Primer set, kit and detection method for multiplex PCR detection of three types of pathogenic bacteria in food

Abstract

The present invention belongs to the field of food microorganism safety detection, and provides a multiplex PCR detection primer set, a multiplex PCR detection kit and a multiplex PCR detection method for Staphylococcus aureus, Salmonella and Shigella in food. According to the present invention, with the multiplex PCR detection primer set, the multiplex PCR detection kit and the multiplex PCR detection method, the species-specific identification genes and the drug resistance associated genes of the Staphylococcus aureus, the Salmonella and the Shigella are combined and utilized, such that the technical effects of wide detection covering range, strong specificity and high sensitivity can be achieved, the advantages of short detection time consumption, simple operation, low equipment requirement, high throughput, and saving of a lot of manpower, materials and financial resources are provided, and the primer set, the kit and the detection method meet the requirements of the rapid detection, and are easily promoted and applied in the actual production.

Description

technical field [0001] The invention belongs to the field of food microbe safety detection, and relates to a primer set, a kit and a method for rapidly detecting and identifying pathogenic bacteria in food by using multiple PCR technology. Specifically, the present invention relates to a multiplex PCR detection primer set, kit and detection method for Staphylococcus aureus, Salmonella sp. and Shigella sp. in foods. Background technique [0002] In recent years, food safety incidents have occurred frequently, and food safety issues have become more and more concerned and valued by the public. Many food-borne pathogens are transmitted through water and food and cause food-borne diseases, and their morbidity and mortality are gradually increasing. Worldwide, more than 30% of the population is infected with foodborne illness each year, costing billions of dollars. Preventing and controlling the occurrence and spread of foodborne diseases has become one of the important measure...

AI Technical Summary

Problems solved by technology

It can be seen that the existing technology cannot fully meet the needs of national food safety standards

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