-2786 locus variant of mitochondrial fusion gene 2 and detection method of -2786 locus variant

A site and gene technology, applied in the field of kits for detecting this SNP site, can solve the problems of abnormal expression regulation, less research, and decreased expression, etc.

Inactive Publication Date: 2016-02-24
BEIJING ANZHEN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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Problems solved by technology

[0005] Since the main characteristic of Mfn2 gene in the blood vessels and leukocytes of patients with essential hypertension and spontaneously hypertensive rats is the decreased expression, this should be due to the abnormality of its expression regulation. few

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  • -2786 locus variant of mitochondrial fusion gene 2 and detection method of -2786 locus variant

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Embodiment 1

[0028] The 5'-end non-coding region-2786 locus of essential hypertension susceptibility gene MFn2 gene was detected by sequencing method. The above-mentioned hypertension cases and 30 control samples were selected for sequencing to determine the genotype of the 5'-end non-coding region-2786 site.

[0029] 1. Experimental method

[0030] The PCR sequencing primers still use the above-mentioned PCR primers, and the amplified products are directly sequenced after being purified. The sequencing instrument was ABI's 3130xl genetic analyzer, which was analyzed with sequenceanalysis5.2 analysis software, and the results could also be viewed with chromas.

[0031] 2. Experimental results

[0032] Screenshot of the sequencing result figure 1 shown.

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Abstract

The invention discloses a -2786 locus variant of mitochondrial fusion gene 2 and a detection method of the -2786 locus variant, discloses a single nucleotide locus related to primary hypertension susceptibility and discloses a method for detecting single nucleotide polymorphism of primary hypertension related gene-mitochondrial fusion gene 2 (Mfn2) or hyperplasia suppressor gene (HSG). The method includes: detecting genotype of -2786 locus in a 5' end non-coding region of mitochondrial fusion gene 2 (Mfn2) or hyperplasia suppressor gene (HSG) of an individual. The invention further discloses a corresponding detection kit.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine. More specifically, it involves a single nucleotide polymorphism (Singlenucleotidepolymorphism, SNP) at the 5' non-coding region-2786 position of human mitochondrial fusion gene 2 (Mitofusin2, Mfn2gene) and its association with essential hypertension. detection. The present invention also relates to a kit for detecting this SNP site. Background technique [0002] Essential hypertension (Essential Hypertension, EH) is a common and frequent multi-factor and polygenic disease caused by environmental and genetic factors, which has a great impact on human health. With the development of molecular medicine, more than 150 hypertension-related genes have been discovered, but the pathogenesis of EH is still not fully understood, and the problems of early diagnosis and prevention of hypertension are still not fully resolved. EH is the result of a combination of genetic factors and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 王佐广靳飞李闯李梅彭晓云刘洁琳刘雅牛秋丽孙东东魏永祥温绍君
Owner BEIJING ANZHEN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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