Enzyme linked immunosorbent assay kit for detecting methimazole residues in animal-derived food and application thereof

An enzyme-linked immunosorbent reagent and methimazole technology, which is applied to measurement devices, instruments, scientific instruments and other directions, can solve problems such as being unsuitable for on-site monitoring and screening, cumbersome and high-skilled requirements, and complexity, and achieve convenient and easy-to-implement testing methods. The detection method is efficient and accurate, and the effect of high precision

Inactive Publication Date: 2016-04-13
JIANGSU WISE SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the method for detecting methimazole residues is mainly high-performance liquid chromatography (HPLC), which is not suitable for on-s

Method used

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  • Enzyme linked immunosorbent assay kit for detecting methimazole residues in animal-derived food and application thereof
  • Enzyme linked immunosorbent assay kit for detecting methimazole residues in animal-derived food and application thereof
  • Enzyme linked immunosorbent assay kit for detecting methimazole residues in animal-derived food and application thereof

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Experimental program
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Embodiment Construction

[0044] The present invention will be further elaborated below in conjunction with specific implementation steps.

[0045] 1. Preparation of ELISA plate

[0046] Dilute the methimazole antigen to 0.20 μg / mL with coating buffer, add 100 μl to each well, incubate at 37°C for two hours, pour off the coating solution, wash twice with diluted concentrated washing solution, each time for 30 seconds, tap After drying, add 200 μl of blocking solution to each well, incubate at 37°C for 2 hours, pour off the liquid in the well, and store in a vacuum-sealed vacuum with an aluminum film after drying.

[0047] 2. Set up an enzyme-linked immunosorbent assay kit for detecting methimazole residues to include the following components:

[0048] (1) ELISA plate coated with methimazole antigen

[0049] (2) Goat anti-mouse anti-antibody labeled with horseradish peroxidase

[0050] (3) Methimazole antibody working solution

[0051] (4) 6 bottles of methimazole standard substance, the concentrati...

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Abstract

The invention relates to an enzyme linked immunosorbent assay kit for detecting methimazole residues in animal-derived food. The kit comprises the following components according to proportion: a coating plate, a standard substance, an enzyme-labeled substrate working solution, a substrate solution A, a substrate solution B, a terminating solution, a concentrated washing liquid and a concentrated extract. The kit sensitivity is 0.3 ppb, and the precision is as follows: intra assay variation coefficient (CV%) is 5%, and inter assay variation coefficient (CV%) is 10%; the accuracy is shown by recovery rate, and the recovery rate is in the range of 80-110%. IC 50 range is between 0.3 and 0.5 ppb, and the linear correlation coefficient |r| is not less than 0.9900, and the kit has the advantages of wide usage scope, convenient usage and accurate detection.

Description

technical field [0001] The invention relates to an enzyme-linked immunosorbent detection technology, in particular to an enzyme-linked immunosorbent assay kit for detecting methimazole, which is particularly suitable for detecting methimazole residues in animal source foods. technical background [0002] Methimazole is a commonly used drug for the treatment of hyperthyroidism, which can change the endocrine in animals. After feeding animals with feed containing methimazole, there will be residues in urine, chicken and tissues to varying degrees. Countries such as the European Union have clearly It is listed as a prohibited drug in zoo food. At present, hormones have been banned from being used in aquaculture by most countries, and cannot be detected in food. However, the domestic detection of methimazole and other prohibited drug residues in animal products is almost blank. According to my country's regulations on the monitoring of animal source product residues and the req...

Claims

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Application Information

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IPC IPC(8): G01N33/577
Inventor 杜道林洪霞刘静
Owner JIANGSU WISE SCI & TECH DEV
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