A technical method for parent matching by using rice F2 generation heterosis

A technology of parental combination and heterosis, applied in the field of hybrid japonica rice genetics and breeding, to achieve the effects of strong operability, clear selection, and good horizontal resistance

Inactive Publication Date: 2016-01-27
MEIHEKOU JIYANG SEED CO LTD
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Problems solved by technology

[0003] Invented a new technology of breeding "japonica rice F2 generation using parental combination". The field performance of the plant is divided into female parent group and male parent group, combined with molecular marker technology to measure the genetic distance and pedigree relationship between the materials, select the appropriate parent pairing group, and test the yield, resistance, uniformity, quality and other traits of the F2 generation Identification, so as to select and breed F2 generation combinations with strong heterosis, good horizontal resistance, and high uniformity, which effectively solves the problems of blind hybridization and low efficiency, and makes the selection of F2 generation parent combinations more targeted

Method used

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Embodiment Construction

[0012] Below in conjunction with embodiment the present invention is further described.

[0013] 1. In the first ten days of September 2011, 153 japonica rice materials with consistent plant and leaf shape (compact), semi-straight panicle and relatively sudden light and temperature were selected from the experimental field (Meihekou City, Jilin Province) as the parental population used by F2.

[0014] 2. In the last ten days of September of that year, 153 parental materials were taken as the standard with a plant height of 105 cm, the female parent group was lower than 105 cm, and the male parent group was higher than 105 cm, and then the female parent group was eliminated. Fewer materials were used to eliminate materials with smaller spikes in the male parent group. After statistics, there were 35 female parent groups and 22 male parent groups, a total of 57 materials.

[0015] 3. In late October of that year, DNA was extracted from 57 japonica rice materials in the laborator...

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Abstract

The invention relates to a technical method for parent matching by using rice F2 generation heterosis, and belongs to the technical field of hybrid rice genetic breeding. The method comprises the following embodiments: (1) selection of parent materials and division of maternal and paternal groups; (2) advanced selection for the hybridized combination by using SSR molecular marks; (3) further screening and combining according to rules that a difference of parent plant heights is no more than 12 cm, a heading stage difference is no more than 3d and the coordination of the two conditions should be taken into consideration, and the stress resistance of the parent material is as complementary as possible, and artificial emasculating and hybridizing; (4) field identification and indoor plant inquisition of F2 groups, and determination of the parent combination; and (5) transformation of parent materials. In the technical method, materials are definitely selected and divided, parent matching mode for F2 generation is selected by combining regular breeding and molecular mark assisted breeding; the technical method has significance to the development of hybrid rice.

Description

Technical field [0001] The patent of the invention belongs to the field of genetic breeding of hybrid japonica rice, and is a technical method for efficient and targeted breeding of a new combination of F2 generation of hybrid japonica rice that can be used, and avoids the difficulty in seed production of F1 generation of hybrid japonica rice. technical background [0002] The promotion and utilization of hybrid japonica rice is facing many problems, among which the low yield, high cost and high risk of seed production are the most important reasons restricting the development of hybrid japonica rice. There are a large number of gene heterozygous individuals in the F2 generation of rice, and the combination potential of the population with high yield and good level of resistance is great, and the self-propagation coefficient of rice is high. Therefore, the utilization of F2 generation is one of the development directions of hybrid japonica rice. one. However, the F2 generat...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
Inventor 马科周圆圆华泽田杨银阁东丽宗景章杨超杨凯
Owner MEIHEKOU JIYANG SEED CO LTD
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