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Tracheids are elongated cells in the xylem of vascular plants that serve in the transport of water and mineral salts. Tracheids are one of two types of tracheary elements, vessel elements being the other. Tracheids, unlike vessel elements, do not have perforation plates.
A method for accurately measuring the wall thickness of a scots pine tracheid based on a micro-CT image, a scots pine high-carbon fixation strain breeding method and system
This invention discloses a method for accurately measuring tracheid wall thickness in *Pinus sylvestris* based on micro-CT images, a method for selecting high carbon-fixing varieties of *Pinus sylvestris*, and a system, belonging to the field of forest tree genetics and breeding technology. Addressing the systematic underestimation of tracheid wall thickness due to partial volume effect in micro-CT measurements, this invention employs the gray-scale centroid method to calculate the sub-pixel position of the wall-cavity boundary, reducing the measurement error from ±1.5 μm to ±0.3 μm. Based on accurate wall thickness data, screening criteria are established: "average wall thickness ≥ 75th percentile of the population, coefficient of variation ≤ 15%, and proportion of thick-walled tracheids ≥ 60%". Combined with carbon storage index verification, this enables precise selection of high carbon-fixing varieties. This method is non-destructive, efficient, and reproducible, and is applicable to the genetic improvement of *Pinus sylvestris* and similar coniferous trees.
Owner:SHENYANG AGRI UNIV