Culture medium and induction method for improving saffron callus induction rate

A callus induction and culture medium technology, applied in the field of plant tissue culture, can solve the problems affecting the fast propagation efficiency of saffron tissue culture, waste of explants, low induction rate of saffron, etc., so as to promote the induction of saffron callus, promote the The effect of dedifferentiation

Inactive Publication Date: 2016-04-27
FOSHAN JINLANLING EDUCATION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the current rapid propagation methods of saffron adopt tissue culture, but the induction rate of saffron in the first explant induction of the current tissue culture is very low, resulting in a large amount of waste of explants and seriously affecting the tissue culture and rapid propagation efficiency of saffron

Method used

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  • Culture medium and induction method for improving saffron callus induction rate
  • Culture medium and induction method for improving saffron callus induction rate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: Preparation of a culture medium for improving the induction rate of saffron callus.

[0017] According to the MS medium composition and consumption shown in Table 1 and the medium composition and consumption of improving the Saffron callus induction rate shown in Table 2, prepare the medium for improving the saffron callus induction rate of the present invention.

[0018] Use a balance to weigh the components in the MS medium, PAA (phenylacetic acid), 6-BA (6-benzyl adenine), banana puree, sucrose and agar powder, put them in a triangular flask, add an appropriate amount of distilled water, and stir to dissolve , and adjust the pH to 6.0 with HCL and KOH, and set the volume to 1000 mL. Seal the flask, place it in an autoclave, and sterilize it at 121°C for 20 minutes, then place it in an ultra-clean workbench to cool naturally, and set it aside.

[0019] When cooled to 50-60°C, divide the culture medium into tissue culture bottles, let it cool in the air,...

Embodiment 2

[0024] Embodiment two: Saffron corm induction culture.

[0025] Prepare the medium for improving the saffron callus induction rate by the method described in Example 1, and set aside.

[0026] Take fresh and healthy saffron bulbs that were born in the current year as explants, place them in a refrigerator at -4°C for 48 hours of vernalization at low temperature, then take out the bulbs and rinse them with water, dry the surface water with filter paper, and set aside.

[0027] Shake and soak the bulblet obtained above with 75% alcohol for 30 seconds, take out the bulblet, rinse it with distilled water for 3 times, and then use 1% HgCl 2 Soak for 4 minutes, then take out the small bulbs, rinse them with distilled water for 3 times, then soak them in 10mg / LPAA (phenylacetic acid) solution for 5 minutes, take out the small bulbs, and set aside.

[0028] The bulblet of above-mentioned gained is cut into the fritter of 0.5-1 centimeter with sterile scalpel, and inoculate in the cul...

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Abstract

The invention provides a culture medium and an induction method for improving saffron callus induction rate, and relates to the technical field of plant tissue culture. According to the culture medium, an MS culture medium is adopted as a base culture medium, and PAA (phenylacetic acid), 6-BA (6-benzyladenine), mashed banana, sucrose and agar powder are added. According to the method, during explant disinfection, PAA (phenylacetic acid) is used as a disinfectant. The culture medium and induction method provided by the invention have the advantages of high explant survival rate and high induction rate.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and particularly relates to a culture medium for increasing the induction rate of saffron callus, and also relates to an induction method for increasing the induction rate of saffron callus. Background technique [0002] Saffron is a kind of precious Chinese herbal medicine. Because it is only used as medicine on the stigma, the yield is extremely low. At the same time, because of its extremely limited resources, but its wide range of uses, it is expensive. It has always been known as "plant gold", so the rapid propagation of saffron Research is extremely important. [0003] Most of the current rapid propagation methods of saffron adopt tissue culture, but the induction rate of saffron in the first explant induction of the current tissue culture is very low, resulting in a large amount of waste of explants and seriously affecting the tissue culture and rapid propagation efficiency of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 何志铿
Owner FOSHAN JINLANLING EDUCATION TECH
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