention discloses a telecentric optical structure-based transmission-type digital holographic microscopic imaging device. By a telecentric optical structure, two beams of parallel light, namely object light and reference light intervene on an imaging plane of a camera to form an interference pattern, so that aberration in a conventional digital holographic microscopic imaging device can be avoided; the accuracy of a system is greatly improved; other complicated physical or aberration compensation calculation processes are not needed; the imaging speed is improved; and the late calculating and processing complexity is lowered.

Description

technical field [0001] The invention belongs to optical measurement and imaging technology, in particular to a transmission digital holographic microscopic imaging device based on a telecentric optical structure. Background technique [0002] As a new type of coherent measurement and imaging technology, digital holography has the biggest advantage of being able to simultaneously and independently obtain quantitative amplitude information and phase information of objects. Phase information is particularly important when performing quantitative detection of almost transparent objects such as biological cells. However, when using the traditional digital holographic microscope system for quantitative phase measurement, in order to obtain the phase image of the object accurately, the phase distortion in the reconstructed image must be corrected first, which requires knowing the various parameters in the experiment, such as Record the distance, the included angle of the object pa...

Claims

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Application Information

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IPC IPC(8): G03H1/00G03H1/04
CPCG03H1/00G03H1/0443G03H2001/005
Inventor 陈钱孙佳嵩左超冯世杰顾国华张玉珍胡岩张良陶天阳李加基张佳琳孔富城林飞张敏亮范瑶
Owner NANJING UNIV OF SCI & TECH
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