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Antler protein extract and its medicinal application

A protein extract and extract technology, applied in the direction of peptide/protein components, biochemical equipment and methods, drug combination, etc., can solve the problem of less protective effect on myocardial injury

Active Publication Date: 2019-12-13
EXPERIMENTAL RES CENT CHINA ACAD OF CHINESE MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the protective effect of velvet protein mixture on myocardial injury is less researched

Method used

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  • Antler protein extract and its medicinal application
  • Antler protein extract and its medicinal application
  • Antler protein extract and its medicinal application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Embodiment 1, preparation and detection of velvet antler protein extract

[0096] 1. Preparation of deer antler protein extract

[0097] Prepare deer antler protein extract according to the following method:

[0098] The fresh velvet antler is directly crushed for the first time with a pulverizer to obtain the first crushed velvet antler. Put the velvet antler crushed for the first time into liquid nitrogen quick-freezing, put it into a superfine pulverizer, add an appropriate amount of water for the second crushing until it is minced meat, and obtain the second crushed velvet antler, the molecular particle size of the second crushed velvet antler is 75 μm. Add a certain amount of water to the second crushed velvet antler (the mass ratio of the second crushed velvet to water is 1:5), then add an appropriate amount of glacial acetic acid to keep the pH at 3.5, keep it at 4°C for 24 hours, and then centrifuge at 4000g for 10 minutes to separate The supernatant is clear...

Embodiment 2

[0176] Embodiment 2, the pharmacodynamic research of velvet antler protein extract

[0177] 1 material

[0178] 1.1 Experimental animals

[0179] Male C57BL / 6 mice [provided by Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., the animal license number is SCXK (Beijing) 2012-0001], 8 weeks, were raised in an SPF animal room.

[0180] 1.2 Instrument reagents

[0181] Losartan Potassium (Kosoya K021048); Automatic Biochemical Analyzer (Hitachi 7080); CK Kit (150601 of Zhongsheng Beikong Biotechnology Co., Ltd.); CKMB Kit (150761 of Zhongsheng Beikong Biotechnology Co., Ltd.); LDHKit ( Zhongsheng Beikong Biotechnology Co., Ltd. 140571); Masson staining kit (Suolaibao DC0032-100).

[0182] 2 methods

[0183] 2.1 Preparation of velvet antler protein extract

[0184] The velvet protein extract is prepared according to the following method, and the velvet protein extract is the velvet protein extract for preventing and / or treating Parkinson's disease. details as f...

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Abstract

The invention provides velvet antler protein extract. The velvet antler protein extract is characterized by being obtained through the following method which comprises the steps that 1, velvet antler is smashed to obtain a velvet antler smashed material, water extraction is conducted on the velvet antler smashed material, water-soluble matter is collected, and velvet antler water-soluble extract is obtained; 2, the velvet antler water-soluble extract is dialyzed through water till the pH value ranges from 6.5 to 8.5, dialysis is completed, and the velvet antler protein extract is obtained. The velvet antler protein extract can be used for preventing and curing myocardial infarction.

Description

[0001] This application claims the priority of the Chinese invention patent application No. 201510408556.9 entitled "Preparation method of deer antler extract and related products" filed by the same applicant on July 13, 2015. technical field [0002] The application relates to an extract of Chinese medicinal materials and its pharmaceutical use, specifically an antler protein extract and its pharmaceutical use. Background technique [0003] Deer antler is the unossified and densely hairy young horn of the sika deer or red deer, an animal of the chordate phylum Mammalia deer family. Antler was first recorded in "Shen Nong's Materia Medica", listed as a Chinese medicine, and is one of the traditional Chinese medicines in my country. Warm in nature, sweet in taste, salty in liver and kidney channels. Functions and indications: strengthen Yuanyang, invigorate qi and blood, benefit essence, strengthen muscles and bones. [0004] Antler is the only appendage in mammals with ful...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K2/00C07K1/14C07K1/34C12N9/00A61K38/02A61K38/43A61P9/10
CPCA61K38/00C07K1/14C07K1/34C07K2/00C12N9/00
Inventor 王毅孙娅楠李超华马淑骅
Owner EXPERIMENTAL RES CENT CHINA ACAD OF CHINESE MEDICAL SCI
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