Primer set, kit and detection method for multiplex PCR detection of Staphylococcus aureus in food

Abstract

The invention is used for the field of food microorganism safe detection, and provides a primer group used for multiple PCR detection by aiming at staphylococcus aureus in food, a kit and a detection method thereof. The primer group used for the multiple PCR detection, the kit and the detection method can combine and use species-specificity discrimination gene and drug resistance-related gene, and the technical effects of wide detection cover scope, strong specificity and high sensitivity can be realized, detection time consumption is short, operation is simple, equipment requirement is low, manpower, material resource and financial resource can be saved; the primer group used for the multiple PCR detection, the kit and the detection method are suitable for rapid detection requirement, and are easily popularized and applied in practical production.

Description

technical field [0001] The invention belongs to the field of food microbe safety detection, and relates to a primer set, a kit and a method for rapidly detecting and identifying pathogenic bacteria in food by using multiple PCR technology. Specifically, the present invention relates to a primer set, a kit and a detection method for multiplex PCR detection of Staphylococcus aureus in food. Background technique [0002] In recent years, food safety incidents have occurred frequently, and food safety issues have become more and more concerned and valued by the public. Many food-borne pathogens are transmitted through water and food and cause food-borne diseases, and their morbidity and mortality are gradually increasing. Worldwide, more than 30% of the population is infected with foodborne illness each year, costing billions of dollars. Preventing and controlling the occurrence and spread of foodborne diseases has become one of the important measures to solve social food safe...

AI Technical Summary

Problems solved by technology

However, when the selected target genes are too specific (the distribution range is too narrow), such as drug resistance-related genes and toxin genes, which are mainly used for medical detection purposes, as most existing methods focus on, it will cause detection Insufficient coverage types, resulting in missed detection and other problems; on the contrary, it may cause disadvantages such as high false positive rate

Therefore, the selection of target genes still needs to be optimized. Specifically, how to select target genes with high coverage and high specificity for all strains of Staphylococcus aureus is still a difficult problem in multiplex PCR detection

[0007] In addition, considering that only a small amount of Staphylococcus aureus in food can cause foodborne diseases, there are also high requirements for the sensitivity of the detection method

The detection sensitivity of the existing PCR technology for Staphylococcus aureus is usually around 10-100pg / μL, which cannot fully meet the requirements of the national food safety standards

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