Artificial breeding method of spiny eels
A technology of artificial breeding and stingray, which is applied in fish farming, application, climate change adaptation, etc., can solve the problems of artificial breeding of stingray, and achieve the effects of facilitating promotion, high induced labor rate, and low parental mortality
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Embodiment 1
[0023] 1. Selection of parents
[0024] Ninety-eight sexually mature broodstock were selected from wild-caught eel eel individuals. Females weighing more than 30g have swollen and soft abdomens, and the reproductive holes are round and red. Males weighing more than 50g are strong and flexible, and most of them can squeeze out semen. The ratio of male to female is 1:1.
[0025] 2. Ways to induce labor
[0026] Artificial oxytocin drugs including HCG, LRH-A 2 and DOM. The dosage of oxytocic drugs is shown in Table 1. Artificial oxytocin drugs also include 0.3ml of 0.7% sodium chloride solution per kilogram of broodstock. The method of induction of labor is two injections into the back muscle, the first injection only injects LRH-A 2 , and the injection time interval of the second needle is 12 hours.
[0027] 3. Artificial insemination method
[0028] After 10 hours, check the broodstock every two hours, and squeeze the abdomen lightly until eggs flow out before artificia...
Embodiment 2
[0034] A total of 112 sexually mature broodstock were selected from wild-caught eel eel individuals. Females weighing more than 30g have swollen and soft abdomens, and the reproductive holes are round and red. Males weighing more than 50g are strong and flexible, and most of them can squeeze out semen. The ratio of male to female is 1:1.2. Other steps are the same as in Example 1.
Embodiment 3
[0036] Seventy sexually mature broodstock were selected from wild-caught eel eel individuals. Females weighing more than 30g have swollen and soft abdomens, and the reproductive holes are round and red. Males weighing more than 50g are strong and flexible, and most of them can squeeze out semen. The ratio of male to female is 1:1.1. Other steps are the same as in Example 1.
[0037] Examples 1-3 and the effects of dosage of artificial oxytocin drugs on effect time, parental oxytocin rate, fertilization rate, parental mortality rate, hatching rate and 7-day survival rate after hatching are shown in Table 1.
[0038] Table 1 The effect of oxytocic drug dose on the reproductive effect of the lobster
[0039]
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