Antler protein extract and its medicinal application
A protein extract and extract technology, applied in the direction of peptide/protein components, biochemical equipment and methods, drug combination, etc., can solve the problem of less protective effect on myocardial injury
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Embodiment 1
[0095] Embodiment 1, preparation and detection of velvet antler protein extract
[0096] 1. Preparation of deer antler protein extract
[0097] Prepare deer antler protein extract according to the following method:
[0098] The fresh velvet antler is directly crushed for the first time with a pulverizer to obtain the first crushed velvet antler. Put the velvet antler crushed for the first time into liquid nitrogen quick-freezing, put it into a superfine pulverizer, add an appropriate amount of water for the second crushing until it is minced meat, and obtain the second crushed velvet antler, the molecular particle size of the second crushed velvet antler is 75 μm. Add a certain amount of water to the second crushed velvet antler (the mass ratio of the second crushed velvet to water is 1:5), then add an appropriate amount of glacial acetic acid to keep the pH at 3.5, keep it at 4°C for 24 hours, and then centrifuge at 4000g for 10 minutes to separate The supernatant is clear...
Embodiment 2
[0176] Embodiment 2, the pharmacodynamic research of velvet antler protein extract
[0177] 1 material
[0178] 1.1 Experimental animals
[0179] Male C57BL / 6 mice [provided by Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., the animal license number is SCXK (Beijing) 2012-0001], 8 weeks, were raised in an SPF animal room.
[0180] 1.2 Instrument reagents
[0181] Losartan Potassium (Kosoya K021048); Automatic Biochemical Analyzer (Hitachi 7080); CK Kit (150601 of Zhongsheng Beikong Biotechnology Co., Ltd.); CKMB Kit (150761 of Zhongsheng Beikong Biotechnology Co., Ltd.); LDHKit ( Zhongsheng Beikong Biotechnology Co., Ltd. 140571); Masson staining kit (Suolaibao DC0032-100).
[0182] 2 methods
[0183] 2.1 Preparation of velvet antler protein extract
[0184] The velvet protein extract is prepared according to the following method, and the velvet protein extract is the velvet protein extract for preventing and / or treating Parkinson's disease. details as f...
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