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Evening primrose b-casein phosphopeptide-chitosan nanoparticles and its preparation method and application

A casein phosphopeptide and chitosan nanoparticle technology, which is applied in the fields of application, hydrolyzed protein components, peptide/protein components, etc., can solve the problem of affecting the formation of evening primrose B-casein phosphopeptide-chitosan nanoparticles , not clear and other issues, to achieve significant carrying effect, stable characterization parameters, and good storage stability

Active Publication Date: 2018-12-07
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, how the above factors specifically affect the production of evening primrose B-casein phosphopeptide-chitosan nanoparticles, as well as the optimum range in the preparation of the nanoparticles, have not been clarified before.

Method used

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  • Evening primrose b-casein phosphopeptide-chitosan nanoparticles and its preparation method and application
  • Evening primrose b-casein phosphopeptide-chitosan nanoparticles and its preparation method and application
  • Evening primrose b-casein phosphopeptide-chitosan nanoparticles and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] One, the preparation method of evening primrose B-casein phosphopeptide-chitosan nanoparticles, the specific steps are as follows:

[0051] (1) Selection of materials: CPPs with a purity of 90% (w / w) are polyanion materials, CS with a deacetylation degree of 90% or more and a molecular weight of 130kDa is a polycation material, and OEB with a purity of 90% (w / w) or more , the purity of genipin is 98% (w / w).

[0052] (2) Prepare a certain concentration of CPPs solution, CS solution and OEB solution respectively, and adjust the pH of the three solutions to 2.00, and the NaCl concentration is 1mol / L. In addition, 0.5% (v / v) acetic acid solution was used to assist in dissolving CS, and 1 mol / L HCl was used to adjust the pH of the three solutions.

[0053] (3) Mix the CPPs solution and the OEB solution evenly, and then add them dropwise to the CS solution in a stirring state. The mass fractions of CPPs, CS and OEB in the final solution are 43%, 43% and 14%, respectively. T...

Embodiment 2

[0080] A preparation method of evening primrose B-casein phosphopeptide-chitosan nanoparticles, the specific steps are as follows:

[0081] (1) Selection of materials: CPPs with a purity of 90% (w / w) are polyanion materials, CS with a deacetylation degree of 90% or more and a molecular weight of 130kDa is a polycation material, and OEB with a purity of 90% % (w / w), the purity of genipin is 98% (w / w).

[0082](2) CPPs solution, CS solution and OEB solution with a certain concentration were prepared respectively, and the pH of the three solutions was adjusted to 2.00 and the NaCl concentration was 1 mol / L. In addition, 0.5% (v / v) acetic acid solution was used to assist dissolving CS, and 1 mol / L HCl was used to adjust the pH of the three solutions.

[0083] (3) Mix the CPPs solution and the OEB solution uniformly, and then add dropwise to the CS solution in a stirring state. The mass fractions of CPPs, CS and OEB in the final solution are 43%, 43% and 14%, respectively. The fi...

Embodiment 3

[0089] A preparation method of evening primrose B-casein phosphopeptide-chitosan nanoparticles, the specific steps are as follows:

[0090] (1) Selection of materials: CPPs with a purity of 90% (w / w) are polyanionic materials, CS with a degree of deacetylation of more than 90% and a molecular weight of 44-500 kDa are polycationic materials, and OEB with a purity of 90% (w / w) ) or more, the purity of genipin was 98% (w / w).

[0091] (2) CPPs solution, CS solution and OEB solution with a certain concentration were prepared respectively, and the pH value of the solution was adjusted to 2.00 and the NaCl concentration was 1 mol / L. In addition, 0.5% (v / v) acetic acid solution was used to assist dissolving CS, and 1 mol / L HCl was used to adjust the pH of the three solutions.

[0092] (3) Mix the CPPs solution and the OEB solution uniformly, and then add dropwise to the CS solution in a stirring state. The mass fractions of CPPs, CS and OEB in the final solution are 40%, 40% and 20%,...

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Abstract

The invention discloses an evening essence B-casein phosphopeptide-chitosan nano particle, a preparation method and applications thereof. The preparation method comprises the following steps: preparing a CPPs solution, a CS solution, and an OEB solution, adding three solutions in a certain sequence, evenly mixing, controlling the mass percentage of CPPs and OEB of the final solution and the total concentration of CPPs, CS, and OEB of the solution, adjusting the pH value of the system to obtain nano spheres; then adding genipin to fix the nano spheres to obtain nano suspension, and subjecting the nano suspension to centrifugation and drying to obtain the evening primrose essence B-casein phosphopeptide-chitosan nano particles. The nano particle has the advantages of adjustable particle size, high embedding rate, pH responding property, green, energy saving, safety, stomach acid resistance, and sustain-released function, and can be widely used in the fields such as pharmacy, food, healthcare product, and the like.

Description

technical field [0001] The invention relates to the development of an anti-oxidative water-soluble macrocyclic dimer tannin nanometer sustained-release technology, in particular to evening primrose B-casein phosphopeptide-chitosan nanoparticle and a preparation method and application. Background technique [0002] With the continuous advancement of science and technology and the continuous improvement of people's living standards, human's requirements for food have changed from color, aroma and taste to nutrition and health care. In the research and development boom of functional foods, antioxidant products have attracted much attention because of their unique and effective inhibition of the oxidation reaction of free radicals. A certain amount of free radicals in the human body plays an important role in transferring energy, killing bacteria and parasites, or participating in the elimination of toxins. However, in today's increasingly polluted environment and increasing wo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/55A61K47/61A61K47/64A61K38/01A61K31/722A61K31/7024A61P39/06C08J3/24A23L33/18A23L33/105A61K47/69
CPCA23V2002/00A61K31/7024A61K31/722A61K38/018C08J3/246C08J2305/08A23V2200/30A23V2250/21A23V2250/511A23V2250/54246
Inventor 赵力超曹庸陈运娇曹素芳钟颖儿刘梓韬
Owner SOUTH CHINA AGRI UNIV
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