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A kind of electrophoresis buffer for cell in situ electrophoresis

A technology of electrophoresis buffer and in-situ electrophoresis, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of not maintaining stress balance electrophoresis buffer, and achieve the effect of easy recycling

Inactive Publication Date: 2017-08-08
SHANDONG TUMOR HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Maintaining cell stress balance during electrophoresis is a prerequisite for cell in situ electrophoresis-related detection, but there is currently no electrophoresis buffer that maintains cell stress balance

Method used

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  • A kind of electrophoresis buffer for cell in situ electrophoresis
  • A kind of electrophoresis buffer for cell in situ electrophoresis

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Embodiment 1

[0016] An electrophoresis buffer for cell in situ electrophoresis, pH is 8.3, solvent is water, solute is composed of Tris, Glycine, Glucose, wherein the concentration Tris: 25mM; Glycine: 192mM; Glucose: 43.2mM.

[0017] Buffer application:

[0018] The device used: including the cell electrophoresis tank 4: the tank is a polystyrene circular tube with an inner diameter of 0.8 cm, an outer diameter of 0.95 cm and a length of 18 cm. In the middle of the tube wall, there is a 9×0.6cm rectangular notch along its longitudinal axis; electrode buffer tank 1: one for the anode and one for the cathode; the same size, 10×3.5×5cm; material: polystyrene; side connection Channel 3: one on the anode side and one on the cathode side; the same size, 0.8cm inner diameter, 0.95cm outer diameter, and 10cm long silicone tube; filter membrane 2: one on the anode side and one cathode side; the same size, 0.2μm or 0.45μm Hydrophilic polyethersulfone filter membrane; electrodes: one anode and one ...

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Abstract

The invention discloses an electrophoresis buffer for cell in situ electrophoresis, the pH is 8.3, the solvent is water, and the solute is composed of Tris, Glycine and Glucose, wherein the concentration of Tris: 25mM; Glycine: 192mM; Glucose: 43.2mM. Mix the corresponding weight of Tris, Glycine, Glucose with water. The electrophoresis buffer of the present invention can maintain the stress balance of the cells under the condition of maintaining the isotonicity of the cells, select non-polar glucose, and be easy to separate in subsequent centrifugation, cell recovery and the like.

Description

technical field [0001] The invention relates to an electrophoresis buffer for cell in situ electrophoresis. Background technique [0002] Cell electrophoresis (cell electrophoresis), the cells are prepared into a suspension solution, so that a single free cell is dispersed in an isotonic medium. Under the action of an electric field, the cells move in the electrophoresis chamber. This phenomenon is called cell electrophoresis. Cell in situ electrophoresis refers to an electrophoresis method that separates the substances in the cell from the cell by electrophoresis after the cell membrane and nucleus are treated under the condition of maintaining cell stress balance. Maintaining cell stress balance during electrophoresis is a prerequisite for cell in situ electrophoresis-related detection, but currently there is no electrophoresis buffer that maintains cell stress balance. Contents of the invention [0003] The object of the present invention is to solve the above problems...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/561
Inventor 国前于金明廖湘鲁王兴武宋现让
Owner SHANDONG TUMOR HOSPITAL
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