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Novel plant tissue culture flask

A new type of plant tissue culture technology, applied in plant regeneration, botanical equipment and methods, horticulture, etc., can solve problems such as easily damaged plants, unchanged bottle operation, unclear experimental records, etc. Photosynthesis, the effect of providing equipment protection

Inactive Publication Date: 2016-04-20
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention intends to provide a new type of culture bottle to solve the defects of unchanging operation, unclear experimental records and easy damage to plants when transplanting due to the integrally formed bottle body

Method used

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  • Novel plant tissue culture flask
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Embodiment 1

[0021] Embodiment 1: as figure 1 Shown: a new type of plant tissue culture bottle, including a bottle cover 1 and a bottle cylinder 3 that are mutually socketed and detachable. The bottle cover 1 includes a cylindrical bottle body 105 and an arc top 102 integrally formed with the bottle body 105. The arc top The middle part of 102 is connected with air inlet pipe 103, and the bottom of bottle body 105 and the junction with arc top 102 is provided with air outlet pipe 106; Cylinder 3 is relatively sealed, and the bottom of described bottle cylinder 3 is connected with bottle holder 4, and the bottom of bottle body 105 is provided with internal thread section, and the top of internal thread section is provided with sealing ring; A microporous bacteria filter 2 is provided, and the diameter of micropores in the microporous bacteria filter 2 is 0.22um; the mouth of the air inlet pipe 103 and the outlet pipe 106 is also connected with a conical closed cover 104; the bottle cover 1 ...

Embodiment 2

[0024] The difference between embodiment 2 and embodiment 1 is that the height of the bottle cylinder 3 is 1 / 2 times of the height of the bottle body 105, and the material of the bottle cover 1 is opaque high-temperature-resistant plastic; High temperature glass.

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Abstract

The invention discloses a novel plant tissue culture flask. The novel plant tissue culture flask comprises a flask hood and a flask cylinder. The flask hood is detachably connected to the upper portion of the flask cylinder in a sealing mode. The flask hood comprises a cylindrical flask body and an arc top integrally formed with the flask body. An air inlet pipe is arranged in the middle of the arc top, and an air outlet pipe is arranged on the flask body. The height of the flask cylinder is 1 / 3-1 / 2 that of the flask body, and a flask holder is connected to the bottom of the flask cylinder. An internal thread segment is arranged on the flask body, and the flask cylinder is provided with an external thread segment meshed with the internal thread segment. The air inlet pipe and the air outlet pipe are both provided with a detachable micropore bacterial filter. Pipe ports of the air inlet pipe and the air outlet pipe are provided with circular-truncated-cone-shaped closing covers connected through a flexible piece. The combined split closed culture flask is designed, the flask hood and the flask cylinder are of a split structure, the size of the flask cylinder is equal to a pipe diameter, when operation, experimental recording and photographing and acclimatization and transplanting are needed, the flask hood and the flask cylinder are separated, and the novel plant tissue culture flask has the advantages that operation can be conveniently performed under direct vision, and roots, stems and leaves of plants cannot be easily damaged.

Description

technical field [0001] The invention relates to the technical field of tissue culture plant regeneration, in particular to a novel plant tissue culture bottle. Background technique [0002] In 1902, German botanist Haberlandt proposed the theory of "plant cell totipotency". In 1958, American botanist Stewart and others used carrot phloem cells to cultivate and obtained a complete plant capable of flowering and fruiting, confirming the "totipotency of plant cells". The prediction of "cell totipotency" has created a new technology - plant tissue culture technology, which is also called "three-dimensional culture" in a broad sense. Plant tissue culture technology is not only an important means for asexual reproduction and rapid seedling propagation, but also an important part of technical systems such as cell hybridization and genetic improvement. By establishing a suitable rapid tissue culture system, it not only solves the practical problem of the depletion of its wild resou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 徐德林沈访钱刚
Owner ZUNYI MEDICAL UNIVERSITY
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