Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Evening primrose essence B-casein phosphopeptide-chitosan nano particle, preparation method and applications thereof

A casein phosphopeptide and chitosan nanoparticle technology, which is applied in the fields of application, hydrolyzed protein components, peptide/protein components, etc., can solve the problem of affecting the formation of evening primrose B-casein phosphopeptide-chitosan nanoparticles , not clear and other issues, to achieve significant loading effect, stable characterization parameters, and high drug loading effect

Active Publication Date: 2016-03-09
SOUTH CHINA AGRI UNIV
View PDF1 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, how the above factors specifically affect the production of evening primrose B-casein phosphopeptide-chitosan nanoparticles, as well as the optimum range in the preparation of the nanoparticles, have not been clarified before.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Evening primrose essence B-casein phosphopeptide-chitosan nano particle, preparation method and applications thereof
  • Evening primrose essence B-casein phosphopeptide-chitosan nano particle, preparation method and applications thereof
  • Evening primrose essence B-casein phosphopeptide-chitosan nano particle, preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] One, the preparation method of evening primrose B-casein phosphopeptide-chitosan nanoparticles, the specific steps are as follows:

[0051] (1) Selection of materials: CPPs with a purity of 90% (w / w) are polyanion materials, CS with a deacetylation degree of 90% or more and a molecular weight of 130kDa is a polycation material, and OEB with a purity of 90% (w / w) or more , the purity of genipin is 98% (w / w).

[0052] (2) Prepare a certain concentration of CPPs solution, CS solution and OEB solution respectively, and adjust the pH of the three solutions to 2.00, and the NaCl concentration is 1mol / L. In addition, 0.5% (v / v) acetic acid solution was used to assist in dissolving CS, and 1mol / L HCl was used to adjust the pH of the three solutions.

[0053] (3) Mix the CPPs solution and the OEB solution evenly, and then add them dropwise to the CS solution in a stirring state. The mass fractions of CPPs, CS and OEB in the final solution are 43%, 43% and 14%, respectively. Th...

Embodiment 2

[0080] A preparation method of evening primrose B-casein phosphopeptide-chitosan nanoparticles, the specific steps are as follows:

[0081] (1) Selection of materials: CPPs with a purity of 90% (w / w) are polyanion materials, CS with a deacetylation degree of 90% or more and a molecular weight of 130kDa is a polycation material, and OEB with a purity of 90% % (w / w), the purity of genipin is 98% (w / w).

[0082](2) Prepare a certain concentration of CPPs solution, CS solution and OEB solution respectively, and adjust the pH of the three solutions to 2.00, and the NaCl concentration is 1mol / L. In addition, 0.5% (v / v) acetic acid solution was used to assist in dissolving CS, and 1mol / L HCl was used to adjust the pH of the three solutions.

[0083] (3) Mix the CPPs solution and the OEB solution evenly, and then add them dropwise to the CS solution in a stirring state. The mass fractions of CPPs, CS and OEB in the final solution are 43%, 43% and 14%, respectively. The final concent...

Embodiment 3

[0089] A preparation method of evening primrose B-casein phosphopeptide-chitosan nanoparticles, the specific steps are as follows:

[0090] (1) Selection of materials: CPPs with a purity of 90% (w / w) are polyanion materials, CS with a deacetylation degree of more than 90% and a molecular weight of 44 to 500kDa are polycation materials, and OEBs with a purity of 90% (w / w) ) above, the purity of genipin is 98% (w / w).

[0091] (2) Prepare a certain concentration of CPPs solution, CS solution and OEB solution respectively, and adjust the pH value of the solution to 2.00, and the NaCl concentration is 1mol / L. In addition, 0.5% (v / v) acetic acid solution was used to assist in dissolving CS, and 1mol / L HCl was used to adjust the pH of the three solutions.

[0092] (3) Mix the CPPs solution and the OEB solution evenly, and then add them dropwise to the CS solution in a stirring state. The mass fractions of CPPs, CS and OEB in the final solution are 40%, 40% and 20% respectively. The...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses an evening essence B-casein phosphopeptide-chitosan nano particle, a preparation method and applications thereof. The preparation method comprises the following steps: preparing a CPPs solution, a CS solution, and an OEB solution, adding three solutions in a certain sequence, evenly mixing, controlling the mass percentage of CPPs and OEB of the final solution and the total concentration of CPPs, CS, and OEB of the solution, adjusting the pH value of the system to obtain nano spheres; then adding genipin to fix the nano spheres to obtain nano suspension, and subjecting the nano suspension to centrifugation and drying to obtain the evening primrose essence B-casein phosphopeptide-chitosan nano particles. The nano particle has the advantages of adjustable particle size, high embedding rate, pH responding property, green, energy saving, safety, stomach acid resistance, and sustain-released function, and can be widely used in the fields such as pharmacy, food, healthcare product, and the like.

Description

technical field [0001] The invention relates to the development of an antioxidant water-soluble macrocyclic dimer tannin nano-sustained-release technology, in particular to an evening primrose B-casein phosphopeptide-chitosan nanoparticle, a preparation method and application thereof. Background technique [0002] With the continuous advancement of science and technology and the continuous improvement of people's living standards, human requirements for food have changed from color, aroma and taste to nutrition and health care. In the upsurge of research and development of functional foods, antioxidant products have attracted much attention because of their unique ability to effectively inhibit the oxidation reaction of free radicals. A certain amount of free radicals in the human body plays an important role in transferring energy, killing bacteria and parasites, or participating in the elimination of toxins. However, in today's environment with increasing environmental po...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K47/48A61K38/01A61K31/722A61K31/7024A61P39/06C08J3/24A23L33/18A23L33/105A61K47/61A61K47/64A61K47/69
CPCA23V2002/00A61K31/7024A61K31/722A61K38/018C08J3/246C08J2305/08A23V2200/30A23V2250/21A23V2250/511A23V2250/54246
Inventor 赵力超曹庸陈运娇曹素芳钟颖儿刘梓韬
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com