Method for breeding hybrid cabbage type rape through composite pollination system

Abstract

The invention belongs to the technical field of cabbage type rape breeding, and particularly relates to a method for breeding a hybrid cabbage type rape through a composite pollination line. Accordingto the method, in an artificial isolation zone, multiple SI lines are taken as female parents, and CMS maintainer / recovery lines are taken as male parents to prepare SI*CMS maintainer / recovery line F1s; the F1s are screened; the self-compatibility of the F1s is identified, and if the F1s have self-compatibility, the F1s are two-line hybrids of the SI; the F1s with self-incompatibility are taken as female parents, and SI recovery lines are taken as male parents to prepare hybrids, and SI three-line hybrids are obtained; reciprocal cross F1s are prepared; F1s are taken as male parents to prepare hybrids with CMS lines to obtain cytoplasmic male sterile double cross hybrids. The male parents of the CMS three-line hybrids and CMS double cross hybrids do not have self-compatibility and can behybridized and mixed with female parents to prepare hybrids, thereby reducing the seed production cost; the use amount of self-incompatibility materials as the male parents is less than the use amountof the self-incompatibility materials as the female parents, and the problem is solved that the breeding SI lines are contaminated by foreign pollens easily.

Description

technical field [0001] The invention belongs to the technical field of Brassica napus breeding, and in particular relates to a method for breeding Brassica napus hybrids with a compound pollination system. In particular, it relates to a method for breeding hybrids of Brassica napus by compounding two pollination systems of cytoplasmic male sterility and self-incompatibility. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] The utilization of heterosis in Brassica napus includes cytoplasmic male sterility (CMS), nuclear male sterility (GMS), self-incompatibility (SI) and chemical male sterility. At present, Brassica napus Polima Cytoplasmic Male Sterility (Pol CMS) and Radish Cytoplasmic Male Sterility (Ogura CMS) are the most used at home and abroad. Only with cytoplasmic sterility gene and nuclear sterility gene at the same time can they show sterility, and hybrids must carry the restorer gene to se...

AI Technical Summary

Problems solved by technology

[0009] In the prior art, when using the cytoplasmic male sterile pollination system to breed hybrids, it is necessary to select a homogeneous maintainer line to propagate the sterile line, and to breed a restorer line to completely restore the sterility to fertility, and the breeding procedure is complicated , Low breeding efficiency

[0010] (2) The self-incompatibility line of the prior art can be a maintainer line or restorer line of cytoplasmic male sterility, and the maintainer line or restorer line of cytoplasmic male sterility can be a restorer line of self-incompatibility, but there is no Utilization, the resource utilization efficiency of rapeseed is low

[0011] (3) In the prior art, the procedure for breeding hybrids using cytoplasmic male sterility or self-compatibility of Brassica napus is generally as follows: firstly, the sterile line and the restorer line are selected respectively, and then the sterile line and the restorer line are configured Hybrids, the final selection of hybrids, blindness, and it is difficult to obtain strong dominant hybrids

PolCMS and Ogura CMS still have shortcomings such as few restorer sources, long breeding cycle for maintainer lines and restorer lines, and small genetic differences between female parents, male parents, and male and female parents

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